NFD6_ARATH
ID NFD6_ARATH Reviewed; 100 AA.
AC Q93ZJ3; B3H5P1; F4IVG2;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial {ECO:0000303|PubMed:16698901};
DE Flags: Precursor;
GN Name=NFD6 {ECO:0000303|PubMed:16698901};
GN OrderedLocusNames=At2g20585 {ECO:0000312|Araport:AT2G20585};
GN ORFNames=F23N11 {ECO:0000312|EMBL:AAM15363.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL09736.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16698901; DOI=10.1104/pp.106.079319;
RA Portereiko M.F., Sandaklie-Nikolova L., Lloyd A., Dever C.A., Otsuga D.,
RA Drews G.N.;
RT "NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is
RT required for karyogamy during female gametophyte development and
RT fertilization.";
RL Plant Physiol. 141:957-965(2006).
CC -!- FUNCTION: Required for karyogamy during female gametophyte development,
CC when the two polar nuclei fuse to form the diploid central cell
CC nucleus. {ECO:0000269|PubMed:16698901}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q6NNL9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q93ZJ3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93ZJ3-2; Sequence=VSP_057448;
CC Name=3;
CC IsoId=Q93ZJ3-3; Sequence=VSP_057447;
CC -!- DISRUPTION PHENOTYPE: Failure of fusion of the polar nuclei during
CC megagametogenesis. {ECO:0000269|PubMed:16698901}.
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DR EMBL; AC007048; AAM15363.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07033.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07034.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07035.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62509.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62510.1; -; Genomic_DNA.
DR EMBL; AY057495; AAL09736.1; -; mRNA.
DR EMBL; AY103298; AAM65350.1; -; mRNA.
DR EMBL; AY085547; AAM62771.1; -; mRNA.
DR RefSeq; NP_001118352.1; NM_001124880.2. [Q93ZJ3-3]
DR RefSeq; NP_001324661.1; NM_001335696.1. [Q93ZJ3-1]
DR RefSeq; NP_001324662.1; NM_001335695.1. [Q93ZJ3-3]
DR RefSeq; NP_565478.1; NM_127619.4. [Q93ZJ3-1]
DR RefSeq; NP_850002.1; NM_179671.4. [Q93ZJ3-2]
DR AlphaFoldDB; Q93ZJ3; -.
DR IntAct; Q93ZJ3; 3.
DR STRING; 3702.AT2G20585.1; -.
DR PaxDb; Q93ZJ3; -.
DR ProteomicsDB; 251158; -. [Q93ZJ3-1]
DR EnsemblPlants; AT2G20585.1; AT2G20585.1; AT2G20585. [Q93ZJ3-1]
DR EnsemblPlants; AT2G20585.2; AT2G20585.2; AT2G20585. [Q93ZJ3-2]
DR EnsemblPlants; AT2G20585.3; AT2G20585.3; AT2G20585. [Q93ZJ3-3]
DR EnsemblPlants; AT2G20585.4; AT2G20585.4; AT2G20585. [Q93ZJ3-3]
DR EnsemblPlants; AT2G20585.5; AT2G20585.5; AT2G20585. [Q93ZJ3-1]
DR GeneID; 816581; -.
DR Gramene; AT2G20585.1; AT2G20585.1; AT2G20585. [Q93ZJ3-1]
DR Gramene; AT2G20585.2; AT2G20585.2; AT2G20585. [Q93ZJ3-2]
DR Gramene; AT2G20585.3; AT2G20585.3; AT2G20585. [Q93ZJ3-3]
DR Gramene; AT2G20585.4; AT2G20585.4; AT2G20585. [Q93ZJ3-3]
DR Gramene; AT2G20585.5; AT2G20585.5; AT2G20585. [Q93ZJ3-1]
DR KEGG; ath:AT2G20585; -.
DR Araport; AT2G20585; -.
DR TAIR; locus:505006258; AT2G20585.
DR eggNOG; ENOG502S3KJ; Eukaryota.
DR OMA; CLETLMP; -.
DR PhylomeDB; Q93ZJ3; -.
DR PRO; PR:Q93ZJ3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q93ZJ3; baseline and differential.
DR Genevisible; Q93ZJ3; AT.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0000741; P:karyogamy; IMP:UniProtKB.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:UniProtKB.
DR InterPro; IPR043459; NFD6/NOXY2-like.
DR PANTHER; PTHR33156; PTHR33156; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Developmental protein; Karyogamy; Mitochondrion;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..29
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 30..100
FT /note="Protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431844"
FT VAR_SEQ 95..100
FT /note="ACNDDV -> G (in isoform 3)"
FT /id="VSP_057447"
FT VAR_SEQ 97..100
FT /note="NDDV -> KEDF (in isoform 2)"
FT /id="VSP_057448"
SQ SEQUENCE 100 AA; 10451 MW; 7B12286D8FBE638B CRC64;
MATSAVLSGA RSMLRAASSR SAAASTGRFA SQAKSAPPLF RATARRSPLL SPLRNPVELS
FCVESLLPYH SATASALMTS KLSISGQTYG WLSDACNDDV
