NFI1_CAEEL
ID NFI1_CAEEL Reviewed; 823 AA.
AC Q5H9N3; Q09631; Q5H9N2;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Nuclear factor I family protein {ECO:0000312|WormBase:ZK1290.4c};
GN Name=nfi-1 {ECO:0000312|WormBase:ZK1290.4c};
GN ORFNames=ZK1290.4 {ECO:0000312|WormBase:ZK1290.4c};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16242019; DOI=10.1186/1471-213x-5-24;
RA Lazakovitch E., Kalb J.M., Matsumoto R., Hirono K., Kohara Y.,
RA Gronostajski R.M.;
RT "nfi-I affects behavior and life-span in C. elegans but is not essential
RT for DNA replication or survival.";
RL BMC Dev. Biol. 5:24-24(2005).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18651662; DOI=10.1002/dvdy.21632;
RA Lazakovitch E., Kalb J.M., Gronostajski R.M.;
RT "Lifespan extension and increased pumping rate accompany pharyngeal muscle-
RT specific expression of nfi-1 in C. elegans.";
RL Dev. Dyn. 237:2100-2107(2008).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=19584245; DOI=10.1073/pnas.0812894106;
RA Whittle C.M., Lazakovitch E., Gronostajski R.M., Lieb J.D.;
RT "DNA-binding specificity and in vivo targets of Caenorhabditis elegans
RT nuclear factor I.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:12049-12054(2009).
CC -!- FUNCTION: Probable transcription factor which recognizes and binds the
CC palindromic sequence 5'-TTGGCANNNTGCCAA-3' present in promoters
CC (PubMed:16242019, PubMed:19584245). Plays a role in locomotion,
CC pharyngeal pumping, egg-laying, and life span (PubMed:16242019,
CC PubMed:18651662, PubMed:19584245). {ECO:0000269|PubMed:16242019,
CC ECO:0000269|PubMed:18651662, ECO:0000269|PubMed:19584245}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00436,
CC ECO:0000269|PubMed:18651662}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=c {ECO:0000312|WormBase:ZK1290.4c};
CC IsoId=Q5H9N3-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:ZK1290.4b};
CC IsoId=Q5H9N3-2; Sequence=VSP_061000;
CC Name=a {ECO:0000312|WormBase:ZK1290.4a};
CC IsoId=Q5H9N3-3; Sequence=VSP_061001;
CC -!- TISSUE SPECIFICITY: Expressed in muscles, neurons and intestinal cells.
CC {ECO:0000269|PubMed:16242019, ECO:0000269|PubMed:18651662}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally in 2-cell embryos,
CC zygotically in early and mid-embryogenesis; expression decreases after
CC gastrulation and is abolished in L1 larvae, but re-expressed in the
CC adult gonad. {ECO:0000269|PubMed:16242019}.
CC -!- DISRUPTION PHENOTYPE: Defects in locomotion, pharyngeal pumping rate,
CC egg-laying, and reduced life-span. {ECO:0000269|PubMed:16242019,
CC ECO:0000269|PubMed:18651662}.
CC -!- SIMILARITY: Belongs to the CTF/NF-I family. {ECO:0000255|PROSITE-
CC ProRule:PRU00436}.
CC -!- CAUTION: Apparent discrepancy in expression data: a transgenic promoter
CC based on about 4 kb from the 5' flank of the gene, drives reporter gene
CC expression in larval L1-L4 stages, but endogenous transcripts are not
CC detected in L1 larvae using in-situ hybridisation.
CC {ECO:0000269|PubMed:16242019}.
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DR EMBL; BX284602; CCD65933.1; -; Genomic_DNA.
DR EMBL; BX284602; CCD65934.1; -; Genomic_DNA.
DR EMBL; BX284602; CCD65935.1; -; Genomic_DNA.
DR RefSeq; NP_001022505.1; NM_001027334.2. [Q5H9N3-3]
DR RefSeq; NP_001022506.1; NM_001027335.3.
DR RefSeq; NP_001022507.1; NM_001027336.3.
DR AlphaFoldDB; Q5H9N3; -.
DR DIP; DIP-27009N; -.
DR IntAct; Q5H9N3; 12.
DR STRING; 6239.ZK1290.4c; -.
DR PaxDb; Q5H9N3; -.
