ID   NF314_NAEFO             Reviewed;         482 AA.
AC   P42661;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Virulence-related protein Nf314;
DE            EC=3.4.16.-;
OS   Naegleria fowleri (Brain eating amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5763;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=ATCC 30894 / Lee;
RX   PubMed=1587609; DOI=10.1128/iai.60.6.2418-2424.1992;
RA   Hu W.-N., Kopachik W., Band R.N.;
RT   "Cloning and characterization of transcripts showing virulence-related gene
RT   expression in Naegleria fowleri.";
RL   Infect. Immun. 60:2418-2424(1992).
CC   -!- FUNCTION: May be required but is not sufficient for increased
CC       virulence.
CC   -!- INDUCTION: By growth on mammalian cells.
CC   -!- SIMILARITY: Belongs to the peptidase S10 family. {ECO:0000305}.
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DR   EMBL; M88397; AAA29384.1; -; mRNA.
DR   PIR; A43828; A43828.
DR   AlphaFoldDB; P42661; -.
DR   SMR; P42661; -.
DR   ESTHER; naefo-nf314; Carboxypeptidase_S10.
DR   VEuPathDB; AmoebaDB:FDP41_000254; -.
DR   VEuPathDB; AmoebaDB:NF0060860; -.
DR   VEuPathDB; AmoebaDB:NfTy_024570; -.
DR   GO; GO:0004185; F:serine-type carboxypeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR001563; Peptidase_S10.
DR   InterPro; IPR033124; Ser_caboxypep_his_AS.
DR   InterPro; IPR018202; Ser_caboxypep_ser_AS.
DR   PANTHER; PTHR11802; PTHR11802; 2.
DR   Pfam; PF00450; Peptidase_S10; 1.
DR   PRINTS; PR00724; CRBOXYPTASEC.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00560; CARBOXYPEPT_SER_HIS; 1.
DR   PROSITE; PS00131; CARBOXYPEPT_SER_SER; 1.
PE   2: Evidence at transcript level;
KW   Carboxypeptidase; Hydrolase; Protease.
FT   CHAIN           1..482
FT                   /note="Virulence-related protein Nf314"
FT                   /id="PRO_0000120567"
FT   ACT_SITE        163
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        399
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        459
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   482 AA;  53848 MW;  0B83049C1A8A0908 CRC64;
     MMMRCVNGQT AQDHLVTQLP GLSGNIGVKS YTGYLLANAT RGRYLFYWFF ESMRNPSQDP
     LVMWTNGGPG CSSLGGEASE HGLFLVNADG ATITRNPYSW NRVSNILYIE QPVGVGFSYS
     NSTDDYQNLN DVQAASDMNN ALRDFLTRFP QFIGRETYLA GESYGGVYVP TTAYNIVEGN
     GKGQQPYVNL VGILVGNGVT DAEADSNSIP PMMKYHSLIS IKYYEEGYKA CQGDFYANQN
     LPACQKFLTD SSNAMGNINP YYIYDSCPWL GINLQQKLKT TQEMTFQVLD PKTQQPVKIH
     PLFQMYKHGG WSKRVANERN FAPRFETDAP CVPNQSIAKY FRRLDVQQAL GVRRKTADPN
     GWNICTGIIN YTQVYSTILP FYAKLLPHIR ILVYSGDTDM VVNGLGTQAA IDKLQLQETS
     SWRTWEFDSA LGTVVGGYIR KFEKSGKGLT FITVRGAGHM VPLVKPDSAF YMFKNFIDGT
     CC