NGN2_MOUSE
ID NGN2_MOUSE Reviewed; 263 AA.
AC P70447; P70237;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Neurogenin-2;
DE Short=NGN-2;
DE AltName: Full=Helix-loop-helix protein mATH-4A;
DE Short=mATH4A;
DE AltName: Full=Protein atonal homolog 4;
GN Name=Neurog2; Synonyms=Ath4a, Atoh4, Ngn2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9000438; DOI=10.1006/mcne.1996.0060;
RA Sommer L., Ma Q., Anderson D.J.;
RT "Neurogenins, a novel family of atonal-related bHLH transcription factors,
RT are putative mammalian neuronal determination genes that reveal progenitor
RT cell heterogeneity in the developing CNS and PNS.";
RL Mol. Cell. Neurosci. 8:221-241(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD-1;
RX PubMed=8948587; DOI=10.1006/dbio.1996.0297;
RA Gradwohl G., Fode C., Guillemot F.;
RT "Restricted expression of a novel murine atonal-related bHLH protein in
RT undifferentiated neural precursors.";
RL Dev. Biol. 180:227-241(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=129;
RA Simmons A.D., Horton S., Abney A.L., Johnson J.E.;
RT "Neurogenin 2 expression in ventral and dorsal spinal neural tube
RT progenitor cells is regulated by distinct enhancers.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=14697366; DOI=10.1016/j.ydbio.2003.08.027;
RA Lin C.H., Stoeck J., Ravanpay A.C., Guillemot F., Tapscott S.J.,
RA Olson J.M.;
RT "Regulation of neuroD2 expression in mouse brain.";
RL Dev. Biol. 265:234-245(2004).
CC -!- FUNCTION: Transcriptional regulator. Involved in neuronal
CC differentiation. Activates transcription by binding to the E box (5'-
CC CANNTG-3'). {ECO:0000269|PubMed:14697366}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; U76207; AAC53028.1; -; Genomic_DNA.
DR EMBL; Y07621; CAA68900.1; -; mRNA.
DR EMBL; AF303001; AAG40769.1; -; Genomic_DNA.
DR CCDS; CCDS17825.1; -.
DR RefSeq; NP_033848.1; NM_009718.2.
DR AlphaFoldDB; P70447; -.
DR SMR; P70447; -.
DR BioGRID; 198240; 5.
DR STRING; 10090.ENSMUSP00000029587; -.
DR iPTMnet; P70447; -.
DR PhosphoSitePlus; P70447; -.
DR PaxDb; P70447; -.
DR PRIDE; P70447; -.
DR DNASU; 11924; -.
DR GeneID; 11924; -.
DR KEGG; mmu:11924; -.
DR CTD; 63973; -.
DR MGI; MGI:109619; Neurog2.
DR eggNOG; KOG3898; Eukaryota.
DR InParanoid; P70447; -.
DR OrthoDB; 1524429at2759; -.
DR BioGRID-ORCS; 11924; 3 hits in 76 CRISPR screens.
DR PRO; PR:P70447; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P70447; protein.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070888; F:E-box binding; IDA:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007411; P:axon guidance; IMP:MGI.
DR GO; GO:0021870; P:Cajal-Retzius cell differentiation; IDA:MGI.
DR GO; GO:0045165; P:cell fate commitment; IGI:MGI.
DR GO; GO:0048469; P:cell maturation; IMP:MGI.
DR GO; GO:0021954; P:central nervous system neuron development; IMP:MGI.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:MGI.
DR GO; GO:0030900; P:forebrain development; IMP:MGI.
DR GO; GO:0022008; P:neurogenesis; IDA:MGI.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0001764; P:neuron migration; IMP:MGI.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR DisProt; DP02061; -.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032655; Ngn2.
DR PANTHER; PTHR19290:SF105; PTHR19290:SF105; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..263
FT /note="Neurogenin-2"
FT /id="PRO_0000127401"
FT DOMAIN 112..164
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 20..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 60
FT /note="E -> G (in Ref. 2; CAA68900)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 28216 MW; 817EF8246BD8CABE CRC64;
MFVKSETLEL KEEEEVLMLL GSASPASATL TPMSSSADEE EDEELRRPGS ARGQRGAEAE
QGVQGSPASG AGGCRPGRLL GLMHECKRRP SRSRAVSRGA KTAETVQRIK KTRRLKANNR
ERNRMHNLNA ALDALREVLP TFPEDAKLTK IETLRFAHNY IWALTETLRL ADHCAGAGGL
QGALFTEAVL LSPGAALGAS GDSPSPPSSW SCTNSPASSS NSTSPYSCTL SPASPGSDVD
YWQPPPPEKH RYAPHLPLAR DCI
