NFYA2_CAEEL
ID NFYA2_CAEEL Reviewed; 281 AA.
AC Q5ZEP9; F3Y5S2; F3Y5S3;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Nuclear transcription factor Y subunit nfya-2 {ECO:0000305};
DE AltName: Full=CAAT box DNA-binding protein subunit nfya-2 {ECO:0000305};
GN Name=nfya-2 {ECO:0000312|WormBase:Y53H1A.5a};
GN ORFNames=Y53H1A.5 {ECO:0000312|WormBase:Y53H1A.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=15704008; DOI=10.1007/s10735-004-6017-6;
RA Franchini A., Imbriano C., Peruzzi E., Mantovani R., Ottaviani E.;
RT "Expression of the CCAAT-binding factor NF-Y in Caenorhabditis elegans.";
RL J. Mol. Histol. 36:139-145(2005).
RN [3] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN NF-Y COMPLEX, INTERACTION WITH NFYB-1 AND
RP NFYC-2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=17574230; DOI=10.1016/j.ydbio.2007.05.021;
RA Deng H., Sun Y., Zhang Y., Luo X., Hou W., Yan L., Chen Y., Tian E.,
RA Han J., Zhang H.;
RT "Transcription factor NFY globally represses the expression of the C.
RT elegans Hox gene Abdominal-B homolog egl-5.";
RL Dev. Biol. 308:583-592(2007).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23933492; DOI=10.1016/j.ydbio.2013.08.001;
RA Milton A.C., Packard A.V., Clary L., Okkema P.G.;
RT "The NF-Y complex negatively regulates Caenorhabditis elegans tbx-2
RT expression.";
RL Dev. Biol. 382:38-47(2013).
CC -!- FUNCTION: Component of the sequence-specific heterotrimeric
CC transcription factor (nfya-2-NF-Y) which specifically recognizes a 5'-
CC CCAAT-3' box motif found in the promoters of its target genes to
CC regulate their expression and control cellular identity in particular
CC tissue types (PubMed:17574230). In association with the components in
CC the nfya-2-NF-Y complex, may repress the expression of the T-box
CC transcription factor tbx-2 throughout larval development, which most
CC likely restricts its expression to certain tissues (PubMed:23933492).
CC {ECO:0000269|PubMed:17574230, ECO:0000269|PubMed:23933492}.
CC -!- SUBUNIT: Forms a heterotrimeric transcription factor complex (nfya-2-
CC NF-Y complex) composed of nfya-2, nfyb-1 and nfyc-1 (PubMed:17574230).
CC Interacts with the nfyb-1 and nfyc-1 dimer; the interaction is required
CC for subsequent binding to the 5'-CCAAT-3' box motif in DNA
CC (PubMed:17574230). Does not interact with either nfyb-1 or nfyc-1 in
CC their monomeric form (PubMed:17574230). {ECO:0000269|PubMed:17574230}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17574230}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:Y53H1A.5a};
CC IsoId=Q5ZEP9-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y53H1A.5b};
CC IsoId=Q5ZEP9-2; Sequence=VSP_060605;
CC Name=c {ECO:0000312|WormBase:Y53H1A.5c};
CC IsoId=Q5ZEP9-3; Sequence=VSP_060604;
CC -!- TISSUE SPECIFICITY: Highly expressed in certain parts of the gonads
CC (PubMed:15704008). Expressed in the spermatheca, intestine and in some
CC neurons in the head (PubMed:17574230). Not expressed in the intestine,
CC the hypodermis, body wall muscle surrounding the pseudocoelomic space,
CC secretory cells in the pharyngeal terminal bulb wall, in the small
CC ganglia surrounding the pharynx and in the neurons running anteriorly
CC to the sensory organs in the head (PubMed:15704008).
CC {ECO:0000269|PubMed:15704008, ECO:0000269|PubMed:17574230}.
CC -!- DEVELOPMENTAL STAGE: Expressed at all developmental stages
CC (PubMed:17574230). Highly expressed in the intestine at all
CC developmental stages (PubMed:17574230). {ECO:0000269|PubMed:17574230}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in weak ectopic
CC expression of tbx-2 in seam cells of the head in young adults.
