NFYA3_ARATH
ID NFYA3_ARATH Reviewed; 340 AA.
AC Q93ZH2; O23632; Q9SSP3;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 2.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Nuclear transcription factor Y subunit A-3;
DE Short=AtNF-YA-3;
DE AltName: Full=Transcriptional activator HAP2C;
GN Name=NFYA3; Synonyms=HAP2C; OrderedLocusNames=At1g72830;
GN ORFNames=F28P22.32, F3N23.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=9662544; DOI=10.1104/pp.117.3.1015;
RA Edwards D., Murray J.A.H., Smith A.G.;
RT "Multiple genes encoding the conserved CCAAT-box transcription factor
RT complex are expressed in Arabidopsis.";
RL Plant Physiol. 117:1015-1022(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=11250072; DOI=10.1016/s0378-1119(01)00323-7;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana.";
RL Gene 264:173-185(2001).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
CC -!- FUNCTION: Stimulates the transcription of various genes by recognizing
CC and binding to a CCAAT motif in promoters. {ECO:0000250}.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and
CC dimerize for NF-YA association and DNA binding (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q93ZH2-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:11250072,
CC ECO:0000269|PubMed:9662544}.
CC -!- SIMILARITY: Belongs to the NFYA/HAP2 subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00966}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL09779.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Y13722; CAA74050.1; -; mRNA.
DR EMBL; AC008017; AAD55630.1; -; Genomic_DNA.
DR EMBL; AC010926; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE35378.1; -; Genomic_DNA.
DR EMBL; AY057539; AAL09779.1; ALT_FRAME; mRNA.
DR EMBL; AY140028; AAM98169.1; -; mRNA.
DR EMBL; BT008825; AAP68264.1; -; mRNA.
DR PIR; C96753; C96753.
DR RefSeq; NP_565049.1; NM_105941.8. [Q93ZH2-1]
DR AlphaFoldDB; Q93ZH2; -.
DR BioGRID; 28833; 9.
DR IntAct; Q93ZH2; 2.
DR STRING; 3702.AT1G72830.2; -.
DR PaxDb; Q93ZH2; -.
DR EnsemblPlants; AT1G72830.1; AT1G72830.1; AT1G72830. [Q93ZH2-1]
DR GeneID; 843614; -.
DR Gramene; AT1G72830.1; AT1G72830.1; AT1G72830. [Q93ZH2-1]
DR KEGG; ath:AT1G72830; -.
DR Araport; AT1G72830; -.
DR eggNOG; KOG1561; Eukaryota.
DR InParanoid; Q93ZH2; -.
DR PhylomeDB; Q93ZH2; -.
DR PRO; PR:Q93ZH2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q93ZH2; baseline and differential.
DR Genevisible; Q93ZH2; AT.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IEA:InterPro.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR018362; CCAAT-binding_factor_CS.
DR InterPro; IPR001289; NFYA.
DR PANTHER; PTHR12632; PTHR12632; 1.
DR Pfam; PF02045; CBFB_NFYA; 1.
DR PRINTS; PR00616; CCAATSUBUNTB.
DR SMART; SM00521; CBF; 1.
DR PROSITE; PS00686; NFYA_HAP2_1; 1.
DR PROSITE; PS51152; NFYA_HAP2_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..340
FT /note="Nuclear transcription factor Y subunit A-3"
FT /id="PRO_0000198773"
FT DNA_BIND 212..237
FT /note="NFYA/HAP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT REGION 43..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 182..205
FT /note="Subunit association domain (SAD)"
FT COMPBIAS 50..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 40
FT /note="S -> P (in Ref. 1; CAA74050)"
FT /evidence="ECO:0000305"
FT CONFLICT 340
FT /note="I -> M (in Ref. 4; AAL09779)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 37618 MW; 34F989707EAA37B5 CRC64;
MMHQMLNKKD SATHSTLPYL NTSISWGVVP TDSVANRRGS AESLSLKVDS RPGHIQTTKQ
ISFQDQDSSS TQSTGQSYTE VASSGDDNPS RQISFSAKSG SEITQRKGFA SNPKQGSMTG
FPNIHFAPAQ ANFSFHYADP HYGGLLAATY LPQAPTCNPQ MVSMIPGRVP LPAELTETDP
VFVNAKQYHA IMRRRQQRAK LEAQNKLIRA RKPYLHESRH VHALKRPRGS GGRFLNTKKL
LQESEQAAAR EQEQDKLGQQ VNRKTNMSRF EAHMLQNNKD RSSTTSGSDI TSVSDGADIF
GHTEFQFSGF PTPINRAMLV HGQSNDMHGG GDMHHFSVHI
