ID   NFU1_DROWI              Reviewed;         289 AA.
AC   B4NE93;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=NFU1 iron-sulfur cluster scaffold homolog, mitochondrial {ECO:0000250|UniProtKB:Q9UMS0};
DE   Flags: Precursor;
GN   ORFNames=GK25604;
OS   Drosophila willistoni (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7260;
RN   [1] {ECO:0000312|EMBL:EDW82062.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14030-0811.24 {ECO:0000312|EMBL:EDW82062.1};
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of
CC       mitochondrial iron-sulfur proteins. {ECO:0000250|UniProtKB:Q9UMS0}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the NifU family. {ECO:0000305}.
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DR   EMBL; CH964239; EDW82062.1; -; Genomic_DNA.
DR   RefSeq; XP_002071076.2; XM_002071040.2.
DR   AlphaFoldDB; B4NE93; -.
DR   SMR; B4NE93; -.
DR   STRING; 7260.FBpp0254747; -.
DR   EnsemblMetazoa; FBtr0256255; FBpp0254747; FBgn0227563.
DR   GeneID; 6648753; -.
DR   KEGG; dwi:6648753; -.
DR   eggNOG; KOG2358; Eukaryota.
DR   HOGENOM; CLU_060555_0_2_1; -.
DR   InParanoid; B4NE93; -.
DR   OMA; AIMEHYM; -.
DR   OrthoDB; 1016782at2759; -.
DR   PhylomeDB; B4NE93; -.
DR   Proteomes; UP000007798; Unassembled WGS sequence.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   Gene3D; 3.30.1370.70; -; 1.
DR   Gene3D; 3.30.300.130; -; 1.
DR   InterPro; IPR034904; FSCA_dom_sf.
DR   InterPro; IPR014824; Nfu/NifU_N.
DR   InterPro; IPR036498; Nfu/NifU_N_sf.
DR   InterPro; IPR035433; NFU1-like.
DR   InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR   Pfam; PF08712; Nfu_N; 1.
DR   Pfam; PF01106; NifU; 1.
DR   PIRSF; PIRSF036773; HIRIP5; 1.
DR   SMART; SM00932; Nfu_N; 1.
DR   SUPFAM; SSF110836; SSF110836; 1.
DR   SUPFAM; SSF117916; SSF117916; 1.
PE   3: Inferred from homology;
KW   Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..56
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           57..289
FT                   /note="NFU1 iron-sulfur cluster scaffold homolog,
FT                   mitochondrial"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000388704"
FT   REGION          183..251
FT                   /note="NifU"
FT                   /evidence="ECO:0000255"
FT   REGION          267..289
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         220
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UMS0"
FT   BINDING         223
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UMS0"
SQ   SEQUENCE   289 AA;  31729 MW;  D9CC7CA90046EAFA CRC64;
     MAKLISYAKG GFLRNTRLTS RAVPQVYQHA TSSRGFVHLT SSVAQSSAIH VSTPSTPSLM
     LIMGKRTMFI QTQDTPNPES LKFLPGVEVL GKGNTYDFPS VAAAHCSPLA KLLFRVEGVR
     SVFFGSDFIT ISKEEAAEWG LIKPEVFAVI MDFFASGLPI LHEARNNADT EILEDDDETV
     MMIKELLDTR IRPTVQEDGG DIVFMSYDNG VVKLKMQGSC SSCPSSIVTL KNGVQNMLQF
     YIPEVESVEQ VFDEADKMAD KEFERFERNL KQKDTSSTAP VGIGGGPAN