NFT1_YEAST
ID NFT1_YEAST Reviewed; 1218 AA.
AC P0CE68; D6VXG4; P36028; P36171; Q53ZY4; Q6Q5L3;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=ABC transporter NFT1;
DE AltName: Full=New full-length MRP-type transporter 1;
GN Name=NFT1; OrderedLocusNames=YKR103W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCC family.
CC Conjugate transporter (TC 3.A.1.208) subfamily. {ECO:0000305}.
CC -!- CAUTION: This is a truncated version of the ABC transporter NFT1. S288c
CC has a stop codon in position 1219, which disrupts the gene coding for
CC this protein and produces two ORFs YKR103W and YKR104W. A contiguous
CC sequence for ABC transporter NFT1 can be found in strains Sigma 1278B,
CC EG123 and SK1 (AC P0CE70). {ECO:0000305}.
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DR EMBL; Z28328; CAA82183.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09254.1; -; Genomic_DNA.
DR PIR; S38182; S38182.
DR RefSeq; NP_013029.3; NM_001179893.3.
DR AlphaFoldDB; P0CE68; -.
DR SMR; P0CE68; -.
DR BioGRID; 34234; 81.
DR IntAct; P0CE68; 23.
DR MINT; P0CE68; -.
DR STRING; 4932.YKR103W; -.
DR iPTMnet; P0CE68; -.
DR PaxDb; P0CE68; -.
DR PRIDE; P0CE68; -.
DR EnsemblFungi; YKR103W_mRNA; YKR103W; YKR103W.
DR GeneID; 853978; -.
DR KEGG; sce:YKR103W; -.
DR SGD; S000001811; NFT1.
DR VEuPathDB; FungiDB:YKR103W; -.
DR eggNOG; KOG0054; Eukaryota.
DR GeneTree; ENSGT00940000176323; -.
DR HOGENOM; CLU_000604_27_6_1; -.
DR InParanoid; P0CE68; -.
DR OMA; AILANFE; -.
DR BioCyc; YEAST:G3O-32065-MON; -.
DR Reactome; R-SCE-382556; ABC-family proteins mediated transport.
DR Reactome; R-SCE-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-SCE-9646399; Aggrephagy.
DR PRO; PR:P0CE68; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P0CE68; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0016236; P:macroautophagy; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1560.10; -; 2.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00664; ABC_membrane; 2.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF90123; SSF90123; 2.
DR PROSITE; PS50929; ABC_TM1F; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Glycoprotein; Membrane; Nucleotide-binding;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1218
FT /note="ABC transporter NFT1"
FT /id="PRO_0000093462"
FT TOPO_DOM 1..29
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 51..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 125..130
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 152..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 191..201
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 223..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 324..351
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..374
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 375..449
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 471..481
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..504
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 505..558
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 580..584
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 585..605
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 606..953
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 954..974
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 975..1013
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1014..1034
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 1035..1082
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1083..1105
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 1106..1109
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1110..1132
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 1133..1197
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1198..1218
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 311..621
FT /note="ABC transmembrane type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 651..892
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 961..1218
FT /note="ABC transmembrane type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT REGION 410..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 892..926
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..434
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 686..693
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1218 AA; 137997 MW; 804810FD5BF0C210 CRC64;
MIKNGTCPYW ERDDLSECAR REYIEFKFPL FILLTGMIYA FCKVFRAFYL RGKNHTNEAP
EFEEQGNGNH EYARFSVLRL KSAWESRSFC NVNNRSTFDK FKKFIEGAFI VLQLTIHLYI
LSSMPMDNKK FFHQGFLVQM FLWILLLVVI TLRLISASQS FRWVLACKRD LWAVSFYSYA
SLFTLSILPL RSVFIGKIKD KIMVKYIISE TFIDLALLLL LSTSSIEGTR YSFLVENENK
KLPPAPTVFG LLTFSRIDRL IWKAYKHCLG NADIWDLDIN NKSIAILANF EMSSKKGRLL
PNIICYFKAV FISQLFLAFV SSFLNFVPSL LMPRILSYVN DPKSKSWNLV SLYVSSMLVS
KIIATTCRGQ GLFLGEKGTM QLRTVLISNI YSKTLRRTIL KDSTTSLQKN ASTSFEENPD
SSEAEPRKKS SRKDNSVNNV MSIDAFKVSE AMNTFYLACE AVFMTVTALM ILYSLLGWSA
FAGTFALLAM IPLNFWCATF YGNYQADQLI LTDKRTSGIS EALNSIRVIK LLAWENLFYQ
KIINVRDGEI RLLKKKATIF FLNHLIWFFG PTLVSAITFS VFIKFQNQTL TPTIAFTALS
LFAILRTPMD QIASTVSLLI QSFISLERIQ DYLNESETRK YEILEQSNTK FGFEDASMEW
EAAETSFKLK NISIDFKLNS LNAIIGPTGS GKSSLLLGLL GELNLLSGKI YVPTVESRDD
LEIGKDGMTN SMAYCSQTPW LISGTIKDNV VFGEIFNKQK FDDVMKSCCL DKDIKAMTAG
IRTDVGDGGF SLSGGQQQRI ALARAIYSSS RYLILDDCLS AVDPETALYI YEECLCGPMM
KGRTCIITSH NISLVTKRAD WLVILDRGEV KSQGKPSDLI KSNEFLRESI NNDSKNTTHN
QIDLKRSTTS KKTKNGDPEG GNSQDEVCAE VENFEETKME GSVKFSAYKW LADYFGGLGV
VFVFTSSSIL IHGITLSQGF WLRYWLDTGS SGSKSTWLYR IVEGHSNIYF LLTYIIIGLV
SSFLTSGKVW IAIISGTNVT KKIFAKLLSS ILYAKLRFHN VTPTGRIMNR FSKDMDIIDQ
QLIPNFEGLS YSVVVCLWII LLIGYVTPQF LLFAIPLCAL YYTVCTLYLR ASRELKRIDN
INISPIHQLF AEAIKGVTTI RALADERRFI TQSLVAIDRS NAPFFYLNMA TEWITYRVDI
IGTLVLFSSS VMIIMKAS
