NFYA1_CAEEL
ID NFYA1_CAEEL Reviewed; 482 AA.
AC G5EEG1;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Nuclear transcription factor Y subunit nfya-1 {ECO:0000305};
DE AltName: Full=CAAT box DNA-binding protein subunit nfya-1 {ECO:0000305};
GN Name=nfya-1 {ECO:0000312|WormBase:T08D10.1};
GN ORFNames=T08D10.1 {ECO:0000312|WormBase:T08D10.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15704008; DOI=10.1007/s10735-004-6017-6;
RA Franchini A., Imbriano C., Peruzzi E., Mantovani R., Ottaviani E.;
RT "Expression of the CCAAT-binding factor NF-Y in Caenorhabditis elegans.";
RL J. Mol. Histol. 36:139-145(2005).
RN [3] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN NF-Y COMPLEX, INTERACTION WITH NFYB-1; NFYC-1
RP AND MES-3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 102-GLN--LEU-482 AND GLN-107.
RX PubMed=17574230; DOI=10.1016/j.ydbio.2007.05.021;
RA Deng H., Sun Y., Zhang Y., Luo X., Hou W., Yan L., Chen Y., Tian E.,
RA Han J., Zhang H.;
RT "Transcription factor NFY globally represses the expression of the C.
RT elegans Hox gene Abdominal-B homolog egl-5.";
RL Dev. Biol. 308:583-592(2007).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23933492; DOI=10.1016/j.ydbio.2013.08.001;
RA Milton A.C., Packard A.V., Clary L., Okkema P.G.;
RT "The NF-Y complex negatively regulates Caenorhabditis elegans tbx-2
RT expression.";
RL Dev. Biol. 382:38-47(2013).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=25873636; DOI=10.1534/g3.115.018101;
RA Milton A.C., Okkema P.G.;
RT "Caenorhabditis elegans TBX-2 Directly Regulates Its Own Expression in a
RT Negative Autoregulatory Loop.";
RL G3 (Bethesda) 5:1177-1186(2015).
CC -!- FUNCTION: Component of the sequence-specific heterotrimeric
CC transcription factor (nfya-1-NF-Y) which specifically recognizes a 5'-
CC CCAAT-3' box motif found in the promoters of its target genes to
CC regulate their expression and control cellular identity in particular
CC tissue types (PubMed:17574230). In association with the components in
CC the nfya-1-NF-Y complex, represses the expression of the T-box
CC transcription factor tbx-2 throughout larval development, which most
CC likely restricts its expression to certain tissues (PubMed:23933492).
CC May act to repress txb-2 expression in conjunction with tbx-2 itself,
CC which has an autoregulatory role (Probable). With the components in
CC this complex, negatively regulates the expression of the homeobox
CC protein egl-5 to spatially restrict its expression in tissues such as
CC the head (PubMed:17574230). May regulate egl-5 expression in
CC association with the mes-2-mes-3-mes-6 complex (PubMed:17574230).
CC {ECO:0000269|PubMed:17574230, ECO:0000269|PubMed:23933492,
CC ECO:0000305|PubMed:25873636}.
CC -!- SUBUNIT: Forms a heterotrimeric transcription factor complex (nfya-1-
CC NF-Y complex) composed of nfya-1, nfyb-1 and nfyc-1, which binds to 5'-
CC CCAAT-3' box motif in the promoters of its target genes
CC (PubMed:17574230). Interacts with the nfyb-1 and nfyc-1 dimer; the
CC interaction is required for subsequent binding to the 5'-CCAAT-3' box
CC motif in DNA (PubMed:17574230). Does not interact with either nfyb-1 or
CC nfyc-1 in their monomeric form (PubMed:17574230). Interacts with mes-3
CC (PubMed:17574230). {ECO:0000269|PubMed:17574230}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17574230}.
CC -!- TISSUE SPECIFICITY: Expressed in certain parts of the gonads with high
CC expression in fertilized oocytes in the uterus and mature oocytes from
CC the distal to the proximal arm of the gonad, but weak expression in the
CC syncytial ovaries and immature oocytes at the beginning of the proximal
CC arm of the gonad (PubMed:15704008). Highly expressed in the head
CC ganglia neurons and the developing hermaphrodite vulva and male tail
CC (PubMed:17574230). Weakly expressed in most somatic cells
CC (PubMed:17574230). Not expressed in the intestine, the hypodermis, body
CC wall muscle surrounding the pseudocoelomic space, secretory cells in
CC the pharyngeal terminal bulb wall, in the small ganglia surrounding the
CC pharynx and in the neurons running anteriorly to the sensory organs in
CC the head (PubMed:15704008). {ECO:0000269|PubMed:15704008,
CC ECO:0000269|PubMed:17574230}.
CC -!- DEVELOPMENTAL STAGE: Expressed at all developmental stages
CC (PubMed:15704008, PubMed:17574230). In larvae, highly expressed in the
CC head ganglia neurons and the developing hermaphrodite vulva and male
CC tail, while expression in most somatic cells is weak (PubMed:17574230).
