NFYA7_ARATH
ID NFYA7_ARATH Reviewed; 190 AA.
AC Q84JP1; Q9S9P9;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Nuclear transcription factor Y subunit A-7;
DE Short=AtNF-YA-7;
GN Name=NFYA7; OrderedLocusNames=At1g30500; ORFNames=F26G16.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
CC -!- FUNCTION: Stimulates the transcription of various genes by recognizing
CC and binding to a CCAAT motif in promoters. {ECO:0000250}.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and
CC dimerize for NF-YA association and DNA binding (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q84JP1; Q9ZVC9: FRS3; NbExp=3; IntAct=EBI-4444640, EBI-4448729;
CC Q84JP1; O24410: IAA20; NbExp=3; IntAct=EBI-4444640, EBI-632272;
CC Q84JP1; Q38827: IAA9; NbExp=3; IntAct=EBI-4444640, EBI-632216;
CC Q84JP1; P46639: KNAT1; NbExp=3; IntAct=EBI-4444640, EBI-530486;
CC Q84JP1; Q9FNZ4: NIMIN-3; NbExp=3; IntAct=EBI-4444640, EBI-541115;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84JP1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84JP1-2; Sequence=VSP_016047;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NFYA/HAP2 subunit family.
CC {ECO:0000255|PROSITE-ProRule:PRU00966}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF19754.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC009917; AAF19754.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31234.1; -; Genomic_DNA.
DR EMBL; BT004274; AAO42275.1; -; mRNA.
DR EMBL; BT005561; AAO63981.1; -; mRNA.
DR PIR; A86430; A86430.
DR RefSeq; NP_849733.1; NM_179402.4. [Q84JP1-1]
DR AlphaFoldDB; Q84JP1; -.
DR SMR; Q84JP1; -.
DR BioGRID; 25164; 23.
DR IntAct; Q84JP1; 18.
DR STRING; 3702.AT1G30500.2; -.
DR PaxDb; Q84JP1; -.
DR EnsemblPlants; AT1G30500.2; AT1G30500.2; AT1G30500. [Q84JP1-1]
DR GeneID; 839929; -.
DR Gramene; AT1G30500.2; AT1G30500.2; AT1G30500. [Q84JP1-1]
DR KEGG; ath:AT1G30500; -.
DR Araport; AT1G30500; -.
DR TAIR; locus:2028150; AT1G30500.
DR eggNOG; KOG1561; Eukaryota.
DR InParanoid; Q84JP1; -.
DR OMA; VAYPYIG; -.
DR OrthoDB; 1411373at2759; -.
DR PhylomeDB; Q84JP1; -.
DR PRO; PR:Q84JP1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84JP1; baseline and differential.
DR Genevisible; Q84JP1; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR001289; NFYA.
DR PANTHER; PTHR12632; PTHR12632; 1.
DR Pfam; PF02045; CBFB_NFYA; 1.
DR PRINTS; PR00616; CCAATSUBUNTB.
DR SMART; SM00521; CBF; 1.
DR PROSITE; PS51152; NFYA_HAP2_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..190
FT /note="Nuclear transcription factor Y subunit A-7"
FT /id="PRO_0000198777"
FT DNA_BIND 133..158
FT /note="NFYA/HAP2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00966"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 103..126
FT /note="Subunit association domain (SAD)"
FT COMPBIAS 154..174
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 78..81
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_016047"
SQ SEQUENCE 190 AA; 21300 MW; 847A719B22DF24E4 CRC64;
MTSSIHELSD NIGSHEKQEQ RDSHFQPPIP SARNYESIVT SLVYSDPGTT NSMAPGQYPY
PDPYYRSIFA PPPQPYTGVH LQLMGVQQQG VPLPSDAVEE PVFVNAKQYH GILRRRQSRA
RLESQNKVIK SRKPYLHESR HLHAIRRPRG CGGRFLNAKK EDEHHEDSSH EEKSNLSAGK
SAMAASSGTS
