ID   NFL_XENLA               Reviewed;         544 AA.
AC   P35616;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Neurofilament light polypeptide;
DE            Short=NF-L;
DE   AltName: Full=Neurofilament triplet L protein;
GN   Name=nefl;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=1494944; DOI=10.1523/jneurosci.12-08-03010.1992;
RA   Charnas L.R., Szaro B.G., Gainer H.;
RT   "Identification and developmental expression of a novel low molecular
RT   weight neuronal intermediate filament protein expressed in Xenopus
RT   laevis.";
RL   J. Neurosci. 12:3010-3024(1992).
CC   -!- FUNCTION: Neurofilaments usually contain three intermediate filament
CC       proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of
CC       neuronal caliber. May additionally cooperate with other neuronal
CC       intermediate filament proteins to form neuronal filamentous networks
CC       (By similarity). {ECO:0000250|UniProtKB:P08551}.
CC   -!- SUBUNIT: Forms homodimers (in vitro). {ECO:0000250|UniProtKB:P19527}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, axon
CC       {ECO:0000250|UniProtKB:P08551}. Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:P08551}.
CC   -!- MISCELLANEOUS: NF-L is the most abundant of the three neurofilament
CC       proteins and, as the other nonepithelial intermediate filament
CC       proteins, it can form homomeric 10-nm filaments.
CC   -!- SIMILARITY: Belongs to the intermediate filament family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR   EMBL; M86654; AAA83018.1; -; mRNA.
DR   PIR; B44841; B44841.
DR   RefSeq; NP_001081414.1; NM_001087945.1.
DR   AlphaFoldDB; P35616; -.
DR   SMR; P35616; -.
DR   PRIDE; P35616; -.
DR   GeneID; 397822; -.
DR   KEGG; xla:397822; -.
DR   CTD; 397822; -.
DR   Xenbase; XB-GENE-866125; nefl.L.
DR   OrthoDB; 655109at2759; -.
DR   Proteomes; UP000186698; Chromosome 3L.
DR   Bgee; 397822; Expressed in brain and 14 other tissues.
DR   GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR   GO; GO:0005883; C:neurofilament; IEA:InterPro.
DR   GO; GO:0061564; P:axon development; IEA:InterPro.
DR   InterPro; IPR018039; IF_conserved.
DR   InterPro; IPR039008; IF_rod_dom.
DR   InterPro; IPR006821; Intermed_filament_DNA-bd.
DR   InterPro; IPR027692; NF-L.
DR   PANTHER; PTHR45652:SF8; PTHR45652:SF8; 1.
DR   Pfam; PF00038; Filament; 1.
DR   Pfam; PF04732; Filament_head; 1.
DR   SMART; SM01391; Filament; 1.
DR   PROSITE; PS00226; IF_ROD_1; 1.
DR   PROSITE; PS51842; IF_ROD_2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cell projection; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Intermediate filament; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..544
FT                   /note="Neurofilament light polypeptide"
FT                   /id="PRO_0000063792"
FT   DOMAIN          84..394
FT                   /note="IF rod"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT   REGION          2..87
FT                   /note="Head"
FT   REGION          88..119
FT                   /note="Coil 1A"
FT   REGION          120..132
FT                   /note="Linker 1"
FT   REGION          133..228
FT                   /note="Coil 1B"
FT   REGION          229..246
FT                   /note="Linker 12"
FT   REGION          247..265
FT                   /note="Coil 2A"
FT   REGION          266..274
FT                   /note="Linker 2"
FT   REGION          275..390
FT                   /note="Coil 2B"
FT   REGION          391..544
FT                   /note="Tail"
FT   REGION          391..435
FT                   /note="Tail, subdomain A"
FT   REGION          436..544
FT                   /note="Tail, subdomain B (acidic)"
FT   REGION          450..544
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        461..531
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   544 AA;  61862 MW;  76D911B896E97201 CRC64;
     MSSYSYDPYY TPYKRRVVES SPRVHIRSSY VSPSRTTYSP LVSTTMRRSY ATSSSSSFLP
     SVDTMDLSQV AAISSDLKIV RTQEKVQLQD LNDRFANFIE RVHELEQRNK VLEAELLLLR
     QKHNEPSRLR DMYEKEVRDV RLAQEEASGD RQTLRNERER LEDALRVLQG RYEEEAMSRE
     DSEARLLDVR KEADMAALAR VELEKRMDSL LDEIAFLKKV HEEELSQLQS QVQYAQVSLE
     VEVAKPDLSS ALRDIRGQYE KLAAKNMQSA EEWFKSRFTV LTQSAARNTD AVRAAKDEMS
     ESRRMLSAKG LEIEACRGVN EALQRQIQEL EDKQSGEIAG MQDAINKLEE ELRNTKSEMA
     RYLKEYQDLL NVKMALDIEI AAYRKLLEGE ETRLSFSGVG AITSGYTQSA PVFGRSAYSL
     QSSSYMTSRA FPTYYSSHVQ EEQLDIEETI ESSRAEEAKA EAPEEEEEEA AEEEGEGGEE
     AEEEGEEGEE AKEEEAEEEG GQEKEEEGEE GEGEAEAEGD GEEEGESKGD EAAEEESEKK
     EKKK