CC {ECO:0000269|PubMed:23933492}.
CC -!- SIMILARITY: Belongs to the NFYA/HAP2 subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00966}.
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DR EMBL; BX284601; CAH60767.2; -; Genomic_DNA.
DR EMBL; BX284601; CCA65672.1; -; Genomic_DNA.
DR EMBL; BX284601; CCA65673.1; -; Genomic_DNA.
DR RefSeq; NP_001251586.1; NM_001264657.1. [Q5ZEP9-1]
DR RefSeq; NP_001251587.1; NM_001264658.1. [Q5ZEP9-2]
DR RefSeq; NP_001251588.1; NM_001264659.1. [Q5ZEP9-3]
DR AlphaFoldDB; Q5ZEP9; -.
DR SMR; Q5ZEP9; -.
DR ComplexPortal; CPX-5667; CCAAT-binding factor complex, nfya-2 variant.
DR STRING; 6239.Y53H1A.5a; -.
DR PaxDb; Q5ZEP9; -.
DR EnsemblMetazoa; Y53H1A.5a.1; Y53H1A.5a.1; WBGene00043056. [Q5ZEP9-1]
DR EnsemblMetazoa; Y53H1A.5b.1; Y53H1A.5b.1; WBGene00043056. [Q5ZEP9-2]
DR EnsemblMetazoa; Y53H1A.5c.1; Y53H1A.5c.1; WBGene00043056. [Q5ZEP9-3]
DR GeneID; 3565955; -.
DR KEGG; cel:CELE_Y53H1A.5; -.
DR UCSC; Y53H1A.5; c. elegans. [Q5ZEP9-1]
DR CTD; 3565955; -.
DR WormBase; Y53H1A.5a; CE41285; WBGene00043056; nfya-2. [Q5ZEP9-1]
DR WormBase; Y53H1A.5b; CE37572; WBGene00043056; nfya-2. [Q5ZEP9-2]
DR WormBase; Y53H1A.5c; CE46100; WBGene00043056; nfya-2. [Q5ZEP9-3]
DR eggNOG; KOG1561; Eukaryota.
DR GeneTree; ENSGT00390000015714; -.
DR HOGENOM; CLU_991207_0_0_1; -.
DR InParanoid; Q5ZEP9; -.
DR OrthoDB; 1269806at2759; -.
DR PRO; PR:Q5ZEP9; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00043056; Expressed in pharyngeal muscle cell (C elegans) and 11 other tissues.
DR ExpressionAtlas; Q5ZEP9; baseline and differential.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IPI:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IMP:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009888; P:tissue development; IMP:UniProtKB.
DR InterPro; IPR001289; NFYA.
DR PANTHER; PTHR12632; PTHR12632; 1.
DR Pfam; PF02045; CBFB_NFYA; 1.
DR PRINTS; PR00616; CCAATSUBUNTB.
DR SMART; SM00521; CBF; 1.
DR PROSITE; PS51152; NFYA_HAP2_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..281
FT /note="Nuclear transcription factor Y subunit nfya-2"
FT /id="PRO_0000450333"
FT DNA_BIND 180..204
FT /note="NFYA/HAP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 163..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 150..173
FT /note="Subunit association domain (SAD)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT COMPBIAS 163..185
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..149
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060604"
FT VAR_SEQ 1..9
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060605"
SQ SEQUENCE 281 AA; 31913 MW; 8A7B56125484CDCD CRC64;
MNPRGNPSKM PTQIVLNRRP AAPARPQTTF TSLSQYHMYN TASTSSAPPP PPNQNFGQIR
EAIRTTQPTT VQLPPDCKLV EYTWQENGES RTVLIPMSND STEDDVRSAL PQSVLESINQ
HQNQLRQGNA QAVHYYQKHQ NPISHEKPIM VNPRQYKRII KRREMRQKME DSGRLPLERQ
KYMHESRRQH ALKRRRTGGR FDANAEAAAA SSEPSISSAA PSPPKAPRAP TTYAMIRPAT
SHPPVNIPKA PAMTYRPVQE EKKSIERISK AQMFTIPKRQ Q
