NFYB3_ARATH
ID NFYB3_ARATH Reviewed; 161 AA.
AC O23310;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Nuclear transcription factor Y subunit B-3 {ECO:0000303|PubMed:11250072, ECO:0000303|PubMed:11867211};
DE Short=AtNF-YB-3 {ECO:0000303|PubMed:11250072, ECO:0000303|PubMed:11867211};
DE Short=AtNF-YB3 {ECO:0000303|PubMed:25490919};
DE AltName: Full=Transcriptional activator HAP3C;
GN Name=NFYB3 {ECO:0000303|PubMed:11250072, ECO:0000303|PubMed:11867211};
GN Synonyms=HAP3C;
GN OrderedLocusNames=At4g14540 {ECO:0000312|Araport:AT4G14540};
GN ORFNames=dl3310w {ECO:0000312|EMBL:CAB10233.1},
GN FCAALL.252 {ECO:0000312|EMBL:CAB78496.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=11250072; DOI=10.1016/s0378-1119(01)00323-7;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana.";
RL Gene 264:173-185(2001).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
RN [8]
RP FUNCTION, SUBUNIT, INTERACTION WITH DPB3-1, INDUCTION BY HEAT STRESS AND
RP DEHYDRATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=25490919; DOI=10.1105/tpc.114.132928;
RA Sato H., Mizoi J., Tanaka H., Maruyama K., Qin F., Osakabe Y., Morimoto K.,
RA Ohori T., Kusakabe K., Nagata M., Shinozaki K., Yamaguchi-Shinozaki K.;
RT "Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat
RT stress-induced gene expression by forming a heat stress-specific
RT transcriptional complex with NF-Y subunits.";
RL Plant Cell 26:4954-4973(2014).
RN [9]
RP REVIEW.
RX PubMed=28119722; DOI=10.3389/fpls.2016.02045;
RA Zhao H., Wu D., Kong F., Lin K., Zhang H., Li G.;
RT "The Arabidopsis thaliana Nuclear Factor Y Transcription Factors.";
RL Front. Plant Sci. 7:2045-2045(2016).
CC -!- FUNCTION: Component of the NF-Y/HAP transcription factor complex (By
CC similarity). The NF-Y complex stimulates the transcription of various
CC genes by recognizing and binding to a CCAAT motif in promoters (By
CC similarity). Promotes the expression of heat stress-inducible genes by
CC contributing to the formation of a heat stress-specific transcriptional
CC complex with NF-Y subunits (e.g. DPB3-1, NF-YA2 and NF-YB3) and DREB2A
CC at the promoter of target genes, thus promoting heat tolerance
CC (PubMed:25490919). {ECO:0000250|UniProtKB:Q84W66,
CC ECO:0000269|PubMed:25490919}.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC (By similarity). NF-YB and NF-YC
CC must interact and dimerize for NF-YA association and DNA binding (By
CC similarity). Component of a heat stress-inducible transcriptional
CC complex with NF-YA and NF-YB subunits made, at least, of NFYA2, NFYB3
CC and DPB3-1 in cooperation with DREB2A (PubMed:25490919). Binds directly
CC with DPB3-1 (PubMed:25490919). {ECO:0000250|UniProtKB:P23511,
CC ECO:0000269|PubMed:25490919}.
CC -!- INTERACTION:
CC O23310; Q9LN09: NF-YC10; NbExp=4; IntAct=EBI-4452064, EBI-4461992;
CC O23310; Q9LHG0: NF-YC11; NbExp=5; IntAct=EBI-4452064, EBI-4452389;
CC O23310; Q8VY64: NFYA4; NbExp=3; IntAct=EBI-4452064, EBI-4461713;
CC O23310; Q58CM8: NFYC10; NbExp=3; IntAct=EBI-4452064, EBI-15191737;
CC O23310; Q4PSE2: NFYC8; NbExp=3; IntAct=EBI-4452064, EBI-15191571;
CC O23310; Q8L4B2: NFYC9; NbExp=3; IntAct=EBI-4452064, EBI-2466050;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25490919}. Cytoplasm,
CC cytosol {ECO:0000269|PubMed:25490919}. Note=Present in both cytoplasm
CC and nucleus under nonstress conditions but translocates in the nucleus
CC upon heat stress. {ECO:0000269|PubMed:25490919}.
