NGN1_MOUSE
ID NGN1_MOUSE Reviewed; 244 AA.
AC P70660;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Neurogenin-1;
DE Short=NGN-1;
DE AltName: Full=Helix-loop-helix protein mATH-4C;
DE Short=mATH4C;
DE AltName: Full=Neurogenic basic-helix-loop-helix protein;
DE AltName: Full=Neurogenic differentiation factor 3;
DE Short=NeuroD3;
GN Name=Neurog1; Synonyms=Ath4c, Neurod3, Ngn, Ngn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=8816493; DOI=10.1128/mcb.16.10.5792;
RA McCormick M.B., Tamimi R.M., Snider L., Asakura A., Bergstrom D.,
RA Tapscott S.J.;
RT "NeuroD2 and neuroD3: distinct expression patterns and transcriptional
RT activation potentials within the neuroD gene family.";
RL Mol. Cell. Biol. 16:5792-5800(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=8858147; DOI=10.1016/s0092-8674(00)81321-5;
RA Ma Q., Kintner C., Anderson D.J.;
RT "Identification of neurogenin, a vertebrate neuronal determination gene.";
RL Cell 87:43-52(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=9108377; DOI=10.1242/dev.124.8.1611;
RA Cau E., Gradwohl G., Fode C., Guillemot F.;
RT "Mash1 activates a cascade of bHLH regulators in olfactory neuron
RT progenitors.";
RL Development 124:1611-1621(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=10648228; DOI=10.1242/dev.127.4.693;
RA Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.;
RT "Generation of neurons by transient expression of neural bHLH proteins in
RT mammalian cells.";
RL Development 127:693-702(2000).
RN [6]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=14697366; DOI=10.1016/j.ydbio.2003.08.027;
RA Lin C.H., Stoeck J., Ravanpay A.C., Guillemot F., Tapscott S.J.,
RA Olson J.M.;
RT "Regulation of neuroD2 expression in mouse brain.";
RL Dev. Biol. 265:234-245(2004).
RN [7]
RP FUNCTION, AND ASSOCIATION WITH CHROMATIN.
RX PubMed=18007592; DOI=10.1038/sj.emboj.7601923;
RA Seo S., Lim J.W., Yellajoshyula D., Chang L.W., Kroll K.L.;
RT "Neurogenin and NeuroD direct transcriptional targets and their regulatory
RT enhancers.";
RL EMBO J. 26:5093-5108(2007).
CC -!- FUNCTION: Acts as a transcriptional regulator. Involved in the
CC initiation of neuronal differentiation. Activates transcription by
CC binding to the E box (5'-CANNTG-3'). Associates with chromatin to
CC enhancer regulatory elements in genes encoding key transcriptional
CC regulators of neurogenesis. {ECO:0000269|PubMed:10648228,
CC ECO:0000269|PubMed:14697366, ECO:0000269|PubMed:18007592}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expression restricted to the embryonic nervous
CC system.
CC -!- DEVELOPMENTAL STAGE: Highest expression in the embryo between days 10
CC and 12. Declines to undetectable levels by embryonic day 16.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U63841; AAB37576.1; -; Genomic_DNA.
DR EMBL; U67776; AAC52856.1; -; Genomic_DNA.
DR EMBL; Y09166; CAA70365.1; -; Genomic_DNA.
DR EMBL; BC062148; AAH62148.1; -; mRNA.
DR CCDS; CCDS26559.1; -.
DR RefSeq; NP_035026.1; NM_010896.2.
DR AlphaFoldDB; P70660; -.
DR SMR; P70660; -.
DR BioGRID; 201735; 4.
DR IntAct; P70660; 1.
DR STRING; 10090.ENSMUSP00000050484; -.
DR iPTMnet; P70660; -.
DR PhosphoSitePlus; P70660; -.
DR PaxDb; P70660; -.
DR PRIDE; P70660; -.
DR Antibodypedia; 26408; 607 antibodies from 34 providers.
