NFYC1_CAEEL
ID NFYC1_CAEEL Reviewed; 232 AA.
AC O17072; A0A0K3AWV9;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Nuclear transcription factor Y subunit nfyc-1 {ECO:0000305};
DE AltName: Full=CAAT box DNA-binding protein subunit nfyc-1 {ECO:0000305};
GN Name=nfyc-1 {ECO:0000312|WormBase:F23F1.1a};
GN ORFNames=F23F1.1 {ECO:0000312|WormBase:F23F1.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15704008; DOI=10.1007/s10735-004-6017-6;
RA Franchini A., Imbriano C., Peruzzi E., Mantovani R., Ottaviani E.;
RT "Expression of the CCAAT-binding factor NF-Y in Caenorhabditis elegans.";
RL J. Mol. Histol. 36:139-145(2005).
RN [3] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN NF-Y COMPLEX, INTERACTION WITH NFYB-1; NFYA-1
RP AND NFYA-2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17574230; DOI=10.1016/j.ydbio.2007.05.021;
RA Deng H., Sun Y., Zhang Y., Luo X., Hou W., Yan L., Chen Y., Tian E.,
RA Han J., Zhang H.;
RT "Transcription factor NFY globally represses the expression of the C.
RT elegans Hox gene Abdominal-B homolog egl-5.";
RL Dev. Biol. 308:583-592(2007).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23933492; DOI=10.1016/j.ydbio.2013.08.001;
RA Milton A.C., Packard A.V., Clary L., Okkema P.G.;
RT "The NF-Y complex negatively regulates Caenorhabditis elegans tbx-2
RT expression.";
RL Dev. Biol. 382:38-47(2013).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=25873636; DOI=10.1534/g3.115.018101;
RA Milton A.C., Okkema P.G.;
RT "Caenorhabditis elegans TBX-2 Directly Regulates Its Own Expression in a
RT Negative Autoregulatory Loop.";
RL G3 (Bethesda) 5:1177-1186(2015).
CC -!- FUNCTION: Component of sequence-specific heterotrimeric transcription
CC factor (nfya-1-NF-Y and nfya-2-NF-Y) complexes which specifically
CC recognize a 5'-CCAAT-3' box motif found in the promoters of its target
CC genes to regulate their expression and control cellular identity in
CC particular tissue types (PubMed:17574230). In association with the
CC components in the NF-Y complexes, represses the expression of the T-box
CC transcription factor tbx-2 throughout larval development, which most
CC likely restricts its expression to certain tissues (PubMed:23933492).
CC May act to repress txb-2 expression in conjunction with tbx-2 itself,
CC which has an autoregulatory role (Probable). In association with the
CC components in the nfya-1-NF-Y complex, negatively regulates the
CC expression of the homeobox protein egl-5 to spatially restrict its
CC expression in tissues such as the head (PubMed:17574230). May regulate
CC egl-5 expression in association with the mes-2-mes-3-mes-6 complex
CC (PubMed:17574230). {ECO:0000269|PubMed:17574230,
CC ECO:0000269|PubMed:23933492, ECO:0000305|PubMed:25873636}.
CC -!- SUBUNIT: Forms two NF-Y heterotrimeric transcription factor complexes:
CC the nfya-1-NF-Y complex is composed of nfya-1, nfyb-1 and nfyc-1, and
CC the nfya-2-NF-Y complex is composed of nfya-2, nfyb-1 and nfyc-1
CC (PubMed:17574230). Interacts with nfyb-1; the interaction is direct and
CC is required for the interaction with either nfya-1 or nfya-2, and
CC subsequent binding of the complex to the 5'-CCAAT-3' box motif in DNA
CC (PubMed:17574230). {ECO:0000269|PubMed:17574230}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17574230}. Cytoplasm
CC {ECO:0000269|PubMed:15704008, ECO:0000269|PubMed:17574230}. Perikaryon
CC {ECO:0000269|PubMed:15704008}. Note=Localizes to the cytoplasm of
CC secretory cells and cell bodies of the small ganglia surrounding the
CC pharynx. {ECO:0000269|PubMed:15704008}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:F23F1.1a};
CC IsoId=O17072-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F23F1.1b};
CC IsoId=O17072-2; Sequence=VSP_060607;
CC -!- TISSUE SPECIFICITY: Expressed in certain parts of the gonads with high
CC expression in fertilized oocytes in the uterus and mature oocytes from
CC the distal to the proximal arm of the gonad, but weak expression in the
CC syncytial ovaries and immature oocytes at the beginning of the proximal
CC arm of the gonad (PubMed:15704008). Expressed in the excretory cell,
CC secretory cells in the pharyngeal terminal bulb wall, in the small
CC ganglia surrounding the pharynx and in the neurons running anteriorly
CC to the sensory organs in the head (PubMed:15704008). Not expressed in
CC the intestine, the hypodermis or body wall muscle surrounding the
CC pseudocoelomic space (PubMed:15704008). {ECO:0000269|PubMed:15704008}.
