NFYB4_ARATH
ID NFYB4_ARATH Reviewed; 139 AA.
AC O04027; A0JPZ3;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Nuclear transcription factor Y subunit B-4;
DE Short=AtNF-YB-4;
DE AltName: Full=Transcriptional activator HAP3D;
GN Name=NFYB4; Synonyms=HAP3D; OrderedLocusNames=At1g09030; ORFNames=F7G19.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11250072; DOI=10.1016/s0378-1119(01)00323-7;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana.";
RL Gene 264:173-185(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
CC -!- FUNCTION: Component of the NF-Y/HAP transcription factor complex. The
CC NF-Y complex stimulates the transcription of various genes by
CC recognizing and binding to a CCAAT motif in promoters.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and
CC dimerize for NF-YA association and DNA binding (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC O04027; Q58CM8: NFYC10; NbExp=3; IntAct=EBI-1751677, EBI-15191737;
CC O04027; Q4PSE2: NFYC8; NbExp=3; IntAct=EBI-1751677, EBI-15191571;
CC O04027; Q8L4B2: NFYC9; NbExp=3; IntAct=EBI-1751677, EBI-2466050;
CC O04027; Q9LX49: PRN1; NbExp=2; IntAct=EBI-1751677, EBI-1606661;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, siliques and young rosettes.
CC {ECO:0000269|PubMed:11250072}.
CC -!- SIMILARITY: Belongs to the NFYB/HAP3 subunit family. {ECO:0000305}.
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DR EMBL; AC000106; AAB70405.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28385.1; -; Genomic_DNA.
DR EMBL; BT029363; ABK32177.1; -; mRNA.
DR PIR; C86222; C86222.
DR RefSeq; NP_172377.1; NM_100774.2.
DR AlphaFoldDB; O04027; -.
DR SMR; O04027; -.
DR BioGRID; 22665; 27.
DR IntAct; O04027; 22.
DR STRING; 3702.AT1G09030.1; -.
DR PaxDb; O04027; -.
DR PRIDE; O04027; -.
DR EnsemblPlants; AT1G09030.1; AT1G09030.1; AT1G09030.
DR GeneID; 837424; -.
DR Gramene; AT1G09030.1; AT1G09030.1; AT1G09030.
DR KEGG; ath:AT1G09030; -.
DR Araport; AT1G09030; -.
DR TAIR; locus:2036064; AT1G09030.
DR eggNOG; KOG0869; Eukaryota.
DR HOGENOM; CLU_066247_12_2_1; -.
DR InParanoid; O04027; -.
DR OMA; CDYEIKE; -.
DR OrthoDB; 1529111at2759; -.
DR PhylomeDB; O04027; -.
DR PRO; PR:O04027; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O04027; baseline.
DR Genevisible; O04027; AT.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR027113; Transc_fact_NFYB/HAP3.
DR InterPro; IPR003956; Transcrpt_fac_NFYB/HAP3_CS.
DR PANTHER; PTHR11064; PTHR11064; 1.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00685; NFYB_HAP3; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..139
FT /note="Nuclear transcription factor Y subunit B-4"
FT /id="PRO_0000204618"
FT DNA_BIND 8..14
FT /evidence="ECO:0000250"
FT REGION 35..46
FT /note="Subunit association domain (SAD)"
FT /evidence="ECO:0000250"
FT REGION 90..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..113
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 139 AA; 15741 MW; 3AF2DD2F0F1DE748 CRC64;
MTDEDRLLPI ANVGRLMKQI LPSNAKISKE AKQTVQECAT EFISFVTCEA SEKCHRENRK
TVNGDDIWWA LSTLGLDNYA DAVGRHLHKY REAERERTEH NKGSNDSGNE KETNTRSDVQ
NQSTKFIRVV EKGSSSSAR
