NGN3_MOUSE
ID NGN3_MOUSE Reviewed; 214 AA.
AC P70661;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Neurogenin-3;
DE Short=NGN-3;
DE AltName: Full=Helix-loop-helix protein mATH-4B;
DE Short=mATH4B;
DE AltName: Full=Protein atonal homolog 5;
GN Name=Neurog3; Synonyms=Ath4b, Atoh5, Ngn3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=9108377; DOI=10.1242/dev.124.8.1611;
RA Cau E., Gradwohl G., Fode C., Guillemot F.;
RT "Mash1 activates a cascade of bHLH regulators in olfactory neuron
RT progenitors.";
RL Development 124:1611-1621(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9000438; DOI=10.1006/mcne.1996.0060;
RA Sommer L., Ma Q., Anderson D.J.;
RT "Neurogenins, a novel family of atonal-related bHLH transcription factors,
RT are putative mammalian neuronal determination genes that reveal progenitor
RT cell heterogeneity in the developing CNS and PNS.";
RL Mol. Cell. Neurosci. 8:221-241(1996).
RN [3]
RP FUNCTION.
RX PubMed=10677506; DOI=10.1073/pnas.97.4.1607;
RA Gradwohl G., Dierich A., LeMeur M., Guillemot F.;
RT "Neurogenin3 is required for the development of the four endocrine cell
RT lineages of the pancreas.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:1607-1611(2000).
RN [4]
RP INTERACTION WITH ATOH8.
RX PubMed=18560595; DOI=10.1371/journal.pone.0002430;
RA Lynn F.C., Sanchez L., Gomis R., German M.S., Gasa R.;
RT "Identification of the bHLH factor Math6 as a novel component of the
RT embryonic pancreas transcriptional network.";
RL PLoS ONE 3:E2430-E2430(2008).
RN [5]
RP FUNCTION.
RX PubMed=19759004; DOI=10.1074/jbc.m109.048694;
RA Anderson K.R., Torres C.A., Solomon K., Becker T.C., Newgard C.B.,
RA Wright C.V., Hagman J., Sussel L.;
RT "Cooperative transcriptional regulation of the essential pancreatic islet
RT gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3.";
RL J. Biol. Chem. 284:31236-31248(2009).
CC -!- FUNCTION: Acts as a transcriptional regulator. Together with NKX2-2,
CC initiates transcriptional activation of NEUROD1. Involved in
CC neurogenesis. Also required for the specification of a common precursor
CC of the 4 pancreatic endocrine cell types. {ECO:0000269|PubMed:10677506,
CC ECO:0000269|PubMed:19759004}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Interacts with ATOH8. {ECO:0000269|PubMed:18560595}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; Y09167; CAA70366.1; -; Genomic_DNA.
DR EMBL; U76208; AAC53029.1; -; Genomic_DNA.
DR CCDS; CCDS23887.1; -.
DR RefSeq; NP_033849.3; NM_009719.6.
DR AlphaFoldDB; P70661; -.
DR SMR; P70661; -.
DR STRING; 10090.ENSMUSP00000054054; -.
DR iPTMnet; P70661; -.
DR PhosphoSitePlus; P70661; -.
DR PaxDb; P70661; -.
DR PRIDE; P70661; -.
DR Antibodypedia; 28907; 558 antibodies from 33 providers.
DR DNASU; 11925; -.
DR Ensembl; ENSMUST00000050103; ENSMUSP00000054054; ENSMUSG00000044312.
DR GeneID; 11925; -.
DR KEGG; mmu:11925; -.
DR UCSC; uc007fgu.1; mouse.
DR CTD; 50674; -.
DR MGI; MGI:893591; Neurog3.
DR VEuPathDB; HostDB:ENSMUSG00000044312; -.
DR eggNOG; KOG3898; Eukaryota.
DR GeneTree; ENSGT00940000163077; -.
DR HOGENOM; CLU_097959_1_0_1; -.
DR InParanoid; P70661; -.
DR OMA; ADHSFYG; -.
DR OrthoDB; 1524429at2759; -.
DR PhylomeDB; P70661; -.
DR TreeFam; TF315153; -.
DR BioGRID-ORCS; 11925; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Neurog3; mouse.
DR PRO; PR:P70661; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; P70661; protein.
DR Bgee; ENSMUSG00000044312; Expressed in foregut-midgut junction and 45 other tissues.
DR ExpressionAtlas; P70661; baseline and differential.
DR Genevisible; P70661; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0031490; F:chromatin DNA binding; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003690; F:double-stranded DNA binding; ISO:MGI.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0031018; P:endocrine pancreas development; IEA:InterPro.
DR GO; GO:0030855; P:epithelial cell differentiation; IMP:MGI.
DR GO; GO:0030900; P:forebrain development; IEA:Ensembl.
DR GO; GO:0030902; P:hindbrain development; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:GO_Central.
DR GO; GO:0048814; P:regulation of dendrite morphogenesis; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0021510; P:spinal cord development; IEA:Ensembl.
DR GO; GO:0060290; P:transdifferentiation; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR032656; Ngn3.
DR PANTHER; PTHR19290:SF94; PTHR19290:SF94; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..214
FT /note="Neurogenin-3"
FT /id="PRO_0000127403"
FT DOMAIN 83..135
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 214 AA; 23267 MW; 1AB31183291FB98B CRC64;
MAPHPLDALT IQVSPETQQP FPGASDHEVL SSNSTPPSPT LIPRDCSEAE VGDCRGTSRK
LRARRGGRNR PKSELALSKQ RRSRRKKAND RERNRMHNLN SALDALRGVL PTFPDDAKLT
KIETLRFAHN YIWALTQTLR IADHSFYGPE PPVPCGELGS PGGGSNGDWG SIYSPVSQAG
NLSPTASLEE FPGLQVPSSP SYLLPGALVF SDFL