CC -!- TISSUE SPECIFICITY: Ubiquitous (PubMed:11250072). Expressed in
CC seedlings, petioles, hypocotyls, reproductive organ tissues and leaves
CC (PubMed:25490919). {ECO:0000269|PubMed:11250072,
CC ECO:0000269|PubMed:25490919}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, first expressed in petioles and leaf
CC blades of the cotyledons as well as tops and bottoms of the hypocotyls.
CC {ECO:0000269|PubMed:25490919}.
CC -!- INDUCTION: Enhanced by heat stress but repressed by dehydration stress.
CC {ECO:0000269|PubMed:25490919}.
CC -!- SIMILARITY: Belongs to the NFYB/HAP3 subunit family. {ECO:0000305}.
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DR EMBL; Z97336; CAB10233.1; -; Genomic_DNA.
DR EMBL; AL161539; CAB78496.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83458.1; -; Genomic_DNA.
DR EMBL; AK117818; BAC42460.1; -; mRNA.
DR EMBL; BT003684; AAO39912.1; -; mRNA.
DR PIR; G71407; G71407.
DR RefSeq; NP_193190.1; NM_117534.3.
DR PDB; 7CVO; X-ray; 2.60 A; B/G=18-120.
DR PDBsum; 7CVO; -.
DR AlphaFoldDB; O23310; -.
DR SMR; O23310; -.
DR BioGRID; 12398; 22.
DR IntAct; O23310; 14.
DR STRING; 3702.AT4G14540.1; -.
DR PaxDb; O23310; -.
DR PRIDE; O23310; -.
DR ProteomicsDB; 248932; -.
DR EnsemblPlants; AT4G14540.1; AT4G14540.1; AT4G14540.
DR GeneID; 827101; -.
DR Gramene; AT4G14540.1; AT4G14540.1; AT4G14540.
DR KEGG; ath:AT4G14540; -.
DR Araport; AT4G14540; -.
DR TAIR; locus:2129885; AT4G14540.
DR eggNOG; KOG0869; Eukaryota.
DR HOGENOM; CLU_066247_1_1_1; -.
DR InParanoid; O23310; -.
DR OMA; AQNAGNS; -.
DR OrthoDB; 1529111at2759; -.
DR PhylomeDB; O23310; -.
DR PRO; PR:O23310; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O23310; baseline and differential.
DR Genevisible; O23310; AT.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IDA:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR027113; Transc_fact_NFYB/HAP3.
DR InterPro; IPR003956; Transcrpt_fac_NFYB/HAP3_CS.
DR PANTHER; PTHR11064; PTHR11064; 1.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00685; NFYB_HAP3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Activator; Cytoplasm; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9SLG0"
FT CHAIN 2..161
FT /note="Nuclear transcription factor Y subunit B-3"
FT /id="PRO_0000204617"
FT DNA_BIND 26..32
FT /evidence="ECO:0000250|UniProtKB:P13434"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 53..64
FT /note="Subunit association domain (SAD)"
FT /evidence="ECO:0000250"
FT REGION 114..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9SLG0"
FT HELIX 28..37
FT /evidence="ECO:0007829|PDB:7CVO"
FT HELIX 47..74
FT /evidence="ECO:0007829|PDB:7CVO"
FT HELIX 82..92
FT /evidence="ECO:0007829|PDB:7CVO"
FT HELIX 95..108
FT /evidence="ECO:0007829|PDB:7CVO"
SQ SEQUENCE 161 AA; 17186 MW; 5C452E20D98A7AF4 CRC64;
MADSDNDSGG HKDGGNASTR EQDRFLPIAN VSRIMKKALP ANAKISKDAK ETVQECVSEF
ISFITGEASD KCQREKRKTI NGDDLLWAMT TLGFEDYVEP LKVYLQKYRE VEGEKTTTAG
RQGDKEGGGG GGGAGSGSGG APMYGGGMVT TMGHQFSHHF S
