NFIL_PIG
ID NFIL_PIG Reviewed; 231 AA.
AC P14057;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Nuclear factor 1;
DE AltName: Full=CCAAT-box-binding transcription factor;
DE Short=CTF;
DE AltName: Full=Nuclear factor I;
DE Short=NF-I;
DE AltName: Full=TGGCA-binding protein;
DE Flags: Fragment;
GN Name=NFI;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2841167; DOI=10.1016/0014-5793(88)80279-5;
RA Meisterernst M., Rogge L., Donath C., Gander I., Lottspeich F., Mertz R.,
RA Dobner T., Foeckler R., Stelzer G., Winnacker E.L.;
RT "Isolation and characterization of the porcine nuclear factor I (NFI)
RT gene.";
RL FEBS Lett. 236:27-32(1988).
RN [2]
RP PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Liver;
RX PubMed=3207762; DOI=10.1016/0167-4781(88)90114-5;
RA Gander I., Foeckler R., Rogge L., Meisterernst M., Schneider R., Mertz R.,
RA Lottspeich F., Winnacker E.L.;
RT "Purification methods for the sequence-specific DNA-binding protein nuclear
RT factor I (NFI) -- generation of protein sequence information.";
RL Biochim. Biophys. Acta 951:411-418(1988).
CC -!- FUNCTION: Recognizes and binds the palindromic sequence 5'-
CC TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the
CC origin of replication of adenovirus type 2. These proteins are
CC individually capable of activating transcription and replication.
CC -!- SUBUNIT: Binds DNA as a homodimer.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced.;
CC Name=1;
CC IsoId=P14057-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the CTF/NF-I family. {ECO:0000255|PROSITE-
CC ProRule:PRU00436}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X12764; CAA31254.1; -; Genomic_DNA.
DR PIR; JT0463; JT0463.
DR AlphaFoldDB; P14057; -.
DR PeptideAtlas; P14057; -.
DR PRIDE; P14057; -.
DR InParanoid; P14057; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProt.
DR InterPro; IPR000647; CTF/NFI.
DR InterPro; IPR020604; CTF/NFI_DNA-bd-dom.
DR InterPro; IPR019739; CTF/NFI_DNA-bd_CS.
DR InterPro; IPR019548; CTF/NFI_DNA-bd_N.
DR InterPro; IPR003619; MAD_homology1_Dwarfin-type.
DR PANTHER; PTHR11492; PTHR11492; 1.
DR Pfam; PF03165; MH1; 1.
DR Pfam; PF10524; NfI_DNAbd_pre-N; 1.
DR SMART; SM00523; DWA; 1.
DR PROSITE; PS00349; CTF_NFI_1; 1.
DR PROSITE; PS51080; CTF_NFI_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Direct protein sequencing;
KW DNA replication; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..>231
FT /note="Nuclear factor 1"
FT /id="PRO_0000100207"
FT DNA_BIND 1..192
FT /note="CTF/NF-I"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00436"
FT REGION 188..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 231
SQ SEQUENCE 231 AA; 26162 MW; A463C8BEE3D0E743 CRC64;
MGSRLTQDEF HPFIEALLPH VRAFAYTWFN LQARKRKYFK KHEKRMSKDE ERAVKDELLG
EKAEVKQKWA SRLLAKLRKD IRPECREDFV LAITGKKAPG CVLSNPDQKG KMRRIDCLRQ
ADKVWRLDLV MVILFKGIPL ESTDGERLVK AAQCGHPVLC VQPHHIGVAV KELDLYLAYF
VRERGEAGRA RGRGSDGREG MRRASQSGRP GESGPAGLRD QAGTRSLGWA G