DR EnsemblMetazoa; ZK1290.4a.1; ZK1290.4a.1; WBGene00003592. [Q5H9N3-3]
DR EnsemblMetazoa; ZK1290.4b.1; ZK1290.4b.1; WBGene00003592. [Q5H9N3-2]
DR EnsemblMetazoa; ZK1290.4b.2; ZK1290.4b.2; WBGene00003592. [Q5H9N3-2]
DR EnsemblMetazoa; ZK1290.4b.3; ZK1290.4b.3; WBGene00003592. [Q5H9N3-2]
DR EnsemblMetazoa; ZK1290.4c.1; ZK1290.4c.1; WBGene00003592. [Q5H9N3-1]
DR GeneID; 174225; -.
DR UCSC; ZK1290.4c; c. elegans.
DR CTD; 174225; -.
DR WormBase; ZK1290.4a; CE37597; WBGene00003592; nfi-1.
DR WormBase; ZK1290.4b; CE38016; WBGene00003592; nfi-1.
DR WormBase; ZK1290.4c; CE38017; WBGene00003592; nfi-1.
DR eggNOG; KOG3663; Eukaryota.
DR GeneTree; ENSGT00950000182916; -.
DR HOGENOM; CLU_014774_0_0_1; -.
DR InParanoid; Q5H9N3; -.
DR OMA; QVVIFRK; -.
DR OrthoDB; 815693at2759; -.
DR SignaLink; Q5H9N3; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003592; Expressed in germ line (C elegans) and 5 other tissues.
DR ExpressionAtlas; Q5H9N3; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0040012; P:regulation of locomotion; IMP:WormBase.
DR GO; GO:0046662; P:regulation of oviposition; IMP:WormBase.
DR GO; GO:0043051; P:regulation of pharyngeal pumping; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR000647; CTF/NFI.
DR InterPro; IPR020604; CTF/NFI_DNA-bd-dom.
DR InterPro; IPR019548; CTF/NFI_DNA-bd_N.
DR InterPro; IPR003619; MAD_homology1_Dwarfin-type.
DR PANTHER; PTHR11492; PTHR11492; 1.
DR Pfam; PF03165; MH1; 1.
DR Pfam; PF10524; NfI_DNAbd_pre-N; 1.
DR SMART; SM00523; DWA; 1.
DR PROSITE; PS51080; CTF_NFI_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA replication; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..823
FT /note="Nuclear factor I family protein"
FT /id="PRO_0000452408"
FT DNA_BIND 61..253
FT /note="CTF/NF-I"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00436"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..408
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 433..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 777..823
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..54
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..381
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 382..396
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 433..464
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 779..808
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..36
FT /note="Missing (in isoform b)"
FT /id="VSP_061000"
FT VAR_SEQ 54..69
FT /note="Missing (in isoform a)"
FT /id="VSP_061001"
SQ SEQUENCE 823 AA; 89569 MW; AE0EB00EBDAD52EB CRC64;
MEPHLKIDVS SASGSTTTGA TASTSEAPQD SQAQQTMPPP SSDWSNQFNS PEAVSPKANG
IKCFSPYSQE DMGPFVEQLL PFVRASAYNW FHLQAAKRRH FKEFDKKMCA SEENAKLAEL
QNDRDELKVK WASRLLGKIK KDIQNDDKEA FISAINGSEP NKCIISVADQ KGKMRRIDCL
RQADKVWRLD LVTIILFKGI PLESTDGERL ERSEACVHPL CINPFHMAIS VRGLDVFMAN
YLKDVDTKIT LTYPRNDELS DTVMVKQEPG EQTIVAPHAV LGTSSTHTRV WETNSERNAE
TIIITYDPQK ACHTYFGGRA TLAQQSLSAG NTYMVNKTAV DNNFFNAKRS VLCLPPPPIQ
NCFPYPIAGT SDSQQMDMSE DSNDGPSEKR SRDISSHDSP NSSTNDEVRR IVESGTEKLV
LGSSIWAAPG QFSRTQQNQG APGTSRQVRP LPDFQSQDSA RSPGAFRSTA KPVCRMTVNT
GNHGDVGVVV VDERNREHVI HAQHIVNALS SLRTTPSMRE SPVGRKRMHP HTSNSFEFLN
CNQEMNKNEG ALGSDISPTH TAVSNLISRE SSGYMASPTK FTTARGDTTS FSKIFQKIEE
KHLQHNQPST SYCNSQIQPP ILSSKPVDSS VKLIAPVAVK PIMSGCNSII PSPITTPRIT
PSFRMLEDDS LINVLGQLAH SNDGTTLNDS FIQHLIDTNS RSPLLSSGNA FSALSMGAVS
GLVPGNSIHR PDSSASNGSN SLGVAMGLAV PQNIALAVQQ TQNAMSPLHQ IRVSVGAPPA
CSPSSSNSSL GAANQAPVSN TPQDPNAPKL PTDFSHALRN EKK
