ID   NHAA_ALIF1              Reviewed;         382 AA.
AC   Q5E6G4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE   AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN   Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=VF_0887;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC         Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01844}.
CC   -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000020; AAW85382.1; -; Genomic_DNA.
DR   RefSeq; WP_011261552.1; NC_006840.2.
DR   RefSeq; YP_204270.1; NC_006840.2.
DR   AlphaFoldDB; Q5E6G4; -.
DR   SMR; Q5E6G4; -.
DR   STRING; 312309.VF_0887; -.
DR   EnsemblBacteria; AAW85382; AAW85382; VF_0887.
DR   KEGG; vfi:VF_0887; -.
DR   PATRIC; fig|312309.11.peg.883; -.
DR   eggNOG; COG3004; Bacteria.
DR   HOGENOM; CLU_015803_1_0_6; -.
DR   OMA; MGLMLRC; -.
DR   OrthoDB; 1805428at2; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR   Gene3D; 1.20.1530.10; -; 1.
DR   HAMAP; MF_01844; NhaA; 1.
DR   InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR   InterPro; IPR004670; NhaA.
DR   PANTHER; PTHR30341; PTHR30341; 1.
DR   Pfam; PF06965; Na_H_antiport_1; 1.
DR   TIGRFAMs; TIGR00773; NhaA; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..382
FT                   /note="Na(+)/H(+) antiporter NhaA"
FT                   /id="PRO_0000334458"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        321..341
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ   SEQUENCE   382 AA;  40250 MW;  68CE4797DDD1209A CRC64;
     MSDVIKNFFK LESAGGILLV IAAAIAMMIA NSSLAPMYDT FLHSYIGGMS VSHWINDGLM
     AVFFLLIGLE VKRELLEGAL KSKETAIFPA IAAVGGMLAP ALVYVAFNMG DPEALSGWAI
     PAATDIAFAL GIMALLGNRV PVSLKVFLLA LAIIDDLGVV VIIAFFYTSD LSVLALVIGF
     VMTGLLFLLN AKHVTKIRWY LLVGFILWVS VLQSGVHATL AGVVLGFAIP LKGNKGERSP
     LKHMEHALHP YVAFAILPVF AFANAGISLE GVSLDSLTTT LPLGVALGLF LGKPLGIFSF
     SYLAVKSGVA KLPTGVNMKH IFAVSVLCGI GFTMSIFISS LAFGGVNPEF DKLARLGILM
     GSTFAAVVGY ALLSISLPKK AA