NGN1_DANRE
ID NGN1_DANRE Reviewed; 208 AA.
AC O42606;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Neurogenin-1;
DE Short=NGN-1;
DE AltName: Full=Neurogenic differentiation factor 3;
DE Short=NeuroD3;
DE AltName: Full=Neurogenin-related protein 1;
GN Name=neurog1; Synonyms=neurod3, ngn1, ngr1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=9409673; DOI=10.1242/dev.124.22.4557;
RA Blader P., Fischer N., Gradwohl G., Guillemont F., Straehle U.;
RT "The activity of neurogenin1 is controlled by local cues in the zebrafish
RT embryo.";
RL Development 124:4557-4569(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Embryo;
RX PubMed=9469669; DOI=10.1016/s0304-3940(97)00908-7;
RA Kim C.-H., Bae Y.-K., Yamanaka Y., Yamashita S., Shimizu T., Fujii R.,
RA Park H.-C., Yeo S.-Y., Huh T.-L., Hibi M., Hirano T.;
RT "Overexpression of neurogenin induces ectopic expression of HuC in
RT zebrafish.";
RL Neurosci. Lett. 239:113-116(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=9733104;
RX DOI=10.1002/(sici)1097-0177(199809)213:1<92::aid-aja9>3.0.co;2-t;
RA Korzh V., Sleptsova I., Liao J., He J., Gong Z.;
RT "Expression of zebrafish bHLH genes ngn1 and nrd defines distinct stages of
RT neural differentiation.";
RL Dev. Dyn. 213:92-104(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Thisse B.;
RT "Danio rerio neurogenin the earliest marker of primary neurons starts to be
RT expressed at midgastrula.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12015292; DOI=10.1242/dev.129.11.2639;
RA Cornell R.A., Eisen J.S.;
RT "Delta/Notch signaling promotes formation of zebrafish neural crest by
RT repressing Neurogenin 1 function.";
RL Development 129:2639-2648(2002).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12413897; DOI=10.1006/dbio.2002.0820;
RA Andermann P., Ungos J., Raible D.W.;
RT "Neurogenin1 defines zebrafish cranial sensory ganglia precursors.";
RL Dev. Biol. 251:45-58(2002).
RN [8]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12702659; DOI=10.1242/dev.00452;
RA Cau E., Wilson S.W.;
RT "Ash1a and neurogenin1 function downstream of floating head to regulate
RT epiphysial neurogenesis.";
RL Development 130:2455-2466(2003).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15659486; DOI=10.1242/dev.01616;
RA Amoyel M., Cheng Y.-C., Jiang Y.-J., Wilkinson D.G.;
RT "Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary
RT cell specification in the zebrafish hindbrain.";
RL Development 132:775-785(2005).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16549779; DOI=10.1073/pnas.0600337103;
RA Jeong J.-Y., Einhorn Z., Mercurio S., Lee S., Lau B., Mione M.,
RA Wilson S.W., Guo S.;
RT "Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic
RT neurons and is regulated by the conserved zinc finger protein Tof/Fezl.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5143-5148(2006).
CC -!- FUNCTION: Transcriptional regulator. Activates transcription by binding
CC to the E box-containing promoter (By similarity). Mediates neuronal
CC differentiation. Required for the development of Rohon-Beard spinal
CC sensory neurons and dorsal root ganglion neurons, but not for primary
CC motoneurons or autonomic neurons. Required for development of all
CC cranial ganglia but not associated glial cells. Regulates epiphysial
CC neurogenesis, acting partially redundantly with ascl1a and downstream
CC of flh. Required for the development of basal forebrain dopaminergic
CC neurons; involved in the specification of dopaminergic progenitor
CC cells. May be involved in maintaining rhombomere boundaries in the
CC hindbrain. {ECO:0000250, ECO:0000269|PubMed:12015292,
CC ECO:0000269|PubMed:12413897, ECO:0000269|PubMed:12702659,
CC ECO:0000269|PubMed:15659486, ECO:0000269|PubMed:16549779,
CC ECO:0000269|PubMed:9409673, ECO:0000269|PubMed:9469669}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Embryonic nervous system. Expressed transiently in
CC neurogenic placodes prior to delamination and formation of cranial
CC ganglia. Expressed early (6-somite stage) in Rohon-Beard spinal sensory
CC neurons and later in neural crest-derived dorsal root ganglion neurons.