CC Not expressed in the gonads in larval stages (PubMed:15704008).
CC {ECO:0000269|PubMed:15704008, ECO:0000269|PubMed:17574230}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in ectopic
CC expression of the homeobox protein egl-5 in the head region of
CC hermaphrodites (PubMed:17574230). RNAi-mediated knockdown results in
CC morphological defects in the male tail due to cell transformation
CC defects whereby anterior seam cells adopt a posterior seam cell fate,
CC which is indicated by ectopic expression of the ray-specific protein
CC pkd-2 in the anterior mid-body region (PubMed:17574230). This leads to
CC impaired cell identities of the rays derived from seam cells
CC (PubMed:17574230). These animals also have defective cell
CC transformations in the ventral cord and generate two extra serotonergic
CC CP neurons (PubMed:17574230). RNAi-mediated knockdown results in
CC ectopic expression of tbx-2 in the gut and seam cells of L4 stage
CC larvae and adults (PubMed:23933492). {ECO:0000269|PubMed:17574230,
CC ECO:0000269|PubMed:23933492}.
CC -!- SIMILARITY: Belongs to the NFYA/HAP2 subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00966}.
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DR EMBL; BX284606; CAA90639.1; -; Genomic_DNA.
DR PIR; T22754; T22754.
DR RefSeq; NP_509999.1; NM_077598.3.
DR AlphaFoldDB; G5EEG1; -.
DR ComplexPortal; CPX-5666; CCAAT-binding factor complex, nfya-1 variant.
DR IntAct; G5EEG1; 3.
DR MINT; G5EEG1; -.
DR STRING; 6239.T08D10.1; -.
DR EPD; G5EEG1; -.
DR PaxDb; G5EEG1; -.
DR PeptideAtlas; G5EEG1; -.
DR EnsemblMetazoa; T08D10.1.1; T08D10.1.1; WBGene00011614.
DR GeneID; 181368; -.
DR KEGG; cel:CELE_T08D10.1; -.
DR CTD; 181368; -.
DR WormBase; T08D10.1; CE03642; WBGene00011614; nfya-1.
DR eggNOG; KOG1561; Eukaryota.
DR GeneTree; ENSGT00390000015714; -.
DR HOGENOM; CLU_483342_0_0_1; -.
DR InParanoid; G5EEG1; -.
DR OMA; NCKLFQY; -.
DR OrthoDB; 1269806at2759; -.
DR SignaLink; G5EEG1; -.
DR PRO; PR:G5EEG1; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00011614; Expressed in embryo and 4 other tissues.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IPI:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IMP:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0110039; P:positive regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009888; P:tissue development; IMP:UniProtKB.
DR InterPro; IPR001289; NFYA.
DR PANTHER; PTHR12632; PTHR12632; 1.
DR Pfam; PF02045; CBFB_NFYA; 1.
DR PRINTS; PR00616; CCAATSUBUNTB.
DR SMART; SM00521; CBF; 1.
DR PROSITE; PS51152; NFYA_HAP2_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..482
FT /note="Nuclear transcription factor Y subunit nfya-1"
FT /id="PRO_0000450332"
FT DNA_BIND 336..361
FT /note="NFYA/HAP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT REGION 1..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 306..329
FT /note="Subunit association domain (SAD)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT COMPBIAS 49..63
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..160
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..376
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 102..482
FT /note="Missing: In bp4; ectopic expression of the homeobox
FT protein egl-5 in the head and mid-body regions."
FT /evidence="ECO:0000269|PubMed:17574230"
FT MUTAGEN 107
FT /note="Q->K: In bp5; ectopic expression of the homeobox
FT protein egl-5 in the head and mid-body regions."
FT /evidence="ECO:0000269|PubMed:17574230"
SQ SEQUENCE 482 AA; 53591 MW; 8D582B6B8EC95C2D CRC64;
MNGASRGDVQ RPTPVQAPAK RPIPPTVFRF EKASKIGTSL DTTPKELPIR RPVPLPGPRF
VKDVNPSPPA TSSPNVQTQC HQPPVVRSQT HQASVSQTTP TQTTPSQYTP ASVSTARAPQ
FHRSSHVTPS QQQRIEQAFG PAPVSQSQPQ NGSYIQYNEP STSVASTTTS FYSPNESEPV
FTHAISFKPN EPENGQKARE QLELLDSGKI NEINQSKVPT TIELPPNCKL FQYSWVLDGI
PRTLLVPMPM DATEEDVKAM LPKTLEMDSN LFNNARPRDE LPVLSFPNGT VPNVEMIGPK
VQQPMLVNPK QFNRIMRRRE MRQQLEASGR LPLARQKYLH ESRHLHALKR KRGLDGRFDN
TKTAESSSMV SSTTSPRKLK RRNRLPGEET SDSSSIVLPS PVEIQPKGGI VNSSMPSTST
GYINNGMDHQ TDYIEHQQPM STYDQYHDHV QYIAPDGNVN YHNNHDNGVD LTGSDGQSFT
NL
