NGA1_ARATH
ID NGA1_ARATH Reviewed; 310 AA.
AC O82799;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=B3 domain-containing transcription factor NGA1;
DE AltName: Full=Protein NGATHA 1;
GN Name=NGA1; OrderedLocusNames=At2g46870; ORFNames=F19D11.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RX PubMed=16603651; DOI=10.1105/tpc.105.040725;
RA Alvarez J.P., Pekker I., Goldshmidt A., Blum E., Amsellem Z., Eshed Y.;
RT "Endogenous and synthetic microRNAs stimulate simultaneous, efficient, and
RT localized regulation of multiple targets in diverse species.";
RL Plant Cell 18:1134-1151(2006).
RN [6]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
RN [7]
RP INTERACTION WITH BRX.
RX PubMed=19465596; DOI=10.1242/dev.035444;
RA Scacchi E., Osmont K.S., Beuchat J., Salinas P., Navarrete-Gomez M.,
RA Trigueros M., Ferrandiz C., Hardtke C.S.;
RT "Dynamic, auxin-responsive plasma membrane-to-nucleus movement of
RT Arabidopsis BRX.";
RL Development 136:2059-2067(2009).
RN [8]
RP INTERACTION WITH BZIP30.
RX PubMed=27402171; DOI=10.1111/tpj.13264;
RA Lozano-Sotomayor P., Chavez Montes R.A., Silvestre-Vano M.,
RA Herrera-Ubaldo H., Greco R., Pablo-Villa J., Galliani B.M.,
RA Diaz-Ramirez D., Weemen M., Boutilier K., Pereira A., Colombo L.,
RA Madueno F., Marsch-Martinez N., de Folter S.;
RT "Altered expression of the bZIP transcription factor DRINK ME affects
RT growth and reproductive development in Arabidopsis thaliana.";
RL Plant J. 88:437-451(2016).
CC -!- FUNCTION: Regulates lateral organ growth. Functionally redundant with
CC NGA2, NGA3 and NGA4. {ECO:0000269|PubMed:16603651}.
CC -!- SUBUNIT: Interacts with BRX. Interacts with BZIP30 (PubMed:27402171).
CC {ECO:0000269|PubMed:19465596, ECO:0000269|PubMed:27402171}.
CC -!- INTERACTION:
CC O82799; Q17TI5: BRX; NbExp=6; IntAct=EBI-4427936, EBI-4426649;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; AC004411; AAC34233.1; -; Genomic_DNA.
DR EMBL; AC005310; AAM15018.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10765.1; -; Genomic_DNA.
DR EMBL; AK118045; BAC42676.1; -; mRNA.
DR EMBL; BT008534; AAP40361.1; -; mRNA.
DR PIR; T02200; T02200.
DR RefSeq; NP_566089.1; NM_130254.4.
DR PDB; 5OS9; X-ray; 1.80 A; A/B=28-143.
DR PDBsum; 5OS9; -.
DR AlphaFoldDB; O82799; -.
DR SMR; O82799; -.
DR BioGRID; 4635; 6.
DR IntAct; O82799; 8.
DR STRING; 3702.AT2G46870.1; -.
DR PaxDb; O82799; -.
DR PRIDE; O82799; -.
DR ProteomicsDB; 251133; -.
DR EnsemblPlants; AT2G46870.1; AT2G46870.1; AT2G46870.
DR GeneID; 819300; -.
DR Gramene; AT2G46870.1; AT2G46870.1; AT2G46870.
DR KEGG; ath:AT2G46870; -.
DR Araport; AT2G46870; -.
DR TAIR; locus:2041404; AT2G46870.
DR eggNOG; ENOG502QSHQ; Eukaryota.
DR HOGENOM; CLU_038898_3_2_1; -.
DR InParanoid; O82799; -.
DR OMA; MECPNTT; -.
DR OrthoDB; 661086at2759; -.
DR PhylomeDB; O82799; -.
DR PRO; PR:O82799; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82799; baseline and differential.
DR Genevisible; O82799; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:1901371; P:regulation of leaf morphogenesis; IGI:TAIR.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR044800; LEC2-like.
DR PANTHER; PTHR31140; PTHR31140; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..310
FT /note="B3 domain-containing transcription factor NGA1"
FT /id="PRO_0000375091"
FT DNA_BIND 35..141
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 251..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..290
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..310
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 31..38
FT /evidence="ECO:0007829|PDB:5OS9"
FT HELIX 41..44
FT /evidence="ECO:0007829|PDB:5OS9"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:5OS9"
FT HELIX 54..60
FT /evidence="ECO:0007829|PDB:5OS9"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:5OS9"
FT STRAND 83..91
FT /evidence="ECO:0007829|PDB:5OS9"
FT TURN 92..95
FT /evidence="ECO:0007829|PDB:5OS9"
FT STRAND 96..101
FT /evidence="ECO:0007829|PDB:5OS9"
FT HELIX 102..110
FT /evidence="ECO:0007829|PDB:5OS9"
FT STRAND 117..123
FT /evidence="ECO:0007829|PDB:5OS9"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:5OS9"
SQ SEQUENCE 310 AA; 34890 MW; 2E667FC0B3798653 CRC64;
MMTDLSLTRD EDEEEAKPLA EEEGAREVAD REHMFDKVVT PSDVGKLNRL VIPKQHAERF
FPLDSSSNEK GLLLNFEDLT GKSWRFRYSY WNSSQSYVMT KGWSRFVKDK KLDAGDIVSF
QRCVGDSGRD SRLFIDWRRR PKVPDHPHFA AGAMFPRFYS FPSTNYSLYN HQQQRHHHSG
GGYNYHQIPR EFGYGYFVRS VDQRNNPAAA VADPLVIESV PVMMHGRANQ ELVGTAGKRL
RLFGVDMECG ESGMTNSTEE ESSSSGGSLP RGGGGGASSS SFFQLRLGSS SEDDHFTKKG
KSSLSFDLDQ
