NGF_CHICK
ID NGF_CHICK Reviewed; 243 AA.
AC P05200;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Beta-nerve growth factor;
DE Short=Beta-NGF;
DE Flags: Precursor;
GN Name=NGF; Synonyms=NGFB;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3017695; DOI=10.1002/j.1460-2075.1986.tb04386.x;
RA Ebendahl T., Larhammar D., Persson H.;
RT "Structure and expression of the chicken beta nerve growth factor gene.";
RL EMBO J. 5:1483-1487(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 118-243.
RX PubMed=3720959; DOI=10.1016/0014-5793(86)81441-7;
RA Wion D., Perret C., Frechin N., Keller A., Behar G., Brachet P.,
RA Auffray C.;
RT "Molecular cloning of the avian beta-nerve growth factor gene:
RT transcription in brain.";
RL FEBS Lett. 203:82-86(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 121-243.
RX PubMed=2427334; DOI=10.1002/j.1460-2075.1986.tb04387.x;
RA Meier R., Becker-Andre M., Gotz R., Heumann R., Shaw A., Thoenen H.;
RT "Molecular cloning of bovine and chick nerve growth factor (NGF):
RT delineation of conserved and unconserved domains and their relationship to
RT the biological activity and antigenicity of NGF.";
RL EMBO J. 5:1489-1493(1986).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 181-222.
RX PubMed=2025430; DOI=10.1016/0896-6273(91)90180-8;
RA Hallboeoek F., Ibanez C.F., Persson H.;
RT "Evolutionary studies of the nerve growth factor family reveal a novel
RT member abundantly expressed in Xenopus ovary.";
RL Neuron 6:845-858(1991).
RN [5]
RP MUTAGENESIS OF TRP-145; TYR-176; TRP-200; TRP-223; ARG-224 AND ARG-227.
RX PubMed=2328722; DOI=10.1002/j.1460-2075.1990.tb08265.x;
RA Ibanez C.F., Hallboeoek F., Ebendal T., Persson H.;
RT "Structure-function studies of nerve growth factor: functional importance
RT of highly conserved amino acid residues.";
RL EMBO J. 9:1477-1483(1990).
CC -!- FUNCTION: Nerve growth factor is important for the development and
CC maintenance of the sympathetic and sensory nervous systems.
CC Extracellular ligand for the NTRK1 and NGFR receptors, activates
CC cellular signaling cascades through those receptor tyrosine kinase to
CC regulate neuronal proliferation, differentiation and survival.
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the NGF-beta family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA27633.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X04003; CAA27633.1; ALT_INIT; Genomic_DNA.
DR EMBL; X04067; CAA27703.1; -; Genomic_DNA.
DR EMBL; M26810; AAA48984.1; -; mRNA.
DR PIR; A26311; A26311.
DR AlphaFoldDB; P05200; -.
DR SMR; P05200; -.
DR STRING; 9031.ENSGALP00000004030; -.
DR PaxDb; P05200; -.
DR VEuPathDB; HostDB:geneid_396466; -.
DR eggNOG; ENOG502RYPU; Eukaryota.
DR HOGENOM; CLU_059942_1_1_1; -.
DR InParanoid; P05200; -.
DR PhylomeDB; P05200; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0008021; C:synaptic vesicle; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IBA:GO_Central.
DR GO; GO:0005163; F:nerve growth factor receptor binding; IBA:GO_Central.
DR GO; GO:0007613; P:memory; IBA:GO_Central.
DR GO; GO:0050804; P:modulation of chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IBA:GO_Central.
DR GO; GO:0021675; P:nerve development; IBA:GO_Central.
DR GO; GO:0038180; P:nerve growth factor signaling pathway; IBA:GO_Central.
DR GO; GO:0048812; P:neuron projection morphogenesis; IBA:GO_Central.
DR GO; GO:0007422; P:peripheral nervous system development; IBA:GO_Central.
DR GO; GO:0048672; P:positive regulation of collateral sprouting; IBA:GO_Central.
DR GO; GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IBA:GO_Central.
DR GO; GO:0045664; P:regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; IBA:GO_Central.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR020408; Nerve_growth_factor-like.
DR InterPro; IPR002072; Nerve_growth_factor-rel.
DR InterPro; IPR020425; Nerve_growth_factor_bsu.
DR InterPro; IPR019846; Nerve_growth_factor_CS.
DR PANTHER; PTHR11589; PTHR11589; 1.
DR Pfam; PF00243; NGF; 1.
DR PIRSF; PIRSF001789; NGF; 1.
DR PRINTS; PR00268; NGF.
DR PRINTS; PR01913; NGFBETA.
DR SMART; SM00140; NGF; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00248; NGF_1; 1.
DR PROSITE; PS50270; NGF_2; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Disulfide bond; Growth factor;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..125
FT /id="PRO_0000019611"
FT CHAIN 126..243
FT /note="Beta-nerve growth factor"
FT /id="PRO_0000019612"
FT REGION 26..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 139..204
FT /evidence="ECO:0000250"
FT DISULFID 182..232
FT /evidence="ECO:0000250"
FT DISULFID 192..234
FT /evidence="ECO:0000250"
FT MUTAGEN 145
FT /note="W->F: Diminishes receptor binding; when associated
FT with F-200 and F-223."
FT /evidence="ECO:0000269|PubMed:2328722"
FT MUTAGEN 176
FT /note="Y->F: No effect."
FT /evidence="ECO:0000269|PubMed:2328722"
FT MUTAGEN 200
FT /note="W->F: Diminishes receptor binding; when associated
FT with F-145 and F-223."
FT /evidence="ECO:0000269|PubMed:2328722"
FT MUTAGEN 223
FT /note="W->F: Diminishes receptor binding; when associated
FT with F-145 and F-200."
FT /evidence="ECO:0000269|PubMed:2328722"
FT MUTAGEN 224
FT /note="R->G: Impairs receptor binding; when associated with
FT G-227."
FT /evidence="ECO:0000269|PubMed:2328722"
FT MUTAGEN 224
FT /note="R->K: No effect."
FT /evidence="ECO:0000269|PubMed:2328722"
FT MUTAGEN 227
FT /note="R->G: Impairs receptor binding; when associated with
FT G-224."
FT /evidence="ECO:0000269|PubMed:2328722"
SQ SEQUENCE 243 AA; 27138 MW; 74C306CB2079DA07 CRC64;
MHSVMSMLYY TLIIAFLIGT QAAPKSEDNG PLEYPAEHSL PSTQQSNGQH IAKAAPQTTH
GRFAWMPDGT EDLNIAMDQN FFKKKRFRSS RVLFSTQPPP VSRKGQSTGF LSSAVSLNRT
ARTKRTAHPV LHRGEFSVCD SVSMWVGDKT TATDIKGKEV TVLGEVNINN NVFKQYFFET
KCRDPRPVSS GCRGIDAKHW NSYCTTTHTF VKALTMEGKQ AAWRFIRIDT ACVCVLSRKS
GRP
