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NFCP_GONTE
ID   NFCP_GONTE              Reviewed;         221 AA.
AC   Q95P04;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=GFP-like non-fluorescent chromoprotein;
DE            Short=gtCP;
OS   Goniopora tenuidens (Anemone coral) (Rhodaraea tenuidens).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Scleractinia;
OC   Fungiina; Poritidae; Goniopora.
OX   NCBI_TaxID=75301;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAL27542.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND MUTAGENESIS OF ASN-142.
RX   PubMed=11682051; DOI=10.1016/s0014-5793(01)02930-1;
RA   Gurskaya N.G., Fradkov A.F., Terskikh A., Matz M.V., Labas Y.A.,
RA   Martynov V.I., Yanushevich Y.G., Lukyanov K.A., Lukyanov S.A.;
RT   "GFP-like chromoproteins as a source of far-red fluorescent proteins.";
RL   FEBS Lett. 507:16-20(2001).
CC   -!- FUNCTION: Non-fluorescent pigment protein that is lilac in color.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Absorption:
CC         Abs(max)=580 nm;
CC   -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11682051}.
CC   -!- PTM: Contains a chromophore consisting of modified amino acid residues.
CC       The chromophore is formed by autocatalytic backbone condensation
CC       between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to
CC       didehydrotyrosine, and formation of a double bond to the alpha-amino
CC       nitrogen of residue Xaa-N. Maturation of the chromophore requires
CC       nothing other than molecular oxygen. The precise stereochemistry of the
CC       tyrosine has not been determined.
CC   -!- BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitous
CC       tool for making chimeric proteins, where they function as a fluorescent
CC       protein tag. Typically they tolerate N- and C-terminal fusion to a
CC       broad variety of proteins. They have been expressed in most known cell
CC       types and are used as a noninvasive fluorescent marker in living cells
CC       and organisms. They enable a wide range of applications where they have
CC       functioned as a cell lineage tracer, reporter of gene expression, or as
CC       a measure of protein-protein interactions. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Fluorescence excitation of the Ser-142 mutant is at 589
CC       nm and emission at 615 nm.
CC   -!- SIMILARITY: Belongs to the GFP family. {ECO:0000305}.
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DR   EMBL; AF383156; AAL27542.1; -; mRNA.
DR   AlphaFoldDB; Q95P04; -.
DR   SMR; Q95P04; -.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.155.10; -; 1.
DR   InterPro; IPR009017; GFP.
DR   InterPro; IPR011584; GFP-related.
DR   Pfam; PF01353; GFP; 1.
DR   SUPFAM; SSF54511; SSF54511; 1.
PE   1: Evidence at protein level;
KW   Chromophore; Luminescence; Photoprotein.
FT   CHAIN           1..221
FT                   /note="GFP-like non-fluorescent chromoprotein"
FT                   /id="PRO_0000192585"
FT   MOD_RES         63
FT                   /note="2,3-didehydrotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P83690"
FT   CROSSLNK        62..64
FT                   /note="2-iminomethyl-5-imidazolinone (Gln-Gly)"
FT                   /evidence="ECO:0000250|UniProtKB:P83690"
FT   MUTAGEN         142
FT                   /note="N->S: Produces a fluorescent form."
FT                   /evidence="ECO:0000269|PubMed:11682051"
SQ   SEQUENCE   221 AA;  24918 MW;  93F9F4B5C2003CB4 CRC64;
     MSVIAKQMTY KVYMSGTVNG HYFEVQGDGK GKPYEGEQTV KLTVTKGGPL PFAWDILSPQ
     SQYGSIPFTK YPEDIPDYVK QSFPEGYTWE RIMNFEDGAV CTVSNDSSIQ GNCFIYNVKF
     SGLNFPPNGP VMQKKTQGWE PNTERLFARD GMLIGNNFMA LKLEGGGHYL CEFKSTYKAK
     KPVKMPGYHY VDRKLDVTNH NIDYTSVEQC EISIARKPVV A
 
 
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