DR DNASU; 18014; -.
DR Ensembl; ENSMUST00000058475; ENSMUSP00000050484; ENSMUSG00000048904.
DR GeneID; 18014; -.
DR KEGG; mmu:18014; -.
DR UCSC; uc007qsl.2; mouse.
DR CTD; 4762; -.
DR MGI; MGI:107754; Neurog1.
DR VEuPathDB; HostDB:ENSMUSG00000048904; -.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000162170; -.
DR HOGENOM; CLU_097959_0_0_1; -.
DR InParanoid; P70660; -.
DR OMA; FAYGPGD; -.
DR OrthoDB; 1524429at2759; -.
DR PhylomeDB; P70660; -.
DR TreeFam; TF315153; -.
DR BioGRID-ORCS; 18014; 2 hits in 73 CRISPR screens.
DR PRO; PR:P70660; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P70660; protein.
DR Bgee; ENSMUSG00000048904; Expressed in cerebral cortex ventricular layer and 91 other tissues.
DR Genevisible; P70660; MM.
DR GO; GO:0043025; C:neuronal cell body; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043204; C:perikaryon; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070888; F:E-box binding; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0031223; P:auditory behavior; ISO:MGI.
DR GO; GO:0045165; P:cell fate commitment; IGI:MGI.
DR GO; GO:0090102; P:cochlea development; ISO:MGI.
DR GO; GO:0090103; P:cochlea morphogenesis; ISO:MGI.
DR GO; GO:0097094; P:craniofacial suture morphogenesis; ISO:MGI.
DR GO; GO:0010458; P:exit from mitosis; IMP:MGI.
DR GO; GO:0048806; P:genitalia development; ISO:MGI.
DR GO; GO:0035112; P:genitalia morphogenesis; ISO:MGI.
DR GO; GO:1905748; P:hard palate morphogenesis; ISO:MGI.
DR GO; GO:0048839; P:inner ear development; ISO:MGI.
DR GO; GO:0042472; P:inner ear morphogenesis; IMP:MGI.
DR GO; GO:0098583; P:learned vocalization behavior; ISO:MGI.
DR GO; GO:0071626; P:mastication; ISO:MGI.
DR GO; GO:1901078; P:negative regulation of relaxation of muscle; ISO:MGI.
DR GO; GO:1905747; P:negative regulation of saliva secretion; ISO:MGI.
DR GO; GO:0022008; P:neurogenesis; IDA:MGI.
DR GO; GO:0050885; P:neuromuscular process controlling balance; ISO:MGI.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0030432; P:peristalsis; ISO:MGI.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:MGI.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0048634; P:regulation of muscle organ development; ISO:MGI.
DR GO; GO:0045664; P:regulation of neuron differentiation; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007356; P:thorax and anterior abdomen determination; ISO:MGI.
DR GO; GO:0021559; P:trigeminal nerve development; ISO:MGI.
DR GO; GO:0021650; P:vestibulocochlear nerve formation; ISO:MGI.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032653; Ngn1.
DR PANTHER; PTHR19290:SF130; PTHR19290:SF130; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..244
FT /note="Neurogenin-1"
FT /id="PRO_0000127397"
FT DOMAIN 93..145
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 39..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 244 AA; 26300 MW; 3C9C0E1A08AB074B CRC64;
MPAPLETCIS DLDCSSSNSS SDLSSFLTDE EDCARLQPLA STSGLSVPAR RSAPALSGAS
NVPGAQDEEQ ERRRRRGRAR VRSEALLHSL RRSRRVKAND RERNRMHNLN AALDALRSVL
PSFPDDTKLT KIETLRFAYN YIWALAETLR LADQGLPGGS ARERLLPPQC VPCLPGPPSP
ASDTESWGSG AAASPCATVA SPLSDPSSPS ASEDFTYGPG DPLFSFPGLP KDLLHTTPCF
IPYH
