NFYA2_ARATH
ID NFYA2_ARATH Reviewed; 295 AA.
AC Q9M9X4; O23631;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Nuclear transcription factor Y subunit A-2 {ECO:0000303|PubMed:11250072, ECO:0000303|PubMed:11867211};
DE Short=AtNF-YA-2 {ECO:0000303|PubMed:11250072, ECO:0000303|PubMed:11867211};
DE Short=AtNF-YA2 {ECO:0000303|PubMed:25490919};
DE AltName: Full=Transcriptional activator HAP2B;
GN Name=NFYA2 {ECO:0000303|PubMed:11250072, ECO:0000303|PubMed:11867211};
GN Synonyms=HAP2B;
GN OrderedLocusNames=At3g05690 {ECO:0000312|Araport:AT3G05690};
GN ORFNames=F18C1.4 {ECO:0000312|EMBL:AAF26128.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=9662544; DOI=10.1104/pp.117.3.1015;
RA Edwards D., Murray J.A.H., Smith A.G.;
RT "Multiple genes encoding the conserved CCAAT-box transcription factor
RT complex are expressed in Arabidopsis.";
RL Plant Physiol. 117:1015-1022(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11250072; DOI=10.1016/s0378-1119(01)00323-7;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana.";
RL Gene 264:173-185(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
RN [6]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=25490919; DOI=10.1105/tpc.114.132928;
RA Sato H., Mizoi J., Tanaka H., Maruyama K., Qin F., Osakabe Y., Morimoto K.,
RA Ohori T., Kusakabe K., Nagata M., Shinozaki K., Yamaguchi-Shinozaki K.;
RT "Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat
RT stress-induced gene expression by forming a heat stress-specific
RT transcriptional complex with NF-Y subunits.";
RL Plant Cell 26:4954-4973(2014).
RN [7]
RP REVIEW.
RX PubMed=28119722; DOI=10.3389/fpls.2016.02045;
RA Zhao H., Wu D., Kong F., Lin K., Zhang H., Li G.;
RT "The Arabidopsis thaliana Nuclear Factor Y Transcription Factors.";
RL Front. Plant Sci. 7:2045-2045(2016).
CC -!- FUNCTION: Stimulates the transcription of various genes by recognizing
CC and binding to a CCAAT motif in promoters (By similarity). Promotes the
CC expression of heat stress-inducible genes by contributing to the
CC formation of a heat stress-specific transcriptional complex with NF-Y
CC subunits (e.g. DPB3-1, NF-YA2 and NF-YB3) and DREB2A at the promoter of
CC target genes, thus promoting heat tolerance (PubMed:25490919).
CC {ECO:0000250|UniProtKB:Q9SFD8, ECO:0000269|PubMed:25490919}.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC (By similarity). NF-YB and NF-YC
CC must interact and dimerize for NF-YA association and DNA binding (By
CC similarity). Component of a heat stress-inducible transcriptional
CC complex with NF-YA and NF-YB subunits made, at least, of NFYA2, NFYB3
CC and DPB3-1 in cooperation with DREB2A (PubMed:25490919).
CC {ECO:0000250|UniProtKB:P23511, ECO:0000269|PubMed:25490919}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25490919}.
CC -!- TISSUE SPECIFICITY: Ubiquitous (PubMed:11250072, PubMed:9662544).
CC Expressed in seedlings, roots, petioles, hypocotyls, reproductive organ
CC tissues and leaves (PubMed:25490919). {ECO:0000269|PubMed:11250072,
CC ECO:0000269|PubMed:25490919, ECO:0000269|PubMed:9662544}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, first expressed in petioles and leaf
CC blades of the cotyledons as well as tops and bottoms of the hypocotyls.
CC {ECO:0000269|PubMed:25490919}.
CC -!- SIMILARITY: Belongs to the NFYA/HAP2 subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00966}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y13721; CAA74049.1; -; mRNA.
DR EMBL; AC011620; AAF26128.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74280.1; -; Genomic_DNA.
DR RefSeq; NP_187220.1; NM_111443.4.
DR AlphaFoldDB; Q9M9X4; -.
DR BioGRID; 5073; 17.
DR IntAct; Q9M9X4; 7.
DR STRING; 3702.AT3G05690.1; -.
DR PaxDb; Q9M9X4; -.
DR EnsemblPlants; AT3G05690.1; AT3G05690.1; AT3G05690.
DR GeneID; 819738; -.
DR Gramene; AT3G05690.1; AT3G05690.1; AT3G05690.
DR KEGG; ath:AT3G05690; -.
DR Araport; AT3G05690; -.
DR TAIR; locus:2078072; AT3G05690.
DR eggNOG; KOG1561; Eukaryota.
DR HOGENOM; CLU_065504_0_0_1; -.
DR InParanoid; Q9M9X4; -.
DR PhylomeDB; Q9M9X4; -.
DR PRO; PR:Q9M9X4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M9X4; baseline and differential.
DR Genevisible; Q9M9X4; AT.
DR GO; GO:0016602; C:CCAAT-binding factor complex; ISS:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:TAIR.
DR GO; GO:0009408; P:response to heat; IDA:UniProtKB.
DR InterPro; IPR001289; NFYA.
DR PANTHER; PTHR12632; PTHR12632; 1.
DR Pfam; PF02045; CBFB_NFYA; 1.
DR PRINTS; PR00616; CCAATSUBUNTB.
DR SMART; SM00521; CBF; 1.
DR PROSITE; PS51152; NFYA_HAP2_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..295
FT /note="Nuclear transcription factor Y subunit A-2"
FT /id="PRO_0000198772"
FT DNA_BIND 173..198
FT /note="NFYA/HAP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT REGION 178..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 139..165
FT /note="Subunit association domain (SAD)"
FT COMPBIAS 194..244
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 51
FT /note="G -> R (in Ref. 1; CAA74049)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 295 AA; 32139 MW; 9AA7BE76B0F0EBD8 CRC64;
MAMQTVREGL FSAPQTSWWT AFGSQPLAPE SLAGDSDSFA GVKVGSVGET GQRVDKQSNS
ATHLAFSLGD VKSPRLVPKP HGATFSMQSP CLELGFSQPP IYTKYPYGEQ QYYGVVSAYG
SQSRVMLPLN METEDSTIYV NSKQYHGIIR RRQSRAKAAA VLDQKKLSSR CRKPYMHHSR
HLHALRRPRG SGGRFLNTKS QNLENSGTNA KKGDGSMQIQ SQPKPQQSNS QNSEVVHPEN
GTMNLSNGLN VSGSEVTSMN YFLSSPVHSL GGMVMPSKWI AAAAAMDNGC CNFKT
