ID   NFU1_DROPS              Reviewed;         286 AA.
AC   B5DKJ8;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=NFU1 iron-sulfur cluster scaffold homolog, mitochondrial {ECO:0000250|UniProtKB:Q9UMS0};
DE   Flags: Precursor;
GN   ORFNames=GA22888;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1] {ECO:0000312|EMBL:EDY72048.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of
CC       mitochondrial iron-sulfur proteins. {ECO:0000250|UniProtKB:Q9UMS0}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; CH379063; EDY72048.1; -; Genomic_DNA.
DR   RefSeq; XP_002133420.1; XM_002133384.2.
DR   AlphaFoldDB; B5DKJ8; -.
DR   SMR; B5DKJ8; -.
DR   STRING; 7237.FBpp0273234; -.
DR   EnsemblMetazoa; FBtr0274796; FBpp0273234; FBgn0244290.
DR   GeneID; 6901885; -.
DR   KEGG; dpo:Dpse_GA22888; -.
DR   eggNOG; KOG2358; Eukaryota.
DR   HOGENOM; CLU_060555_0_2_1; -.
DR   InParanoid; B5DKJ8; -.
DR   OMA; AIMEHYM; -.
DR   Proteomes; UP000001819; Chromosome X.
DR   Bgee; FBgn0244290; Expressed in insect adult head and 2 other tissues.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   Gene3D; 3.30.1370.70; -; 1.
DR   Gene3D; 3.30.300.130; -; 1.
DR   InterPro; IPR034904; FSCA_dom_sf.
DR   InterPro; IPR014824; Nfu/NifU_N.
DR   InterPro; IPR036498; Nfu/NifU_N_sf.
DR   InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR   Pfam; PF08712; Nfu_N; 1.
DR   Pfam; PF01106; NifU; 1.
DR   SMART; SM00932; Nfu_N; 1.
DR   SUPFAM; SSF110836; SSF110836; 1.
DR   SUPFAM; SSF117916; SSF117916; 1.
PE   3: Inferred from homology;
KW   Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..15
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..286
FT                   /note="NFU1 iron-sulfur cluster scaffold homolog,
FT                   mitochondrial"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000388700"
FT   REGION          182..250
FT                   /note="NifU"
FT                   /evidence="ECO:0000255"
FT   REGION          267..286
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         219
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UMS0"
FT   BINDING         222
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UMS0"
SQ   SEQUENCE   286 AA;  31516 MW;  889A1C324D6676F8 CRC64;
     MSKLLSYTAR IILRNSRTTV RQLGQEGVRG FAGFVSGQRN APQPAYGRPV PGLLRQKMVS
     SIGKRSMFIQ TQDTPNPDSL KFLPGVEVLG KGNTYDFPSG TAAHCSPLAK LLFRVEGVRA
     VFFGSDFITI SKEESAEWSL IKPEVFAVIM DFFASGLPIL HEARPNADTE ILDDDDETVM
     MIKELLDTRI RPTVQEDGGD IVFISYENGV VKLKMQGSCS SCPSSIVTLK NGVQNMLQFY
     IPEVESVEQV FDDVDRMADK EFERFEKNLK QKEPAGAPVG IGGGPN