NHAA1_PSESM
ID NHAA1_PSESM Reviewed; 392 AA.
AC Q87WL3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Na(+)/H(+) antiporter NhaA 1 {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA 1 {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA1 {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=PSPTO_4533;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01844}.
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DR EMBL; AE016853; AAO57981.1; -; Genomic_DNA.
DR RefSeq; NP_794286.1; NC_004578.1.
DR RefSeq; WP_011105042.1; NC_004578.1.
DR AlphaFoldDB; Q87WL3; -.
DR SMR; Q87WL3; -.
DR STRING; 223283.PSPTO_4533; -.
DR EnsemblBacteria; AAO57981; AAO57981; PSPTO_4533.
DR GeneID; 1186216; -.
DR KEGG; pst:PSPTO_4533; -.
DR PATRIC; fig|223283.9.peg.4651; -.
DR eggNOG; COG3004; Bacteria.
DR HOGENOM; CLU_015803_1_0_6; -.
DR OMA; WFFMLQS; -.
DR OrthoDB; 1805428at2; -.
DR PhylomeDB; Q87WL3; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR PANTHER; PTHR30341; PTHR30341; 1.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..392
FT /note="Na(+)/H(+) antiporter NhaA 1"
FT /id="PRO_0000334379"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ SEQUENCE 392 AA; 40730 MW; 84CC94BE5F12D0FE CRC64;
MHNPTPRTES PNALAFIKRF FAAEAAGGLI LMAAALAALI VANSPLADSY FAALHTVLAG
MSVEHWINDG LMAIFFMLVG LEIKREMLAG QLASWSQRAL PGFAALGGMV VPALIYVAFN
WGQPETIGGW AIPAATDIAF ALGVLSLLGK RVPLSLKIFL SALAILDDLG AVLIIALFYT
SDLSIPMLLA ALGSIAVLVA LNRLGVKKLL PYLLIGALLW FFMLQSGIHA TLAGVALALC
IPLGKADEEA SSPLLHLEEK LHPWVAFAVV PIFGFANAGV SLAGITVDKL VDPVPLGVAL
GLLIGKQVGI FALAALAIRA GLARLPDGSN WGQLYGVAAL CGIGFTMSLF IGALAFPGAP
ALVDEVKVGV LIGSVFSALL GVVVLRRFGA RG
