NFIB_CHICK
ID NFIB_CHICK Reviewed; 560 AA.
AC P17924; P17925;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Nuclear factor 1 B-type;
DE Short=NF1-B;
DE Short=Nuclear factor 1/B;
DE AltName: Full=CCAAT-box-binding transcription factor;
DE Short=CTF;
DE AltName: Full=Nuclear factor I/B;
DE Short=NF-I/B;
DE Short=NFI-B;
DE AltName: Full=TGGCA-binding protein;
GN Name=NFIB; Synonyms=NFI-B;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND PARTIAL PROTEIN
RP SEQUENCE.
RC TISSUE=Embryo;
RX PubMed=2339052; DOI=10.1093/nar/18.9.2607;
RA Rupp R.A.W., Kruse U., Multhaup G., Goebel U., Beyreuther K., Sippel A.E.;
RT "Chicken NFI/TGGCA proteins are encoded by at least three independent
RT genes: NFI-A, NFI-B and NFI-C with homologues in mammalian genomes.";
RL Nucleic Acids Res. 18:2607-2616(1990).
CC -!- FUNCTION: Recognizes and binds the palindromic sequence 5'-
CC TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the
CC origin of replication of adenovirus type 2. These proteins are
CC individually capable of activating transcription and replication.
CC -!- SUBUNIT: Binds DNA as a homodimer.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=NFI-B1;
CC IsoId=P17924-1; Sequence=Displayed;
CC Name=2; Synonyms=NFI-B2;
CC IsoId=P17924-2; Sequence=VSP_003550, VSP_003551;
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:O00712}.
CC -!- SIMILARITY: Belongs to the CTF/NF-I family. {ECO:0000255|PROSITE-
CC ProRule:PRU00436}.
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DR EMBL; X51485; CAA35852.1; -; mRNA.
DR EMBL; X51484; CAA35851.1; -; mRNA.
DR PIR; S09995; S09995.
DR RefSeq; NP_990603.1; NM_205272.2. [P17924-1]
DR RefSeq; XP_015135568.1; XM_015280082.1. [P17924-2]
DR AlphaFoldDB; P17924; -.
DR STRING; 9031.ENSGALP00000008721; -.
DR PaxDb; P17924; -.
DR Ensembl; ENSGALT00000061095; ENSGALP00000044762; ENSGALG00000005441. [P17924-1]
DR GeneID; 396209; -.
DR KEGG; gga:396209; -.
DR CTD; 4781; -.
DR VEuPathDB; HostDB:geneid_396209; -.
DR eggNOG; KOG3663; Eukaryota.
DR GeneTree; ENSGT00950000182916; -.
DR InParanoid; P17924; -.
DR PhylomeDB; P17924; -.
DR TreeFam; TF313889; -.
DR PRO; PR:P17924; -.
DR Proteomes; UP000000539; Chromosome Z.
DR Bgee; ENSGALG00000005441; Expressed in cerebellum and 11 other tissues.
DR ExpressionAtlas; P17924; baseline and differential.
DR GO; GO:0044300; C:cerebellar mossy fiber; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0021960; P:anterior commissure morphogenesis; ISS:UniProtKB.
DR GO; GO:0002062; P:chondrocyte differentiation; ISS:UniProtKB.
DR GO; GO:0060486; P:club cell differentiation; ISS:UniProtKB.
DR GO; GO:0071679; P:commissural neuron axon guidance; ISS:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0010001; P:glial cell differentiation; ISS:UniProtKB.
DR GO; GO:0061141; P:lung ciliated cell differentiation; ISS:UniProtKB.
DR GO; GO:2000795; P:negative regulation of epithelial cell proliferation involved in lung morphogenesis; ISS:UniProtKB.
DR GO; GO:2000791; P:negative regulation of mesenchymal cell proliferation involved in lung development; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0021740; P:principal sensory nucleus of trigeminal nerve development; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060509; P:type I pneumocyte differentiation; ISS:UniProtKB.
DR GO; GO:0060510; P:type II pneumocyte differentiation; ISS:UniProtKB.
DR InterPro; IPR000647; CTF/NFI.
DR InterPro; IPR020604; CTF/NFI_DNA-bd-dom.
DR InterPro; IPR019739; CTF/NFI_DNA-bd_CS.
DR InterPro; IPR019548; CTF/NFI_DNA-bd_N.
DR InterPro; IPR003619; MAD_homology1_Dwarfin-type.
DR PANTHER; PTHR11492; PTHR11492; 1.
DR Pfam; PF00859; CTF_NFI; 1.
DR Pfam; PF03165; MH1; 1.
DR Pfam; PF10524; NfI_DNAbd_pre-N; 1.
DR SMART; SM00523; DWA; 1.
DR PROSITE; PS00349; CTF_NFI_1; 1.
DR PROSITE; PS51080; CTF_NFI_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Direct protein sequencing;
KW DNA replication; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..560
FT /note="Nuclear factor 1 B-type"
FT /id="PRO_0000100198"
FT DNA_BIND 1..195
FT /note="CTF/NF-I"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00436"
FT REGION 257..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 396..404
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:O00712"
FT COMPBIAS 257..274
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 287..301
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 397..423
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 415..419
FT /note="PNSSG -> SWYLG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:2339052"
FT /id="VSP_003550"
FT VAR_SEQ 420..560
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:2339052"
FT /id="VSP_003551"
SQ SEQUENCE 560 AA; 62623 MW; 24D31C5E2651F044 CRC64;
MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE
LLSEKPEIKQ KWASRLLAKL RKDIRQEFRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC
LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL
AYYVQEQDSG QPGSPSHNDP AKNPPVYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP
IGELPSQPYY HDMNSGINLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS
RTWHERDQDM SSPTMKKPEK PLFSPTSPQD SSPRLSTFPQ HHHPGIPGVA QSVISTRTPP
SPSPLPFPAQ AILPPAPSSY FSHPTIRYPP HLNPQDTLKN YVPSYDPSSP QTSQPNSSGQ
VVGKVPGHFT PVLAPSPHHS AVRPVTLTMT DTKPITTSTE AYTASGTSQA NRYVGLSPRD
PSFLHQQQLR ICDWTMNQNG RHLYPSASED TLGITWQSPG TWASLVPFQV SNRTPILPTN
VPNYGLNIIG EPFLQAESSN
