ID   NFAA_DICDI              Reviewed;         920 AA.
AC   Q55AR6;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Neurofibromin-A;
DE            Short=DdNF1;
GN   Name=nfaA; ORFNames=DDB_G0271750;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=18948008; DOI=10.1016/j.cub.2008.08.069;
RA   Zhang S., Charest P.G., Firtel R.A.;
RT   "Spatiotemporal regulation of Ras activity provides directional sensing.";
RL   Curr. Biol. 18:1587-1593(2008).
CC   -!- FUNCTION: Regulator of the GTPase activity of Ras, mainly RasG and
CC       RasB. {ECO:0000269|PubMed:18948008}.
CC   -!- DISRUPTION PHENOTYPE: Show spatially and temporally unregulated Ras
CC       activity causing cytokinesis and chemotaxis defects.
CC       {ECO:0000269|PubMed:18948008}.
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DR   EMBL; AAFI02000006; EAL71565.1; -; Genomic_DNA.
DR   RefSeq; XP_645456.1; XM_640364.1.
DR   AlphaFoldDB; Q55AR6; -.
DR   SMR; Q55AR6; -.
DR   STRING; 44689.DDB0233763; -.
DR   PaxDb; Q55AR6; -.
DR   PRIDE; Q55AR6; -.
DR   EnsemblProtists; EAL71565; EAL71565; DDB_G0271750.
DR   GeneID; 8618084; -.
DR   KEGG; ddi:DDB_G0271750; -.
DR   dictyBase; DDB_G0271750; nfaA.
DR   eggNOG; KOG1826; Eukaryota.
DR   HOGENOM; CLU_316988_0_0_1; -.
DR   InParanoid; Q55AR6; -.
DR   OMA; IRTICNC; -.
DR   Reactome; R-DDI-5658442; Regulation of RAS by GAPs.
DR   PRO; PR:Q55AR6; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0009898; C:cytoplasmic side of plasma membrane; IDA:dictyBase.
DR   GO; GO:0005096; F:GTPase activator activity; IMP:dictyBase.
DR   GO; GO:0090630; P:activation of GTPase activity; IMP:dictyBase.
DR   GO; GO:0030833; P:regulation of actin filament polymerization; IMP:dictyBase.
DR   GO; GO:0050920; P:regulation of chemotaxis; IMP:dictyBase.
DR   GO; GO:0051896; P:regulation of protein kinase B signaling; IMP:dictyBase.
DR   GO; GO:0046578; P:regulation of Ras protein signal transduction; IMP:dictyBase.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   CDD; cd00170; SEC14; 1.
DR   Gene3D; 1.10.506.10; -; 1.
DR   Gene3D; 3.40.525.10; -; 1.
DR   InterPro; IPR001251; CRAL-TRIO_dom.
DR   InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR   InterPro; IPR039360; Ras_GTPase.
DR   InterPro; IPR001936; RasGAP_dom.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   PANTHER; PTHR10194; PTHR10194; 1.
DR   Pfam; PF13716; CRAL_TRIO_2; 1.
DR   Pfam; PF00616; RasGAP; 1.
DR   SMART; SM00323; RasGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   SUPFAM; SSF52087; SSF52087; 1.
DR   PROSITE; PS50191; CRAL_TRIO; 1.
DR   PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
PE   4: Predicted;
KW   GTPase activation; Reference proteome.
FT   CHAIN           1..920
FT                   /note="Neurofibromin-A"
FT                   /id="PRO_0000389431"
FT   DOMAIN          47..259
FT                   /note="Ras-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
FT   DOMAIN          512..673
FT                   /note="CRAL-TRIO"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00056"
FT   REGION          344..405
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          477..508
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        346..405
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        479..496
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   920 AA;  103972 MW;  4F7237CBB456A290 CRC64;
     MDVDKQMLQW RKRAWLNSNQ LYGELTDIII KDTGFTSSFA NLIDNSEVDS FSKNIVNIFT
     TANKTLPLIK DLIYNEFNTK VLAEGEGSIL RGNSIVNKIE GAYVRLIGAN YLRFVLSDLV
     TKVVLDSDLK LEIDPRKLND YYEDKIEFEK KVAEVELRDN QKSLHNIAQQ FLDRITDPSV
     VVEMPREIRA IADYTAESAL RYAPESLAPL VGGFIMLRFF SPAIVTPEYS KLLSSEVAPS
     KRAKRNLVLL AKVLQNASNG VLFGGKEEFM TCMNDFIIGN KEKMSAYFNL ICKDPLNQNN
     KWSDLEGVKS ATTTTTILTP TNTTTSSNGS GGTTWAKPIV GGSKWLATTP SGNTPSPAIS
     NASSAHNGKS NNTTNNNNNN NNNNNNNNNN NNNNNNNSNK TTTTATTISS NSWDVYIKNV
     QIQDLFDLHR IFDSYKEKIV NKIINTNNNN ANNANNNNTN NINNKTAIRV IEILKELGPS
     PKTKTERKKK ETDAAEEPTT SLQNGASMGS AFDECTHMLE RSRFLFQGPN DKNDRSVFYL
     IVNRVKPEVF DNVNPLIAHI FKVMDICVNS PYTLVVDMSW AHISNDLKKA IFTHLPKLAE
     IFSRKYKKNI DKIFIVHPSA YTRAVIYFMS AFTSRKLKRK IHDIYNWKDL SQYIDIENIA
     LPETSKDFIT KSYRVVKVNS KGKRQERLIK FTSNSLLNID PKTHRIQNEK RINEIDEITS
     RLGSIEINMK LSGESKKSGS ILGTKIGFLS INNNNNNNND DSNGNHDINE SNSRKYICNH
     ELERDHILQD IFETGFKMGL QSKSTKSLPT EYKVIKVNNV GKHQERIFKL TIDSLLNLDQ
     QRIKSENSFA GIEEVILDPH DDDIVWLKFK SEPSRRKIIC TVKGEGKLLL EVLTDAINKY
     QTTIDIQEGA LKLDAEEGFL