NGDN_HUMAN
ID NGDN_HUMAN Reviewed; 315 AA.
AC Q8NEJ9; A8K760; Q9Y400;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Neuroguidin;
DE AltName: Full=Centromere accumulated nuclear protein 1;
DE Short=CANu1;
DE AltName: Full=EIF4E-binding protein;
GN Name=NGDN; Synonyms=C14orf120;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Skeletal muscle;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=18547334; DOI=10.1111/j.1365-2443.2008.01205.x;
RA Sihn C.R., Lee Y.S., Jeong J.S., Park K., Kim S.H.;
RT "CANu1, a novel nucleolar protein, accumulated on centromere in response to
RT DNA damage.";
RL Genes Cells 13:787-796(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND SER-143, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121; SER-142; SER-143;
RP SER-204 AND SER-214, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Involved in the translational repression of cytoplasmic
CC polyadenylation element (CPE)-containing mRNAs. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CPEB1 and EIF4E. {ECO:0000250}.
CC -!- INTERACTION:
CC Q8NEJ9; Q9NY61: AATF; NbExp=4; IntAct=EBI-9995414, EBI-372428;
CC Q8NEJ9; P67870: CSNK2B; NbExp=3; IntAct=EBI-9995414, EBI-348169;
CC Q8NEJ9; P55081: MFAP1; NbExp=3; IntAct=EBI-9995414, EBI-1048159;
CC Q8NEJ9; Q5C9Z4: NOM1; NbExp=3; IntAct=EBI-9995414, EBI-2685618;
CC Q8NEJ9; A0A142I5B9; Xeno; NbExp=2; IntAct=EBI-9995414, EBI-20625235;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:18547334}. Chromosome, centromere
CC {ECO:0000269|PubMed:18547334}. Cytoplasm {ECO:0000250}. Cell
CC projection, axon {ECO:0000250}. Cell projection, dendrite
CC {ECO:0000250}. Cell projection, filopodium {ECO:0000250}. Note=Detected
CC in axons, dendrites and filopodia. Colocalized with EIF4E in neurites
CC (By similarity). Translocated from nucleolus to nuclear foci in
CC response to UV damage. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8NEJ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NEJ9-2; Sequence=VSP_009935, VSP_009936;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SAS10 family. {ECO:0000305}.
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DR EMBL; AK291875; BAF84564.1; -; mRNA.
DR EMBL; AL050003; CAB43232.2; -; mRNA.
DR EMBL; CH471078; EAW66149.1; -; Genomic_DNA.
DR EMBL; BC030817; AAH30817.1; -; mRNA.
DR CCDS; CCDS32051.1; -. [Q8NEJ9-2]
DR CCDS; CCDS41926.1; -. [Q8NEJ9-1]
DR PIR; T08694; T08694.
DR RefSeq; NP_001036100.1; NM_001042635.1. [Q8NEJ9-1]
DR RefSeq; NP_056329.1; NM_015514.1. [Q8NEJ9-2]
DR PDB; 7MQ8; EM; 3.60 A; NC=1-315.
DR PDBsum; 7MQ8; -.
DR AlphaFoldDB; Q8NEJ9; -.
DR SMR; Q8NEJ9; -.
DR BioGRID; 117466; 110.
DR CORUM; Q8NEJ9; -.
DR IntAct; Q8NEJ9; 34.
DR MINT; Q8NEJ9; -.
DR STRING; 9606.ENSP00000386134; -.
DR iPTMnet; Q8NEJ9; -.
DR PhosphoSitePlus; Q8NEJ9; -.
DR BioMuta; NGDN; -.
DR DMDM; 46395905; -.
DR EPD; Q8NEJ9; -.
DR jPOST; Q8NEJ9; -.
DR MassIVE; Q8NEJ9; -.
DR MaxQB; Q8NEJ9; -.
DR PaxDb; Q8NEJ9; -.
DR PeptideAtlas; Q8NEJ9; -.
DR PRIDE; Q8NEJ9; -.
DR ProteomicsDB; 73169; -. [Q8NEJ9-1]
DR ProteomicsDB; 73170; -. [Q8NEJ9-2]
DR TopDownProteomics; Q8NEJ9-1; -. [Q8NEJ9-1]
DR Antibodypedia; 89; 170 antibodies from 26 providers.
