ID   NFCP_CONGI              Reviewed;         227 AA.
AC   Q95W86; Q95W11;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=GFP-like non-fluorescent chromoprotein {ECO:0000303|PubMed:11682051};
DE   AltName: Full=cgCP {ECO:0000303|PubMed:11682051};
DE   AltName: Full=cpCP {ECO:0000303|PubMed:11682051};
OS   Condylactis gigantea (Giant Caribbean anemone) (Condylactis passiflora).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Condylactis.
OX   NCBI_TaxID=47073;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAL27537.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND MUTAGENESIS OF CYS-143.
RX   PubMed=11682051; DOI=10.1016/s0014-5793(01)02930-1;
RA   Gurskaya N.G., Fradkov A.F., Terskikh A., Matz M.V., Labas Y.A.,
RA   Martynov V.I., Yanushevich Y.G., Lukyanov K.A., Lukyanov S.A.;
RT   "GFP-like chromoproteins as a source of far-red fluorescent proteins.";
RL   FEBS Lett. 507:16-20(2001).
CC   -!- FUNCTION: Non-fluorescent pigment protein that is mauve in color. The
CC       wild-type form is non-fluorescent.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Absorption:
CC         Abs(max)=571 nm;
CC   -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11682051}.
CC   -!- PTM: Contains a chromophore consisting of modified amino acid residues.
CC       The chromophore is formed by autocatalytic backbone condensation
CC       between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to
CC       didehydrotyrosine. Maturation of the chromophore requires nothing other
CC       than molecular oxygen. The precise stereochemistry of the tyrosine has
CC       not been determined.
CC   -!- BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitous
CC       tool for making chimeric proteins, where they function as a fluorescent
CC       protein tag. Typically they tolerate N- and C-terminal fusion to a
CC       broad variety of proteins. They have been expressed in most known cell
CC       types and are used as a noninvasive fluorescent marker in living cells
CC       and organisms. They enable a wide range of applications where they have
CC       functioned as a cell lineage tracer, reporter of gene expression, or as
CC       a measure of protein-protein interactions. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Fluorescence excitation of the Ser-143 mutant is at 587
CC       nm and emission at 622 nm.
CC   -!- SIMILARITY: Belongs to the GFP family. {ECO:0000305}.
CC   -!- CAUTION: Gurskaya et al., 2001 describe 2 different proteins coming
CC       from C.gigantea (cgCP) and C.passiflora (cpCP). Since both C.passiflora
CC       and C.passiflora species are synonym, the 2 proteins are merged and
CC       some conflicts are reported. {ECO:0000305|PubMed:11682051}.
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DR   EMBL; AF363775; AAL27537.1; -; mRNA.
DR   EMBL; AF383155; AAL27541.1; -; mRNA.
DR   AlphaFoldDB; Q95W86; -.
DR   SMR; Q95W86; -.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.155.10; -; 1.
DR   InterPro; IPR009017; GFP.
DR   InterPro; IPR011584; GFP-related.
DR   Pfam; PF01353; GFP; 1.
DR   SUPFAM; SSF54511; SSF54511; 1.
PE   1: Evidence at protein level;
KW   Chromophore; Luminescence; Photoprotein.
FT   CHAIN           1..227
FT                   /note="GFP-like non-fluorescent chromoprotein"
FT                   /id="PRO_0000192583"
FT   MOD_RES         64
FT                   /note="2,3-didehydrotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P83690"
FT   CROSSLNK        63..65
FT                   /note="5-imidazolinone (Ala-Gly)"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         143
FT                   /note="C->S: Produces a fluorescent form."
FT                   /evidence="ECO:0000269|PubMed:11682051"
FT   CONFLICT        35
FT                   /note="F -> Y (in Ref. 1; AAL27541)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        116
FT                   /note="I -> N (in Ref. 1; AAL27541)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        135
FT                   /note="K -> N (in Ref. 1; AAL27541)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        222
FT                   /note="V -> L (in Ref. 1; AAL27541)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        227
FT                   /note="T -> N (in Ref. 1; AAL27541)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   227 AA;  25416 MW;  BCFA5A4CBCE1B3F7 CRC64;
     MAGLLKESMR IKIYMEGTVN GYHFKCEGEG DGNPFEGTQN MRIRVTEGAP LPFAFDILSP
     CCAYGSKTFI KHTSGIPDYF KQSFPEGFTW ERTTIYEDGG VLTAHQDTSL EGNCLIYKVK
     VLGTNFPADG PVMKKISGGW EPCTEIVYQD NGVLRGRNVM ALKVSGRPPL ICHLHSTYRS
     KKACALTMPG FHFADLRIQM PKKKKDEYFE LYEASVARYS DVPEKAT