NFU1_DROME
ID NFU1_DROME Reviewed; 283 AA.
AC Q8SY96; Q9I806; Q9U4F4;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=NFU1 iron-sulfur cluster scaffold homolog, mitochondrial {ECO:0000250|UniProtKB:Q9UMS0};
DE Flags: Precursor;
GN ORFNames=CG32500 {ECO:0000312|FlyBase:FBgn0285970};
GN and
GN ORFNames=CG32857 {ECO:0000312|FlyBase:FBgn0052857};
GN and
GN ORFNames=CG33502 {ECO:0000312|FlyBase:FBgn0053502};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF16622.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAF16622.1}, and
RC Canton-S {ECO:0000312|EMBL:AAF28338.1};
RA Mukhopadhyay M., DeSousa D., Pelka P., Robert V., Pelisson A., Bucheton A.,
RA Campos A.R.;
RT "Cloning and characterization of the Drosophila dip1 (disco interacting
RT protein) gene, a new member of the Staufen family of RNA binding
RT proteins.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:AAG22462.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (CG32500/CG32857/CG33502).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAG22462.2};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAG22462.2}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAL49319.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL49319.1};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of
CC mitochondrial iron-sulfur proteins. {ECO:0000250|UniProtKB:Q9UMS0}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF16622.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAF28338.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF182444; AAF16622.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF213379; AAF28338.1; ALT_INIT; mRNA.
DR EMBL; AE014298; AAG22462.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09001.1; -; Genomic_DNA.
DR EMBL; AE014298; AAN09005.2; -; Genomic_DNA.
DR EMBL; AY071697; AAL49319.1; -; mRNA.
DR RefSeq; NP_728443.1; NM_167766.4.
DR RefSeq; NP_728447.2; NM_167770.3.
DR RefSeq; NP_996529.1; NM_206806.3.
DR AlphaFoldDB; Q8SY96; -.
DR SMR; Q8SY96; -.
DR BioGRID; 76847; 1.
DR BioGRID; 77725; 1.
DR BioGRID; 77728; 1.
DR STRING; 7227.FBpp0070051; -.
DR PaxDb; Q8SY96; -.
DR PRIDE; Q8SY96; -.
DR DNASU; 2768879; -.
DR EnsemblMetazoa; FBtr0070052; FBpp0070051; FBgn0052857.
DR EnsemblMetazoa; FBtr0070055; FBpp0070054; FBgn0053502.
DR EnsemblMetazoa; FBtr0077374; FBpp0077066; FBgn0285970.
DR GeneID; 2768875; -.
DR GeneID; 2768879; -.
DR GeneID; 318252; -.
DR KEGG; dme:Dmel_CG32500; -.
DR KEGG; dme:Dmel_CG32857; -.
DR KEGG; dme:Dmel_CG33502; -.
DR UCSC; CG32500-RA; d. melanogaster.
DR FlyBase; FBgn0285970; CG32500.
DR FlyBase; FBgn0052857; CG32857.
DR FlyBase; FBgn0053502; CG33502.
DR VEuPathDB; VectorBase:FBgn0052857; -.
DR VEuPathDB; VectorBase:FBgn0053502; -.
DR VEuPathDB; VectorBase:FBgn0285970; -.
DR eggNOG; KOG2358; Eukaryota.
DR GeneTree; ENSGT00390000011296; -.
DR HOGENOM; CLU_060555_0_2_1; -.
DR InParanoid; Q8SY96; -.
DR OMA; MHWISEV; -.
DR OrthoDB; 1016782at2759; -.
DR PhylomeDB; Q8SY96; -.
DR PRO; PR:Q8SY96; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0052857; Expressed in gastric caecum (Drosophila) and 24 other tissues.
DR Genevisible; Q8SY96; DM.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IBA:GO_Central.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; IBA:GO_Central.
DR Gene3D; 3.30.1370.70; -; 1.
DR Gene3D; 3.30.300.130; -; 1.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR014824; Nfu/NifU_N.
DR InterPro; IPR036498; Nfu/NifU_N_sf.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR Pfam; PF08712; Nfu_N; 1.
DR Pfam; PF01106; NifU; 1.
DR SMART; SM00932; Nfu_N; 1.
DR SUPFAM; SSF110836; SSF110836; 1.
DR SUPFAM; SSF117916; SSF117916; 1.
PE 2: Evidence at transcript level;
KW Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..65
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 66..283
FT /note="NFU1 iron-sulfur cluster scaffold homolog,
FT mitochondrial"
FT /evidence="ECO:0000255"
FT /id="PRO_0000388698"
FT REGION 182..250
FT /note="NifU"
FT /evidence="ECO:0000255"
FT BINDING 219
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250|UniProtKB:Q9UMS0"
FT BINDING 222
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250|UniProtKB:Q9UMS0"
SQ SEQUENCE 283 AA; 31296 MW; E7E7016A018E28F1 CRC64;
MSKFLSQAAL NTLRNTRLGS RQLVRSFKGI SNTRNHRIPA HQESGCGHSV GCGLLELRMP
VACRRSMFIQ TQDTPNPESL KFLPGVDVLG KGNTYDFPNG TTAHNSPLAK LLFRVEGVKG
VFFGADFVTI SKQEGAEWSL IKPEVFAVIM DFFASGLPVL NDAQPNADTE ILEDDDETVM
MIKELLDTRI RPTVQEDGGD IVFMGYEGGV VKLKMQGSCS SCPSSIVTLK NGVQNMLQFY
IPEVESVEQV FDEADRMIES EFERFEKNLK TLKQQEPSGG GPH
