NFCP_HETCR
ID NFCP_HETCR Reviewed; 227 AA.
AC Q95W85;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=GFP-like non-fluorescent chromoprotein;
DE AltName: Full=HcRed;
DE AltName: Full=hcCP;
OS Heteractis crispa (Leathery sea anemone) (Radianthus macrodactylus).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Stichodactylidae; Heteractis.
OX NCBI_TaxID=175771;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAL27538.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND MUTAGENESIS OF ALA-2; THR-36;
RP LEU-122; CYS-143; LEU-173; PRO-201 AND LYS-204.
RX PubMed=11682051; DOI=10.1016/s0014-5793(01)02930-1;
RA Gurskaya N.G., Fradkov A.F., Terskikh A., Matz M.V., Labas Y.A.,
RA Martynov V.I., Yanushevich Y.G., Lukyanov K.A., Lukyanov S.A.;
RT "GFP-like chromoproteins as a source of far-red fluorescent proteins.";
RL FEBS Lett. 507:16-20(2001).
CC -!- FUNCTION: Non-fluorescent pigment protein that is lilac in color.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=578 nm;
CC Note=Fluorescence excitation of HcRed mutant is at 592 nm and
CC emission at 645 nm.;
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11682051}.
CC -!- PTM: Contains a chromophore consisting of modified amino acid residues.
CC The chromophore is formed by autocatalytic backbone condensation
CC between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to
CC didehydrotyrosine, and formation of a double bond to the alpha-amino
CC nitrogen of residue Xaa-N. Maturation of the chromophore requires
CC nothing other than molecular oxygen. The precise stereochemistry of the
CC tyrosine has not been determined.
CC -!- BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitous
CC tool for making chimeric proteins, where they function as a fluorescent
CC protein tag. Typically they tolerate N- and C-terminal fusion to a
CC broad variety of proteins. They have been expressed in most known cell
CC types and are used as a noninvasive fluorescent marker in living cells
CC and organisms. They enable a wide range of applications where they have
CC functioned as a cell lineage tracer, reporter of gene expression, or as
CC a measure of protein-protein interactions. {ECO:0000305}.
CC -!- MISCELLANEOUS: In the wild-type form, the chromophore matures at 20
CC degrees Celsius. Mutants have been selected to mature at 37 degrees
CC Celsius to make them suitable for use in vivo and cell culture.
CC -!- SIMILARITY: Belongs to the GFP family. {ECO:0000305}.
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DR EMBL; AF363776; AAL27538.1; -; mRNA.
DR PDB; 1YZW; X-ray; 2.10 A; A/B/C/D=1-227.
DR PDB; 6DEJ; X-ray; 1.63 A; A/B/C/D=1-227.
DR PDB; 6Y1G; X-ray; 2.30 A; A/B/C/D=2-227.
DR PDBsum; 1YZW; -.
DR PDBsum; 6DEJ; -.
DR PDBsum; 6Y1G; -.
DR AlphaFoldDB; Q95W85; -.
DR SMR; Q95W85; -.
DR EvolutionaryTrace; Q95W85; -.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR Gene3D; 2.40.155.10; -; 1.
DR InterPro; IPR009017; GFP.
DR InterPro; IPR011584; GFP-related.
DR Pfam; PF01353; GFP; 1.
DR SUPFAM; SSF54511; SSF54511; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromophore; Luminescence; Photoprotein.
FT CHAIN 1..227
FT /note="GFP-like non-fluorescent chromoprotein"
FT /id="PRO_0000192587"
FT MOD_RES 64
FT /note="2,3-didehydrotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P83690"
FT CROSSLNK 63..65
FT /note="2-iminomethyl-5-imidazolinone (Glu-Gly)"
FT /evidence="ECO:0000250|UniProtKB:P83690"
FT MUTAGEN 2
FT /note="A->S: In Hcred; matures at 37 degrees Celsius and
FT produces over 6-fold brighter fluorescence and a
FT homodimeric form; when associated with A-36; S-143; H-173;
FT L-201 and E-204."
FT /evidence="ECO:0000269|PubMed:11682051"
FT MUTAGEN 36
FT /note="T->A: In Hcred; matures at 37 degrees Celsius and
FT produces over 6-fold brighter fluorescence and a
FT homodimeric form; when associated with S-2; S-143; H-173;
FT L-201 and E-204."
FT /evidence="ECO:0000269|PubMed:11682051"
FT MUTAGEN 122
FT /note="L->H: Produces a dimeric form."
FT /evidence="ECO:0000269|PubMed:11682051"
FT MUTAGEN 143
FT /note="C->S: In Hcred; matures at 37 degrees Celsius and
FT produces over 6-fold brighter fluorescence and a
FT homodimeric form; when associated with S-2; A-36; H-173; L-
FT 201 and E-204."
FT /evidence="ECO:0000269|PubMed:11682051"
FT MUTAGEN 173
FT /note="L->H: In Hcred; matures at 37 degrees Celsius and
FT produces over 6-fold brighter fluorescence and a
FT homodimeric form; when associated with S-2; A-36; S-143; L-
FT 201 and E-204."
FT /evidence="ECO:0000269|PubMed:11682051"
FT MUTAGEN 201
FT /note="P->L: In Hcred; matures at 37 degrees Celsius and
FT produces over 6-fold brighter fluorescence and a
FT homodimeric form; when associated with S-2; A-36; S-143; H-
FT 173 and E-204."
FT /evidence="ECO:0000269|PubMed:11682051"
FT MUTAGEN 204
FT /note="K->E: In Hcred; matures at 37 degrees Celsius and
FT produces over 6-fold brighter fluorescence and a
FT homodimeric form; when associated with S-2; A-36; S-143; H-
FT 173 and L-201."
FT /evidence="ECO:0000269|PubMed:11682051"
FT STRAND 7..19
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 22..33
FT /evidence="ECO:0007829|PDB:6DEJ"
FT TURN 34..37
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 38..48
FT /evidence="ECO:0007829|PDB:6DEJ"
FT HELIX 55..57
FT /evidence="ECO:0007829|PDB:6DEJ"
FT HELIX 59..61
FT /evidence="ECO:0007829|PDB:6DEJ"
FT HELIX 79..82
FT /evidence="ECO:0007829|PDB:6DEJ"
FT TURN 83..86
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 88..96
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 101..111
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 114..124
FT /evidence="ECO:0007829|PDB:6DEJ"
FT TURN 131..135
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 143..150
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 153..164
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 167..181
FT /evidence="ECO:0007829|PDB:6DEJ"
FT HELIX 183..185
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 191..204
FT /evidence="ECO:0007829|PDB:6DEJ"
FT TURN 205..207
FT /evidence="ECO:0007829|PDB:6DEJ"
FT STRAND 208..218
FT /evidence="ECO:0007829|PDB:6DEJ"
SQ SEQUENCE 227 AA; 25637 MW; CB40899E95E7EC64 CRC64;
MAGLLKESMR IKMYMEGTVN GHYFKCEGEG DGNPFTGTQS MRIHVTEGAP LPFAFDILAP
CCEYGSRTFV HHTAEIPDFF KQSFPEGFTW ERTTTYEDGG ILTAHQDTSL EGNCLIYKVK
VLGTNFPADG PVMKNKSGGW EPCTEVVYPE NGVLCGRNVM ALKVGDRRLI CHLYTSYRSK
KAVRALTMPG FHFTDIRLQM PRKKKDEYFE LYEASVARYS DLPEKAN
