NGDN_MOUSE
ID NGDN_MOUSE Reviewed; 315 AA.
AC Q9DB96; Q8CIJ2;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Neuroguidin;
DE AltName: Full=EIF4E-binding protein;
GN Name=Ngdn; Synonyms=Ngd;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH CPEB1 AND EIF4E, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=16705177; DOI=10.1128/mcb.02470-05;
RA Jung M.-Y., Lorenz L., Richter J.D.;
RT "Translational control by neuroguidin, a eukaryotic initiation factor 4E
RT and CPEB binding protein.";
RL Mol. Cell. Biol. 26:4277-4287(2006).
CC -!- FUNCTION: Inhibits mRNA translation in a cytoplasmic polyadenylation
CC element (CPE)-dependent manner. {ECO:0000269|PubMed:16705177}.
CC -!- SUBUNIT: Interacts with CPEB1 and EIF4E. {ECO:0000269|PubMed:16705177}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16705177}. Nucleus,
CC nucleolus {ECO:0000250}. Chromosome, centromere {ECO:0000250}.
CC Cytoplasm {ECO:0000269|PubMed:16705177}. Cell projection, axon
CC {ECO:0000269|PubMed:16705177}. Cell projection, dendrite
CC {ECO:0000269|PubMed:16705177}. Cell projection, filopodium
CC {ECO:0000269|PubMed:16705177}. Note=Translocated from nucleolus to
CC nuclear foci in response to UV damage (By similarity). Detected in
CC axons, dendrites and filopodia. Colocalized with EIF4E in neurites.
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in testis, ovary, spleen, kidney,
CC hippocampus and cerebellum (at protein level). Expressed in testis,
CC ovary, spleen, kidney, brain. {ECO:0000269|PubMed:16705177}.
CC -!- SIMILARITY: Belongs to the SAS10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH23770.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK005098; BAB23816.1; -; mRNA.
DR EMBL; BC023770; AAH23770.1; ALT_INIT; mRNA.
DR EMBL; BC052790; AAH52790.1; -; mRNA.
DR CCDS; CCDS36928.1; -.
DR RefSeq; NP_081166.1; NM_026890.2.
DR AlphaFoldDB; Q9DB96; -.
DR SMR; Q9DB96; -.
DR BioGRID; 213145; 2.
DR STRING; 10090.ENSMUSP00000022815; -.
DR iPTMnet; Q9DB96; -.
DR PhosphoSitePlus; Q9DB96; -.
DR EPD; Q9DB96; -.
DR MaxQB; Q9DB96; -.
DR PaxDb; Q9DB96; -.
DR PeptideAtlas; Q9DB96; -.
DR PRIDE; Q9DB96; -.
DR ProteomicsDB; 252960; -.
DR Antibodypedia; 89; 170 antibodies from 26 providers.
DR Ensembl; ENSMUST00000022815; ENSMUSP00000022815; ENSMUSG00000022204.
DR GeneID; 68966; -.
DR KEGG; mmu:68966; -.
DR UCSC; uc007txx.2; mouse.
DR CTD; 25983; -.
DR MGI; MGI:1916216; Ngdn.
DR VEuPathDB; HostDB:ENSMUSG00000022204; -.
DR eggNOG; KOG3117; Eukaryota.
DR GeneTree; ENSGT00500000044922; -.
DR HOGENOM; CLU_031901_0_0_1; -.
DR InParanoid; Q9DB96; -.
DR OMA; GYGGEEW; -.
DR OrthoDB; 1511802at2759; -.
DR PhylomeDB; Q9DB96; -.
DR TreeFam; TF313713; -.
DR BioGRID-ORCS; 68966; 26 hits in 75 CRISPR screens.
DR ChiTaRS; Ngdn; mouse.
DR PRO; PR:Q9DB96; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q9DB96; protein.
DR Bgee; ENSMUSG00000022204; Expressed in paneth cell and 261 other tissues.
DR Genevisible; Q9DB96; MM.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR GO; GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR InterPro; IPR007146; Sas10/Utp3/C1D.
DR PANTHER; PTHR13237; PTHR13237; 1.
DR Pfam; PF04000; Sas10_Utp3; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell projection; Centromere; Chromosome; Coiled coil;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor;
KW Translation regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT CHAIN 2..315
FT /note="Neuroguidin"
FT /id="PRO_0000114332"
FT REGION 41..174
FT /note="Necessary for interaction with EIF4E"
FT /evidence="ECO:0000269|PubMed:16705177"
FT REGION 123..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 212..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 7..41
FT /evidence="ECO:0000255"
FT COILED 181..203
FT /evidence="ECO:0000255"
FT COMPBIAS 298..315
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT MOD_RES 121
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT MOD_RES 204
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEJ9"
FT CONFLICT 154
FT /note="G -> D (in Ref. 2; AAH23770)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 315 AA; 35659 MW; A5C59A3949A0F40C CRC64;
MAAPEVLESD VSSSITLLKN LQEQVMAVTA QIQALTTKVR AGTYSTEKGL SFLEVKDQLL
LMYLMDLSHL ILDKASGASL QGHPAVLRLV EIRTVLEKLR PLDQKLKYQI DKLVKTAVTG
SLSENDPLRF KPHPSNMVSK LSSEDEEESE AEEGQSEASG KKSAKGSAKK YVPPRLVPVH
YDETEAEREQ KRLEKAKRRA LSSSVIRELK EQYSDAPEEI RDARHPHVTR QSQEDQHRVN
YEESMMVRLS VSKREKGLRR RASAMSSQLH SLTHFSDISA LTGGTAHLDE DQNPVKKRKK
LPKKGRKKKG FRRRW
