NFD2_ARATH
ID NFD2_ARATH Reviewed; 191 AA.
AC Q9FYL8;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Protein NUCLEAR FUSION DEFECTIVE 2 {ECO:0000303|PubMed:16698901};
DE Flags: Precursor;
GN Name=NFD2 {ECO:0000303|PubMed:16698901};
GN OrderedLocusNames=At1g24450 {ECO:0000312|Araport:AT1G24450};
GN ORFNames=F21J9.11 {ECO:0000312|EMBL:AAF97957.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16698901; DOI=10.1104/pp.106.079319;
RA Portereiko M.F., Sandaklie-Nikolova L., Lloyd A., Dever C.A., Otsuga D.,
RA Drews G.N.;
RT "NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is
RT required for karyogamy during female gametophyte development and
RT fertilization.";
RL Plant Physiol. 141:957-965(2006).
CC -!- FUNCTION: Required for karyogamy during female gametophyte development,
CC when the two polar nuclei fuse to form the diploid central cell
CC nucleus. {ECO:0000269|PubMed:16698901}.
CC -!- DISRUPTION PHENOTYPE: Failure of fusion of the polar nuclei during
CC megagametogenesis. {ECO:0000269|PubMed:16698901}.
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DR EMBL; AC000103; AAF97957.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30531.1; -; Genomic_DNA.
DR EMBL; AY045598; AAK73956.1; -; mRNA.
DR EMBL; AY094027; AAM16183.1; -; mRNA.
DR RefSeq; NP_173854.1; NM_102290.4.
DR AlphaFoldDB; Q9FYL8; -.
DR SMR; Q9FYL8; -.
DR iPTMnet; Q9FYL8; -.
DR PaxDb; Q9FYL8; -.
DR PRIDE; Q9FYL8; -.
DR ProteomicsDB; 251109; -.
DR EnsemblPlants; AT1G24450.1; AT1G24450.1; AT1G24450.
DR GeneID; 839061; -.
DR Gramene; AT1G24450.1; AT1G24450.1; AT1G24450.
DR KEGG; ath:AT1G24450; -.
DR Araport; AT1G24450; -.
DR TAIR; locus:2024011; AT1G24450.
DR eggNOG; ENOG502RXH7; Eukaryota.
DR HOGENOM; CLU_126823_0_0_1; -.
DR InParanoid; Q9FYL8; -.
DR OMA; DAGNVFW; -.
DR OrthoDB; 1375361at2759; -.
DR PhylomeDB; Q9FYL8; -.
DR PRO; PR:Q9FYL8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FYL8; baseline and differential.
DR Genevisible; Q9FYL8; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0003725; F:double-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR GO; GO:0000741; P:karyogamy; IMP:UniProtKB.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR Gene3D; 1.10.1520.10; -; 1.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR Pfam; PF14622; Ribonucleas_3_3; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; SSF69065; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Karyogamy; Reference proteome; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..191
FT /note="Protein NUCLEAR FUSION DEFECTIVE 2"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431841"
FT DOMAIN 48..167
FT /note="RNase III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00177"
SQ SEQUENCE 191 AA; 20742 MW; 3B3182038836B9FC CRC64;
MATLRFTLLL LVFVVGIFFS FSSVSHVRAT SEINLRIEPF SSPFATDLAK LQTQIGYKFN
NINLLRRAMT HASFSQENNK ALSIFGTHII ETAVSLQFLA KDIDISSKAL GRLISEVSNV
ESSCALDGDR LGLGKIIRVS TKTDASNSAI LCTGFRAIFG AIAIDAGTVD EAIKVFWKVH
GARAGRLVSM L
