NFD5_ARATH
ID NFD5_ARATH Reviewed; 372 AA.
AC Q8LEZ4; Q9LN43;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Protein NUCLEAR FUSION DEFECTIVE 5, mitochondrial {ECO:0000303|PubMed:16698901};
DE Flags: Precursor;
GN Name=NFD5 {ECO:0000303|PubMed:16698901};
GN OrderedLocusNames=At1g19520 {ECO:0000312|Araport:AT1G19520};
GN ORFNames=F18O14.40 {ECO:0000312|EMBL:AAF79432.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16698901; DOI=10.1104/pp.106.079319;
RA Portereiko M.F., Sandaklie-Nikolova L., Lloyd A., Dever C.A., Otsuga D.,
RA Drews G.N.;
RT "NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is
RT required for karyogamy during female gametophyte development and
RT fertilization.";
RL Plant Physiol. 141:957-965(2006).
CC -!- FUNCTION: Required for karyogamy during female gametophyte development,
CC when the two polar nuclei fuse to form the diploid central cell
CC nucleus. {ECO:0000269|PubMed:16698901}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8LEZ4-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Failure of fusion of the polar nuclei during
CC megagametogenesis. {ECO:0000269|PubMed:16698901}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79432.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g19520 and At1g19525.; Evidence={ECO:0000305};
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DR EMBL; AC025808; AAF79432.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29864.1; -; Genomic_DNA.
DR EMBL; AY085135; AAM61688.1; -; mRNA.
DR EMBL; BT025250; ABF19003.1; -; mRNA.
DR PIR; A86328; A86328.
DR RefSeq; NP_001185038.1; NM_001198109.2. [Q8LEZ4-1]
DR AlphaFoldDB; Q8LEZ4; -.
DR SMR; Q8LEZ4; -.
DR BioGRID; 23777; 7.
DR IntAct; Q8LEZ4; 1.
DR STRING; 3702.AT1G19520.1; -.
DR PaxDb; Q8LEZ4; -.
DR PRIDE; Q8LEZ4; -.
DR ProteomicsDB; 236822; -. [Q8LEZ4-1]
DR EnsemblPlants; AT1G19520.2; AT1G19520.2; AT1G19520. [Q8LEZ4-1]
DR GeneID; 838538; -.
DR Gramene; AT1G19520.2; AT1G19520.2; AT1G19520. [Q8LEZ4-1]
DR KEGG; ath:AT1G19520; -.
DR Araport; AT1G19520; -.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_757318_0_0_1; -.
DR InParanoid; Q8LEZ4; -.
DR PhylomeDB; Q8LEZ4; -.
DR PRO; PR:Q8LEZ4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LEZ4; baseline and differential.
DR Genevisible; Q8LEZ4; AT.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0000741; P:karyogamy; IMP:UniProtKB.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:UniProtKB.
DR InterPro; IPR044657; NFD5-like.
DR PANTHER; PTHR46862; PTHR46862; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Karyogamy; Mitochondrion;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..28
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 29..372
FT /note="Protein NUCLEAR FUSION DEFECTIVE 5, mitochondrial"
FT /id="PRO_0000342699"
FT REGION 240..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 372 AA; 43110 MW; DA8E9CF563C4A348 CRC64;
MKSFLLSRQA IHRISLLSSK TPTFCRNFSA ITSTISHSDR HLRSYDEQTP FQNVEIPRPI
SSFNRYFHFT RESRLSESSA AIDDSNDQEE DDEDGTTNEF LSRFVWIMRG KVSEAYPDCD
KKMIDGMLLL IVEKVVEEIE RGGFNKVGSA PPSPSSEFSD DLWATIWEVS NTVLKDMEKE
RKKEKMKQYV QSPEVMEMCR FAGEIGIRGD LLRELRFKWA REKMDDAEFY ESLEQQRDLD
NSIRESETVD GEVEEEGFVP SDEVESRSIS LPKRKGKLKY KIYGLELSDP KWVEMADKIH
EAEEEADWRE PKPVTGKCKL VMEKLESLQE GDDPSGLLAE WAELLEPNRV DWIALINQLR
EGNTHAYLKV RL
