ID   NGCF_STRCO              Reviewed;         353 AA.
AC   O50500;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Diacetylchitobiose uptake system permease protein NgcF {ECO:0000305};
GN   Name=ngcF {ECO:0000303|PubMed:30089751};
GN   OrderedLocusNames=SCO6006 {ECO:0000312|EMBL:CAA15783.1};
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   FUNCTION, SUBUNIT, AND INDUCTION.
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=30089751; DOI=10.1264/jsme2.me17172;
RA   Iinuma C., Saito A., Ohnuma T., Tenconi E., Rosu A., Colson S.,
RA   Mizutani Y., Liu F., Swiatek-Polatynska M., van Wezel G.P., Rigali S.,
RA   Fujii T., Miyashita K.;
RT   "NgcESco acts as a lower-affinity binding protein of an ABC transporter for
RT   the uptake of N,N'-diacetylchitobiose in Streptomyces coelicolor A3(2).";
RL   Microbes Environ. 33:272-281(2018).
CC   -!- FUNCTION: Part of the ABC transporter complex NgcEFG-MsiK involved in
CC       N,N'-diacetylchitobiose ((GlcNAc)2) uptake. Responsible for the
CC       translocation of the substrate across the membrane.
CC       {ECO:0000305|PubMed:30089751}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MsiK),
CC       two transmembrane proteins (NgcF and NgcG) and a solute-binding protein
CC       (NgcE). {ECO:0000305|PubMed:30089751}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Constitutively expressed. Slightly induced by GlcNAc,
CC       (GlcNAc)2 and chitin. Repressed by DasR. {ECO:0000269|PubMed:30089751}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. {ECO:0000305}.
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DR   EMBL; AL939126; CAA15783.1; -; Genomic_DNA.
DR   PIR; T35671; T35671.
DR   RefSeq; NP_630120.1; NC_003888.3.
DR   RefSeq; WP_011030592.1; NZ_VNID01000007.1.
DR   AlphaFoldDB; O50500; -.
DR   SMR; O50500; -.
DR   STRING; 100226.SCO6006; -.
DR   GeneID; 1101448; -.
DR   KEGG; sco:SCO6006; -.
DR   PATRIC; fig|100226.15.peg.6104; -.
DR   eggNOG; COG1175; Bacteria.
DR   HOGENOM; CLU_016047_0_0_11; -.
DR   OMA; AYQIVYF; -.
DR   PhylomeDB; O50500; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..353
FT                   /note="Diacetylchitobiose uptake system permease protein
FT                   NgcF"
FT                   /id="PRO_0000447876"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        303..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          95..320
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          329..353
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   353 AA;  38014 MW;  D9818A86A42F8B57 CRC64;
     MKDTIPTAET ASRRPEPAAR GGRPRRRKLT FDRVTFFLAF LGVPLAIFVI FVLIPFGQAI
     FWGMTDWRGF SPDYNFVGFD NFTKMFQDDI FLKALRNVAL LAAFVPLVTL TLALGVAVAI
     TLGGPSKGPV RGIRGASFYR IISFFPYVVP AIIVGLIWAQ MYDPNAGLLN GVLTGLGLDQ
     FDTFAWLGEK AAAMPAVMFV IVWGLVGFYA VLFIAAIKGV PGELYEAAKI DGAGRFRTTI
     SITLPAIRDS VQTAYIYLGI AALDAFVYVQ AMVPNGGPDN STLTISQRLF NVAFAKQQFG
     YATAMGVVLA AVTLVFAALV FLVNRLTGGG EGESKRKAPG SRARRAAAKG GAR