CC At 24 hours post-fertilization (hpf), expressed in specific regions of
CC the brain and spinal cord. In hindbrain, expressed in presumptive
CC neuroblasts adjacent to the rhombomere boundaries. In basal forebrain,
CC expressed in dopaminergic progenitor cells.
CC {ECO:0000269|PubMed:12015292, ECO:0000269|PubMed:12413897,
CC ECO:0000269|PubMed:15659486, ECO:0000269|PubMed:16549779,
CC ECO:0000269|PubMed:9409673, ECO:0000269|PubMed:9469669,
CC ECO:0000269|PubMed:9733104}.
CC -!- DEVELOPMENTAL STAGE: First detected at the late gastrulation stage (9
CC hpf). Strongly expressed in distinct domains in the neural plate at the
CC 3-somite stage. First expressed in the epiphysis at around 14 hpf.
CC {ECO:0000269|PubMed:12702659, ECO:0000269|PubMed:9409673,
CC ECO:0000269|PubMed:9469669, ECO:0000269|PubMed:9733104}.
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DR EMBL; AF017301; AAB70528.1; -; Genomic_DNA.
DR EMBL; AF024535; AAB82271.1; -; mRNA.
DR EMBL; AF036149; AAB88821.1; -; mRNA.
DR EMBL; U94588; AAD09254.1; -; mRNA.
DR EMBL; BC056575; AAH56575.1; -; mRNA.
DR EMBL; BC066427; AAH66427.1; -; mRNA.
DR RefSeq; NP_571116.1; NM_131041.1.
DR AlphaFoldDB; O42606; -.
DR SMR; O42606; -.
DR STRING; 7955.ENSDARP00000073024; -.
DR PaxDb; O42606; -.
DR Ensembl; ENSDART00000078563; ENSDARP00000073024; ENSDARG00000056130.
DR GeneID; 30239; -.
DR KEGG; dre:30239; -.
DR CTD; 4762; -.
DR ZFIN; ZDB-GENE-990415-174; neurog1.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000167363; -.
DR HOGENOM; CLU_097959_0_0_1; -.
DR InParanoid; O42606; -.
DR OMA; FAYGPGD; -.
DR OrthoDB; 1524429at2759; -.
DR PhylomeDB; O42606; -.
DR TreeFam; TF315153; -.
DR SignaLink; O42606; -.
DR PRO; PR:O42606; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 14.
DR Bgee; ENSDARG00000056130; Expressed in regional part of brain and 100 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070888; F:E-box binding; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048909; P:anterior lateral line nerve development; IMP:ZFIN.
DR GO; GO:0031103; P:axon regeneration; IMP:ZFIN.
DR GO; GO:0007413; P:axonal fasciculation; IMP:ZFIN.
DR GO; GO:0021516; P:dorsal spinal cord development; IMP:ZFIN.
DR GO; GO:0021884; P:forebrain neuron development; IDA:ZFIN.
DR GO; GO:0048699; P:generation of neurons; IMP:UniProtKB.
DR GO; GO:0021986; P:habenula development; IGI:ZFIN.
DR GO; GO:0021575; P:hindbrain morphogenesis; IGI:UniProtKB.
DR GO; GO:0050768; P:negative regulation of neurogenesis; IMP:ZFIN.
DR GO; GO:0007399; P:nervous system development; IMP:ZFIN.
DR GO; GO:0048884; P:neuromast development; IMP:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0021772; P:olfactory bulb development; IMP:ZFIN.
DR GO; GO:0071698; P:olfactory placode development; IMP:ZFIN.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:UniProtKB.
DR GO; GO:0048935; P:peripheral nervous system neuron development; IMP:ZFIN.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0048918; P:posterior lateral line nerve development; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032653; Ngn1.
DR PANTHER; PTHR19290:SF130; PTHR19290:SF130; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..208
FT /note="Neurogenin-1"
FT /id="PRO_0000127399"
FT DOMAIN 70..122
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..179
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 208 AA; 22911 MW; CDE333AE483DC51A CRC64;
MEIVYSDMET SSCDYSFSHT DDEDSRSSLH PASPASSCGK PPASPAGLQQ KKRRRGRARN
ETTVHVVKKN RRLKANDRER NRMHNLNDAL DALRSVLPAF PDDTKLTKIE TLRFAHNYIW
ALSETIRIAD QKQGKSRDGP LLLPGLSCMA DAPSPGSDSC SWPSGASSSS SSPSYCNSDP
GSPAAMDDFG YLQTDVVYSY RNFVPSIY