CC -!- DEVELOPMENTAL STAGE: Expressed in cells of the developing embryo
CC (PubMed:15704008, PubMed:17574230). At the larval stages, weakly
CC expressed in neurons and pharyngeal secretory cells (PubMed:15704008,
CC PubMed:17574230). At larval stages also expressed in the developing
CC hermaphrodite vulva and male tail (PubMed:17574230). Not expressed in
CC the gonads in larval stages (PubMed:15704008).
CC {ECO:0000269|PubMed:15704008, ECO:0000269|PubMed:17574230}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in ectopic
CC expression of the homeobox protein egl-5 in the head region
CC (PubMed:17574230). RNAi-mediated knockdown results in ectopic
CC expression of tbx-2 in the gut and seam cells of L4 stage larvae and
CC adults (PubMed:23933492). {ECO:0000269|PubMed:17574230,
CC ECO:0000269|PubMed:23933492}.
CC -!- SIMILARITY: Belongs to the NFYC/HAP5 subunit family. {ECO:0000305}.
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DR EMBL; BX284602; CCD62352.1; -; Genomic_DNA.
DR EMBL; BX284602; CTQ86476.1; -; Genomic_DNA.
DR RefSeq; NP_001300537.1; NM_001313608.1. [O17072-2]
DR RefSeq; NP_493645.1; NM_061244.6. [O17072-1]
DR AlphaFoldDB; O17072; -.
DR SMR; O17072; -.
DR ComplexPortal; CPX-5666; CCAAT-binding factor complex, nfya-1 variant.
DR ComplexPortal; CPX-5667; CCAAT-binding factor complex, nfya-2 variant.
DR IntAct; O17072; 12.
DR STRING; 6239.F23F1.1; -.
DR PaxDb; O17072; -.
DR PeptideAtlas; O17072; -.
DR EnsemblMetazoa; F23F1.1a.1; F23F1.1a.1; WBGene00017742. [O17072-1]
DR EnsemblMetazoa; F23F1.1b.1; F23F1.1b.1; WBGene00017742. [O17072-2]
DR GeneID; 173385; -.
DR KEGG; cel:CELE_F23F1.1; -.
DR UCSC; F23F1.1; c. elegans. [O17072-1]
DR CTD; 173385; -.
DR WormBase; F23F1.1a; CE27996; WBGene00017742; nfyc-1. [O17072-1]
DR WormBase; F23F1.1b; CE50568; WBGene00017742; nfyc-1. [O17072-2]
DR eggNOG; KOG1657; Eukaryota.
DR GeneTree; ENSGT00940000155689; -.
DR HOGENOM; CLU_1195785_0_0_1; -.
DR InParanoid; O17072; -.
DR OrthoDB; 1558176at2759; -.
DR PhylomeDB; O17072; -.
DR SignaLink; O17072; -.
DR PRO; PR:O17072; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00017742; Expressed in germ line (C elegans) and 4 other tissues.
DR ExpressionAtlas; O17072; baseline and differential.
DR GO; GO:0016602; C:CCAAT-binding factor complex; IPI:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IMP:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009888; P:tissue development; IMP:UniProtKB.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR027170; HAP5_su.
DR InterPro; IPR009072; Histone-fold.
DR PANTHER; PTHR10252:SF8; PTHR10252:SF8; 1.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; DNA-binding; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..232
FT /note="Nuclear transcription factor Y subunit nfyc-1"
FT /id="PRO_0000450335"
FT REGION 191..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..226
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..35
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060607"
SQ SEQUENCE 232 AA; 26722 MW; 1C752127AA023D4B CRC64;
MSQFPEVLDN NLLHAENNET AEYIQNDGTN QSEVFMEHPY TPNMHPINMP SIVEGAVHPY
NNLIHHNDAI PPAKYASMRQ MTEDFWREKK QKMTEISEED MLNKSKNMSV PMARVKKIMR
IDDDVRNFMI ASDAPIFMAQ AAEFFIEEMT AMGWQYVSEA RRRILQKADI ASAVQKSDQF
DFLIDFLPPK TVPTTSTNGP GHMSEDSFQD PNMHSDFHQR TSNSSVNRSH HN
