NFRA_ECOLI
ID NFRA_ECOLI Reviewed; 990 AA.
AC P31600;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Bacteriophage adsorption protein A {ECO:0000305};
DE AltName: Full=Bacteriophage N4 adsorption protein A;
DE Flags: Precursor;
GN Name=nfrA; OrderedLocusNames=b0568, JW0557;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8226649; DOI=10.1128/jb.175.21.7081-7085.1993;
RA Kiino D.R., Singer M.S., Rothman-Denes L.B.;
RT "Two overlapping genes encoding membrane proteins required for
RT bacteriophage N4 adsorption.";
RL J. Bacteriol. 175:7081-7085(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP INTERACTION WITH N4 PHAGE NON-CONTRACTILE SHEATH PROTEIN (MICROBIAL
RP INFECTION), AND FUNCTION (MICROBIAL INFECTION).
RX PubMed=19011026; DOI=10.1128/jb.01423-08;
RA McPartland J., Rothman-Denes L.B.;
RT "The tail sheath of bacteriophage N4 interacts with the Escherichia coli
RT receptor.";
RL J. Bacteriol. 191:525-532(2009).
CC -!- FUNCTION: (Microbial infection) Allows N4 phage attachment by binding
CC to the viral non-contractile sheath protein.
CC {ECO:0000269|PubMed:19011026}.
CC -!- SUBUNIT: (Microbial infection) Interacts with N4 phage non-contractile
CC sheath protein; this interaction is essential for viral adsorption to
CC the host. {ECO:0000269|PubMed:19011026}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L16945; AAC36849.1; -; Unassigned_DNA.
DR EMBL; U82598; AAB40766.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73669.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35202.1; -; Genomic_DNA.
DR PIR; B49351; B49351.
DR RefSeq; NP_415100.1; NC_000913.3.
DR RefSeq; WP_000662357.1; NZ_LN832404.1.
DR AlphaFoldDB; P31600; -.
DR BioGRID; 4259890; 194.
DR BioGRID; 849145; 1.
DR DIP; DIP-10332N; -.
DR IntAct; P31600; 11.
DR STRING; 511145.b0568; -.
DR jPOST; P31600; -.
DR PaxDb; P31600; -.
DR PRIDE; P31600; -.
DR EnsemblBacteria; AAC73669; AAC73669; b0568.
DR EnsemblBacteria; BAA35202; BAA35202; BAA35202.
DR GeneID; 944741; -.
DR KEGG; ecj:JW0557; -.
DR KEGG; eco:b0568; -.
DR PATRIC; fig|1411691.4.peg.1706; -.
DR EchoBASE; EB1691; -.
DR eggNOG; COG4783; Bacteria.
DR HOGENOM; CLU_012342_0_0_6; -.
DR OMA; WDHALGE; -.
DR BioCyc; EcoCyc:EG11740-MON; -.
DR PRO; PR:P31600; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0046813; P:receptor-mediated virion attachment to host cell; IMP:EcoCyc.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR025137; NfrA_C.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13283; NfrA_C; 1.
DR SMART; SM00028; TPR; 2.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 3.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Membrane; Reference proteome; Repeat; Signal;
KW TPR repeat.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..990
FT /note="Bacteriophage adsorption protein A"
FT /id="PRO_0000021809"
FT REPEAT 81..114
FT /note="TPR 1"
FT REPEAT 612..645
FT /note="TPR 2"
FT REPEAT 646..679
FT /note="TPR 3"
SQ SEQUENCE 990 AA; 111308 MW; AAA616665D1C2E72 CRC64;
MKENNLNRVI GWSGLLLTSL LSTSALADNI GTSAEELGLS DYRHFVIYPR LDKALKAQKN
NDEATAIREF EYIHQQVPDN IPLTLYLAEA YRHFGHDDRA RLLLEDQLKR HPGDARLERS
LAAIPVEVKS VTTVEELLAQ QKACDAAPTL RCRSEVGQNA LRLAQLPVAR AQLNDATFAA
SPEGKTLRTD LLQRAIYLKQ WSQADTLYNE ARQQNTLSAA ERRQWFDVLL AGQLDDRILA
LQSQGIFTDP QSYITYATAL AYRGEKARLQ HYLIENKPLF TTDAQEKSWL YLLSKYSANP
VQALANYTVQ FADNRQYVVG ATLPVLLKEG QYDAAQKLLA TLPANEMLEE RYAVSVATRN
KAEALRLARL LYQQEPANLT RLDQLTWQLM QNEQSREAAD LLLQRYPFQG DARVSQTLMA
RLASLLESHP YLATPAKVAI LSKPLPLAEQ RQWQSQLPGI ADNCPAIVRL LGDMSPSYDA
AAWNRLAKCY RDTLPGVALY AWLQAEQRQP SAWQHRAVAY QAYQVEDYAT ALAAWQKISL
HDMSNEDLLA AANTAQAAGN GAARDRWLQQ AEKRGLGSNA LYWWLHAQRY IPGQPELALN
DLTRSINIAP SANAYVARAT IYRQRHNVPA AVSDLRAALE LEPNNSNTQA ALGYALWDSG
DIAQSREMLE PAHKGLPDDP ALIRQLAYVN QRLDDMPATQ HYARLVIDDI DNQALITPLT
PEQNQQRFNF RRLHEEVGRR WTFSFDSSIG LRSGAMSTAN NNVGGAAPGK SYRSYGQLEA
EYRIGRNMLL EGDLLSVYSR VFADTGENGV MMPVKNPMSG TGLRWKPLRD QIFFIAVEQQ
LPLNGQNGAS DTMLRASASF FNGGKYSDEW HPNGSGWFAQ NLYLDAAQYI RQDIQAWTAD
YRVSWHQKVA NGQTIEPYAH VQDNGYRDKG TQGAQLGGVG VRWNIWTGET HYDAWPHKVS
LGVEYQHTFK AINQRNGERN NAFLTIGVHW
