NFYB8_ARATH
ID NFYB8_ARATH Reviewed; 173 AA.
AC Q8VYK4; Q9ZQC3;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Nuclear transcription factor Y subunit B-8;
DE Short=AtNF-YB-8;
GN Name=NFYB8; OrderedLocusNames=At2g37060; ORFNames=T2N18.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11250072; DOI=10.1016/s0378-1119(01)00323-7;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana.";
RL Gene 264:173-185(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11867211; DOI=10.1016/s0378-1119(01)00833-2;
RA Gusmaroli G., Tonelli C., Mantovani R.;
RT "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding
RT nuclear factor Y subunits.";
RL Gene 283:41-48(2002).
CC -!- FUNCTION: Component of the NF-Y/HAP transcription factor complex. The
CC NF-Y complex stimulates the transcription of various genes by
CC recognizing and binding to a CCAAT motif in promoters.
CC -!- SUBUNIT: Heterotrimeric transcription factor composed of three
CC components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and
CC dimerize for NF-YA association and DNA binding (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q8VYK4; Q58CM8: NFYC10; NbExp=3; IntAct=EBI-15192579, EBI-15191737;
CC Q8VYK4; Q9FGP8: NFYC7; NbExp=3; IntAct=EBI-15192579, EBI-2466133;
CC Q8VYK4; Q4PSE2: NFYC8; NbExp=3; IntAct=EBI-15192579, EBI-15191571;
CC Q8VYK4; Q8L4B2: NFYC9; NbExp=3; IntAct=EBI-15192579, EBI-2466050;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers and mature rosettes.
CC {ECO:0000269|PubMed:11250072}.
CC -!- SIMILARITY: Belongs to the NFYB/HAP3 subunit family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD18153.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006260; AAD18153.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09345.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09346.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09347.1; -; Genomic_DNA.
DR EMBL; AY070477; AAL49943.1; -; mRNA.
DR EMBL; AY091673; AAM10272.1; -; mRNA.
DR PIR; A84788; A84788.
DR RefSeq; NP_001031500.1; NM_001036423.1.
DR RefSeq; NP_850277.2; NM_179946.4.
DR RefSeq; NP_973617.1; NM_201888.3.
DR AlphaFoldDB; Q8VYK4; -.
DR SMR; Q8VYK4; -.
DR BioGRID; 3626; 16.
DR IntAct; Q8VYK4; 9.
DR STRING; 3702.AT2G37060.3; -.
DR iPTMnet; Q8VYK4; -.
DR PaxDb; Q8VYK4; -.
DR PRIDE; Q8VYK4; -.
DR EnsemblPlants; AT2G37060.1; AT2G37060.1; AT2G37060.
DR EnsemblPlants; AT2G37060.2; AT2G37060.2; AT2G37060.
DR EnsemblPlants; AT2G37060.3; AT2G37060.3; AT2G37060.
DR GeneID; 818282; -.
DR Gramene; AT2G37060.1; AT2G37060.1; AT2G37060.
DR Gramene; AT2G37060.2; AT2G37060.2; AT2G37060.
DR Gramene; AT2G37060.3; AT2G37060.3; AT2G37060.
DR KEGG; ath:AT2G37060; -.
DR Araport; AT2G37060; -.
DR TAIR; locus:2061789; AT2G37060.
DR eggNOG; KOG0869; Eukaryota.
DR HOGENOM; CLU_066247_13_1_1; -.
DR InParanoid; Q8VYK4; -.
DR OMA; YREVMIT; -.
DR OrthoDB; 1529111at2759; -.
DR PhylomeDB; Q8VYK4; -.
DR PRO; PR:Q8VYK4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VYK4; baseline and differential.
DR Genevisible; Q8VYK4; AT.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR027113; Transc_fact_NFYB/HAP3.
DR InterPro; IPR003956; Transcrpt_fac_NFYB/HAP3_CS.
DR PANTHER; PTHR11064; PTHR11064; 2.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00685; NFYB_HAP3; 1.
PE 1: Evidence at protein level;
KW Acetylation; Activator; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9SLG0"
FT CHAIN 2..173
FT /note="Nuclear transcription factor Y subunit B-8"
FT /id="PRO_0000204622"
FT DNA_BIND 35..41
FT /evidence="ECO:0000250"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..73
FT /note="Subunit association domain (SAD)"
FT /evidence="ECO:0000250"
FT REGION 123..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9SLG0"
SQ SEQUENCE 173 AA; 18998 MW; 8C01E135B2DEC36A CRC64;
MAESQAKSPG GCGSHESGGD QSPRSLHVRE QDRFLPIANI SRIMKRGLPA NGKIAKDAKE
IVQECVSEFI SFVTSEASDK CQREKRKTIN GDDLLWAMAT LGFEDYMEPL KVYLMRYREM
EGDTKGSAKG GDPNAKKDGQ SSQNGQFSQL AHQGPYGNSQ AQQHMMVPMP GTD
