NFD4_ARATH
ID NFD4_ARATH Reviewed; 582 AA.
AC F4I9E1; Q9C862;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Protein NUCLEAR FUSION DEFECTIVE 4 {ECO:0000303|PubMed:16698901};
GN Name=NFD4 {ECO:0000303|PubMed:16698901};
GN OrderedLocusNames=At1g31470 {ECO:0000312|Araport:AT1G31470};
GN ORFNames=T8E3.15 {ECO:0000312|EMBL:AAG51261.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16698901; DOI=10.1104/pp.106.079319;
RA Portereiko M.F., Sandaklie-Nikolova L., Lloyd A., Dever C.A., Otsuga D.,
RA Drews G.N.;
RT "NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is
RT required for karyogamy during female gametophyte development and
RT fertilization.";
RL Plant Physiol. 141:957-965(2006).
CC -!- FUNCTION: Required for karyogamy during female gametophyte development,
CC when the two polar nuclei fuse to form the diploid central cell nucleus
CC (PubMed:16698901). {ECO:0000269|PubMed:16698901}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Failure of fusion of the polar nuclei during
CC megagametogenesis. {ECO:0000269|PubMed:16698901}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51261.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC027135; AAG51261.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE31359.1; -; Genomic_DNA.
DR PIR; G86440; G86440.
DR RefSeq; NP_174432.2; NM_102886.2.
DR AlphaFoldDB; F4I9E1; -.
DR STRING; 3702.AT1G31470.1; -.
DR iPTMnet; F4I9E1; -.
DR PaxDb; F4I9E1; -.
DR PRIDE; F4I9E1; -.
DR ProteomicsDB; 249380; -.
DR EnsemblPlants; AT1G31470.1; AT1G31470.1; AT1G31470.
DR GeneID; 840037; -.
DR Gramene; AT1G31470.1; AT1G31470.1; AT1G31470.
DR KEGG; ath:AT1G31470; -.
DR Araport; AT1G31470; -.
DR TAIR; locus:2206169; AT1G31470.
DR eggNOG; ENOG502QVB4; Eukaryota.
DR HOGENOM; CLU_021715_2_0_1; -.
DR InParanoid; F4I9E1; -.
DR OMA; ISRLEFW; -.
DR OrthoDB; 458757at2759; -.
DR PRO; PR:F4I9E1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I9E1; baseline and differential.
DR Genevisible; F4I9E1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; ISM:TAIR.
DR GO; GO:0000741; P:karyogamy; IMP:UniProtKB.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR010658; Nodulin-like.
DR Pfam; PF06813; Nodulin-like; 1.
DR SUPFAM; SSF103473; SSF103473; 2.
PE 3: Inferred from homology;
KW Developmental protein; Glycoprotein; Karyogamy; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..582
FT /note="Protein NUCLEAR FUSION DEFECTIVE 4"
FT /id="PRO_0000431843"
FT TRANSMEM 46..66
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..412
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TRANSMEM 536..556
FT /note="Helical; Name=13"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 582 AA; 64060 MW; 3DA872C559260FB0 CRC64;
MRPRIRDVSD KLRPNRASFD DGETKFHRKH LPPLRTMFGR WRKWTVLVAA IWIQASTGTN
FDFSAYSSHL KSVLGISQVR LNYLAVASDL GKAFGWSSGI ALGYFPLSVV LFAAAAMGFV
GYGVQWLVIT NIITLPYSLV FLCCLLAGLS ICWFNTACFI LCIRHFPNNR ALALSLTVSF
NGISAALYSL AFNAINPSSS NLYLLLNSLV PLVVSFAALY PVLTKPSLDT TPDYDSRRHD
SHVFTILNVL AVITSFHLLL SSSSTSSARL NFIGAVVLLV FPLCAPLLVY ARDYFLPVIN
ARLNHESSGY VMLNIDELKN QKTSVSSKTG YEHMGTAKEG NTVRLGDEHS FRLLISRLEF
WLYYIAYFCG GTIGLVYSNN LGQIAQSLGQ NSTTLVTIYS SFSFFGRLLS AAPDFMHKRF
RLTRTGWFAI ALLPTPIAFF LLAVSSSQQT ALQTATALIG LSSGFIFAAA VSITSDLFGP
NSVGVNHNIL ITNIPIGSLL YGYIAASIYE ANASPDITPI VSDSIVCIGR DCYFKTFVFW
GCLSILGVVS SLSLYIRTKP VYHRLEQDKV SLTSSYKDLD PL
