ID   NFRB_ECOLI              Reviewed;         745 AA.
AC   P0AFA5; P31599;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Bacteriophage adsorption protein B {ECO:0000305};
DE   AltName: Full=Bacteriophage N4 adsorption protein B;
GN   Name=nfrB; OrderedLocusNames=b0569, JW0558;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8226649; DOI=10.1128/jb.175.21.7081-7085.1993;
RA   Kiino D.R., Singer M.S., Rothman-Denes L.B.;
RT   "Two overlapping genes encoding membrane proteins required for
RT   bacteriophage N4 adsorption.";
RL   J. Bacteriol. 175:7081-7085(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Required for bacteriophage N4 adsorption. May be a component
CC       of the phage receptor.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:15919996}.
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DR   EMBL; L16945; AAC36848.1; -; Unassigned_DNA.
DR   EMBL; U82598; AAB40767.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73670.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35203.1; -; Genomic_DNA.
DR   PIR; A49351; A49351.
DR   RefSeq; NP_415101.1; NC_000913.3.
DR   RefSeq; WP_000383932.1; NZ_LN832404.1.
DR   AlphaFoldDB; P0AFA5; -.
DR   SMR; P0AFA5; -.
DR   BioGRID; 4262937; 95.
DR   DIP; DIP-10333N; -.
DR   IntAct; P0AFA5; 2.
DR   STRING; 511145.b0569; -.
DR   PaxDb; P0AFA5; -.
DR   PRIDE; P0AFA5; -.
DR   EnsemblBacteria; AAC73670; AAC73670; b0569.
DR   EnsemblBacteria; BAA35203; BAA35203; BAA35203.
DR   GeneID; 945849; -.
DR   KEGG; ecj:JW0558; -.
DR   KEGG; eco:b0569; -.
DR   PATRIC; fig|1411691.4.peg.1705; -.
DR   EchoBASE; EB1690; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_019733_1_0_6; -.
DR   OMA; KTMHDFP; -.
DR   PhylomeDB; P0AFA5; -.
DR   BioCyc; EcoCyc:EG11739-MON; -.
DR   PRO; PR:P0AFA5; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0015627; C:type II protein secretion system complex; IBA:GO_Central.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IBA:GO_Central.
DR   Gene3D; 3.30.300.160; -; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR037257; T2SS_E_N_sf.
DR   InterPro; IPR007831; T2SS_GspE_N.
DR   Pfam; PF13632; Glyco_trans_2_3; 1.
DR   Pfam; PF05157; T2SSE_N; 1.
DR   SUPFAM; SSF160246; SSF160246; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   4: Predicted;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..745
FT                   /note="Bacteriophage adsorption protein B"
FT                   /id="PRO_0000096796"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   745 AA;  85319 MW;  B596A5D5B64654C0 CRC64;
     MDWLLDVFAT WLYGLKVIAI TLAVIMFISG LDDFFIDVVY WVRRIKRKLS VYRRYPRMSY
     RELYKPDEKP LAIMVPAWNE TGVIGNMAEL AATTLDYENY HIFVGTYPND PDTQRDVDEV
     CARFPNVHKV VCARPGPTSK ADCLNNVLDA ITQFERSANF AFAGFILHDA EDVISPMELR
     LFNYLVERKD LIQIPVYPFE REWTHFTSMT YIDEFSELHG KDVPVREALA GQVPSAGVGT
     CFSRRAVTAL LADGDGIAFD VQSLTEDYDI GFRLKEKGMT EIFVRFPVVD EAKEREQRKF
     LQHARTSNMI CVREYFPDTF STAVRQKSRW IIGIVFQGFK THKWTSSLTL NYFLWRDRKG
     AISNFVSFLA MLVMIQLLLL LAYESLWPDA WHFLSIFSGS AWLMTLLWLN FGLMVNRIVQ
     RVIFVTGYYG LTQGLLSVLR LFWGNLINFM ANWRALKQVL QHGDPRRVAW DKTTHDFPSV
     TGDTRSLRPL GQILLENQVI TEEQLDTALR NRVEGLRLGG SMLMQGLISA EQLAQALAEQ
     NGVAWESIDA WQIPSSLIAE MPASVALHYA VLPLRLENDE LIVGSEDGID PVSLAALTRK
     VGRKVRYVIV LRGQIVTGLR HWYARRRGHD PRAMLYNAVQ HQWLTEQQAG EIWRQYVPHQ
     FLFAEILTTL GHINRSAINV LLLRHERSSL PLGKFLVTEG VISQETLDRV LTIQRELQVS
     MQSLLLKAGL NTEQVAQLES ENEGE