DR DNASU; 25983; -.
DR Ensembl; ENST00000397154.7; ENSP00000380340.3; ENSG00000129460.16. [Q8NEJ9-2]
DR Ensembl; ENST00000408901.8; ENSP00000386134.3; ENSG00000129460.16. [Q8NEJ9-1]
DR GeneID; 25983; -.
DR KEGG; hsa:25983; -.
DR MANE-Select; ENST00000408901.8; ENSP00000386134.3; NM_001042635.2; NP_001036100.1.
DR UCSC; uc001wjy.4; human. [Q8NEJ9-1]
DR CTD; 25983; -.
DR DisGeNET; 25983; -.
DR GeneCards; NGDN; -.
DR HGNC; HGNC:20271; NGDN.
DR HPA; ENSG00000129460; Low tissue specificity.
DR MIM; 610777; gene.
DR neXtProt; NX_Q8NEJ9; -.
DR OpenTargets; ENSG00000129460; -.
DR PharmGKB; PA134957933; -.
DR VEuPathDB; HostDB:ENSG00000129460; -.
DR eggNOG; KOG3117; Eukaryota.
DR GeneTree; ENSGT00500000044922; -.
DR HOGENOM; CLU_031901_0_0_1; -.
DR InParanoid; Q8NEJ9; -.
DR OMA; GYGGEEW; -.
DR OrthoDB; 1511802at2759; -.
DR PhylomeDB; Q8NEJ9; -.
DR TreeFam; TF313713; -.
DR PathwayCommons; Q8NEJ9; -.
DR SignaLink; Q8NEJ9; -.
DR BioGRID-ORCS; 25983; 441 hits in 1091 CRISPR screens.
DR ChiTaRS; NGDN; human.
DR GenomeRNAi; 25983; -.
DR Pharos; Q8NEJ9; Tbio.
DR PRO; PR:Q8NEJ9; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q8NEJ9; protein.
DR Bgee; ENSG00000129460; Expressed in oocyte and 208 other tissues.
DR ExpressionAtlas; Q8NEJ9; baseline and differential.
DR Genevisible; Q8NEJ9; HS.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR GO; GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR InterPro; IPR007146; Sas10/Utp3/C1D.
DR PANTHER; PTHR13237; PTHR13237; 1.
DR Pfam; PF04000; Sas10_Utp3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Cell projection;
KW Centromere; Chromosome; Coiled coil; Cytoplasm; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Translation regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19413330"
FT CHAIN 2..315
FT /note="Neuroguidin"
FT /id="PRO_0000114331"
FT REGION 41..174
FT /note="Necessary for interaction with EIF4E"
FT /evidence="ECO:0000250"
FT REGION 124..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 277..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 13..41
FT /evidence="ECO:0000255"
FT COILED 181..205
FT /evidence="ECO:0000255"
FT COMPBIAS 299..315
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:19413330"
FT MOD_RES 121
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 204
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 311
FT /note="F -> Q (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_009935"
FT VAR_SEQ 312..315
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_009936"
FT VARIANT 15
FT /note="V -> L (in dbSNP:rs10149626)"
FT /id="VAR_051898"
FT VARIANT 308
FT /note="K -> I (in dbSNP:rs17093050)"
FT /id="VAR_051899"
SQ SEQUENCE 315 AA; 35894 MW; F5B26C3C2EDDC840 CRC64;
MAALGVLESD LPSAVTLLKN LQEQVMAVTA QVKSLTQKVQ AGAYPTEKGL SFLEVKDQLL
LMYLMDLTHL ILDKASGGSL QGHDAVLRLV EIRTVLEKLR PLDQKLKYQI DKLIKTAVTG
SLSENDPLRF KPHPSNMMSK LSSEDEEEDE AEDDQSEASG KKSVKGVSKK YVPPRLVPVH
YDETEAEREK KRLERAKRRA LSSSVIRELK EQYSDAPEEI RDARHPHVTR QSQEDQHRIN
YEESMMVRLS VSKREKGRRK RANVMSSQLH SLTHFSDISA LTGGTVHLDE DQNPIKKRKK
IPQKGRKKKG FRRRR
