NFCP_GONTE
ID NFCP_GONTE Reviewed; 221 AA.
AC Q95P04;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=GFP-like non-fluorescent chromoprotein;
DE Short=gtCP;
OS Goniopora tenuidens (Anemone coral) (Rhodaraea tenuidens).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Scleractinia;
OC Fungiina; Poritidae; Goniopora.
OX NCBI_TaxID=75301;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAL27542.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND MUTAGENESIS OF ASN-142.
RX PubMed=11682051; DOI=10.1016/s0014-5793(01)02930-1;
RA Gurskaya N.G., Fradkov A.F., Terskikh A., Matz M.V., Labas Y.A.,
RA Martynov V.I., Yanushevich Y.G., Lukyanov K.A., Lukyanov S.A.;
RT "GFP-like chromoproteins as a source of far-red fluorescent proteins.";
RL FEBS Lett. 507:16-20(2001).
CC -!- FUNCTION: Non-fluorescent pigment protein that is lilac in color.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=580 nm;
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11682051}.
CC -!- PTM: Contains a chromophore consisting of modified amino acid residues.
CC The chromophore is formed by autocatalytic backbone condensation
CC between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to
CC didehydrotyrosine, and formation of a double bond to the alpha-amino
CC nitrogen of residue Xaa-N. Maturation of the chromophore requires
CC nothing other than molecular oxygen. The precise stereochemistry of the
CC tyrosine has not been determined.
CC -!- BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitous
CC tool for making chimeric proteins, where they function as a fluorescent
CC protein tag. Typically they tolerate N- and C-terminal fusion to a
CC broad variety of proteins. They have been expressed in most known cell
CC types and are used as a noninvasive fluorescent marker in living cells
CC and organisms. They enable a wide range of applications where they have
CC functioned as a cell lineage tracer, reporter of gene expression, or as
CC a measure of protein-protein interactions. {ECO:0000305}.
CC -!- MISCELLANEOUS: Fluorescence excitation of the Ser-142 mutant is at 589
CC nm and emission at 615 nm.
CC -!- SIMILARITY: Belongs to the GFP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF383156; AAL27542.1; -; mRNA.
DR AlphaFoldDB; Q95P04; -.
DR SMR; Q95P04; -.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR Gene3D; 2.40.155.10; -; 1.
DR InterPro; IPR009017; GFP.
DR InterPro; IPR011584; GFP-related.
DR Pfam; PF01353; GFP; 1.
DR SUPFAM; SSF54511; SSF54511; 1.
PE 1: Evidence at protein level;
KW Chromophore; Luminescence; Photoprotein.
FT CHAIN 1..221
FT /note="GFP-like non-fluorescent chromoprotein"
FT /id="PRO_0000192585"
FT MOD_RES 63
FT /note="2,3-didehydrotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P83690"
FT CROSSLNK 62..64
FT /note="2-iminomethyl-5-imidazolinone (Gln-Gly)"
FT /evidence="ECO:0000250|UniProtKB:P83690"
FT MUTAGEN 142
FT /note="N->S: Produces a fluorescent form."
FT /evidence="ECO:0000269|PubMed:11682051"
SQ SEQUENCE 221 AA; 24918 MW; 93F9F4B5C2003CB4 CRC64;
MSVIAKQMTY KVYMSGTVNG HYFEVQGDGK GKPYEGEQTV KLTVTKGGPL PFAWDILSPQ
SQYGSIPFTK YPEDIPDYVK QSFPEGYTWE RIMNFEDGAV CTVSNDSSIQ GNCFIYNVKF
SGLNFPPNGP VMQKKTQGWE PNTERLFARD GMLIGNNFMA LKLEGGGHYL CEFKSTYKAK
KPVKMPGYHY VDRKLDVTNH NIDYTSVEQC EISIARKPVV A