ID   NGAP_DICDI              Reviewed;         877 AA.
AC   Q54Y08;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Probable Ras GTPase-activating-like protein ngap;
DE            Short=DdNGAP;
GN   Name=ngap; ORFNames=DDB_G0278483;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA   Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA   Ivens A., Martinez J.L., Escalante R.;
RT   "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT   and specific effects from PAO1 and PA14 strains.";
RL   BMC Microbiol. 8:109-109(2008).
CC   -!- FUNCTION: May function as a Ras GTPase-activating protein.
CC       {ECO:0000250}.
CC   -!- INDUCTION: Up-regulated by P.aeruginosa, PAO1 strain and PA14 strain
CC       infection. {ECO:0000269|PubMed:18590548}.
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DR   EMBL; AAFI02000023; EAL68414.1; -; Genomic_DNA.
DR   RefSeq; XP_642394.1; XM_637302.1.
DR   AlphaFoldDB; Q54Y08; -.
DR   SMR; Q54Y08; -.
DR   STRING; 44689.DDB0220496; -.
DR   PaxDb; Q54Y08; -.
DR   EnsemblProtists; EAL68414; EAL68414; DDB_G0278483.
DR   GeneID; 8621599; -.
DR   KEGG; ddi:DDB_G0278483; -.
DR   dictyBase; DDB_G0278483; ngap.
DR   eggNOG; KOG1032; Eukaryota.
DR   eggNOG; KOG2059; Eukaryota.
DR   HOGENOM; CLU_328024_0_0_1; -.
DR   InParanoid; Q54Y08; -.
DR   OMA; HPGIKTT; -.
DR   Reactome; R-DDI-5658442; Regulation of RAS by GAPs.
DR   PRO; PR:Q54Y08; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005813; C:centrosome; TAS:dictyBase.
DR   GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR   GO; GO:0031256; C:leading edge membrane; IDA:dictyBase.
DR   GO; GO:0005096; F:GTPase activator activity; IMP:dictyBase.
DR   GO; GO:0005543; F:phospholipid binding; IDA:dictyBase.
DR   GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:dictyBase.
DR   GO; GO:0043087; P:regulation of GTPase activity; IEA:InterPro.
DR   GO; GO:0051592; P:response to calcium ion; IMP:dictyBase.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 1.10.506.10; -; 2.
DR   Gene3D; 2.60.40.150; -; 1.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR039360; Ras_GTPase.
DR   InterPro; IPR023152; RasGAP_CS.
DR   InterPro; IPR001936; RasGAP_dom.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   PANTHER; PTHR10194; PTHR10194; 1.
DR   Pfam; PF00168; C2; 1.
DR   Pfam; PF00616; RasGAP; 2.
DR   SMART; SM00239; C2; 1.
DR   SMART; SM00323; RasGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   SUPFAM; SSF49562; SSF49562; 1.
DR   PROSITE; PS50004; C2; 1.
DR   PROSITE; PS00509; RAS_GTPASE_ACTIV_1; 1.
DR   PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; GTPase activation; Reference proteome.
FT   CHAIN           1..877
FT                   /note="Probable Ras GTPase-activating-like protein ngap"
FT                   /id="PRO_0000393333"
FT   DOMAIN          72..218
FT                   /note="C2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   DOMAIN          575..769
FT                   /note="Ras-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
FT   REGION          350..456
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          820..848
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        359..456
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   877 AA;  98378 MW;  67BF5A9BC3CD49E5 CRC64;
     MMESLTVNEN TINEFEIKND IIIEQQQIQS KTSIPLLNIK DGNTTTTNTI NEINNNNNNI
     ETSTMSRKSL MTPSATYESL IRQQKTKDCN LTVKVFEARN LIEARLRKRE MVKNGKSFKR
     AQNLLTEISS PNLMTFSDTT DPYCTVQLEK QKQRTRTIPK KLNPFWCEEF QLEISDPASA
     KLVLSVMDEK KYSNDEHIGK LVIPINTLKD QKERELWFPL TQPQSSKKVP QIQILFNFKP
     ISLTDPSQPG HIQWKVLFGR NLSHSLATPL PSMSAASMGG STSTENISNV YTNTTPFINW
     SVRSKKGDIV IDEEGIAWQD ALTNGITREL KDSIECITFT LWRYEPKTFS DDGDISGLKS
     PRGTNSSASG SGFIPSTPPP SSHQKSNDTT ATTTPSSTPS TPISPSSQSN NIKTPDSKTR
     SSSNASTNTP QTTPKSTGTS GGPAPPETIN LSSSINSTSS LISNGSINVI DSSNNLHHHH
     NNGTNIMNPL KDGYEQYFIG QGIVLASHID IEKPNDQWLC LYPKQTLDNK FGDIRLKLKY
     SEEVVLPLQS YQPLLELLQQ ENLYTITLLG KVTKHRESVS NNLIRVFEKT GKCLYLLKSL
     TDHEIDSTNN PDIIFRGNSL ATKSVDLFMK LIGIPYLSQT IGPLIKKIYS SKKSCEIDPT
     KLEKGEDIKK NCKNLLSWVK KMTTAILSSV NNCPGPLREV FKSIQDKVVQ RYPKDEITRY
     TAVSGFIFLR FFCPAILAPK LFDLMPDHPG IKTTRSLILI AKTLQNLANQ VEFGEYKEDF
     MKDMNRFVID NMENMKSFIN TLSTVPADCP PGALQSPIIL EKELACLYRH LIKQRQDMAE
     EMESTESEPK TQQEKDSFGK LIKILNSLDE DVQLASN