NGN1_CAEEL
ID NGN1_CAEEL Reviewed; 184 AA.
AC Q95XG7;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Helix-loop-helix protein ngn-1 {ECO:0000305};
GN Name=ngn-1 {ECO:0000312|WormBase:Y69A2AR.29};
GN ORFNames=Y69A2AR.29 {ECO:0000312|WormBase:Y69A2AR.29};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH HLH-2, AND DEVELOPMENTAL STAGE.
RX PubMed=21041366; DOI=10.1242/dev.058834;
RA Nakano S., Ellis R.E., Horvitz H.R.;
RT "Otx-dependent expression of proneural bHLH genes establishes a neuronal
RT bilateral asymmetry in C. elegans.";
RL Development 137:4017-4027(2010).
RN [3] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=32273286; DOI=10.1534/g3.120.401126;
RA Christensen E.L., Beasley A., Radchuk J., Mielko Z.E., Preston E.,
RA Stuckett S., Murray J.I., Hudson M.L.;
RT "ngn-1/neurogenin Activates Transcription of Multiple Terminal Selector
RT Transcription Factors in the Caenorhabditis elegans Nervous System.";
RL G3 (Bethesda) 10:1949-1962(2020).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=32665354; DOI=10.1534/g3.120.401547;
RA Zhang A., Noma K., Yan D.;
RT "Regulation of Gliogenesis by lin-32/Atoh1 in Caenorhabditis elegans.";
RL G3 (Bethesda) 10:3271-3278(2020).
CC -!- FUNCTION: Acts as a transcriptional regulator (By similarity).
CC Regulates expression of various genes, including homeobox protein unc-
CC 42 and helix-loop-helix protein hlh-34 (PubMed:32273286). Required for
CC embryonic viability, neuromuscular development, organization of the
CC nerve ring and neuronal cell body location (PubMed:32273286). Regulates
CC AIY neuron axon morphology and cell fate (PubMed:32273286). Plays a
CC role in cell autonomously establishing a neuronal left-right asymmetry
CC (PubMed:21041366). Involved in regulating glial specification
CC (PubMed:32665354). {ECO:0000250|UniProtKB:Q9Y4Z2,
CC ECO:0000269|PubMed:21041366, ECO:0000269|PubMed:32273286,
CC ECO:0000269|PubMed:32665354}.
CC -!- SUBUNIT: Interacts with hlh-2; the interaction is direct.
CC {ECO:0000269|PubMed:21041366}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the early embryo in ABpra/p and
CC MSpa/p cells, with weaker expression in ABar descendants
CC (PubMed:32273286). Expressed left-right asymmetrically in the embryo,
CC in the mother cell to the MI pharyngeal motorneuron but not the mother
CC cell of the e3D epithelial cell (PubMed:21041366).
CC {ECO:0000269|PubMed:21041366, ECO:0000269|PubMed:32273286}.
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DR EMBL; BX284604; CCD74131.1; -; Genomic_DNA.
DR RefSeq; NP_500236.1; NM_067835.3.
DR AlphaFoldDB; Q95XG7; -.
DR SMR; Q95XG7; -.
DR IntAct; Q95XG7; 2.
DR STRING; 6239.Y69A2AR.29; -.
DR PaxDb; Q95XG7; -.
DR EnsemblMetazoa; Y69A2AR.29.1; Y69A2AR.29.1; WBGene00003595.
DR GeneID; 177045; -.
DR KEGG; cel:CELE_Y69A2AR.29; -.
DR UCSC; Y69A2AR.29; c. elegans.
DR CTD; 177045; -.
DR WormBase; Y69A2AR.29; CE26560; WBGene00003595; ngn-1.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000169303; -.
DR HOGENOM; CLU_115553_0_0_1; -.
DR InParanoid; Q95XG7; -.
DR OMA; QCCETGS; -.
DR OrthoDB; 1599854at2759; -.
DR PhylomeDB; Q95XG7; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00003595; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IMP:UniProtKB.
DR GO; GO:0061564; P:axon development; IMP:UniProtKB.
DR GO; GO:0014014; P:negative regulation of gliogenesis; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032666; TAP-like.
DR PANTHER; PTHR19290:SF134; PTHR19290:SF134; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..184
FT /note="Helix-loop-helix protein ngn-1"
FT /id="PRO_0000453175"
FT DOMAIN 62..114
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..75
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 76..114
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 164..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 184 AA; 21255 MW; DCD6EC3ABC24A8AB CRC64;
MYHHSPFYPH HLQTGEQDLD MERENDMDQN SKNSTQKPVK REKRRYRCRK RSPATIERAK
TVRRDKANAR ERRRMNSLND ALEHLRGILP ALPDEPKMTK IETLRKAQEY IASLSFQLSG
GSPDSSQCCE TGSCGLCSAS QSLQSTPFQS PCFPQPLQYP SSYSNPPSQM YYHHHHQSPS
FPHH
