ID   NHAA_BIFAA              Reviewed;         454 AA.
AC   A1A0Y8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE   AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN   Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=BAD_0590;
OS   Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 /
OS   E194a).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=367928;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15703 / DSM 20083 / NCTC 11814 / E194a;
RA   Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S.,
RA   Tanaka K., Watanabe K.;
RT   "Bifidobacterium adolescentis complete genome sequence.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC         Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01844};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01844}.
CC   -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01844}.
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DR   EMBL; AP009256; BAF39371.1; -; Genomic_DNA.
DR   AlphaFoldDB; A1A0Y8; -.
DR   SMR; A1A0Y8; -.
DR   STRING; 367928.BAD_0590; -.
DR   EnsemblBacteria; BAF39371; BAF39371; BAD_0590.
DR   KEGG; bad:BAD_0590; -.
DR   HOGENOM; CLU_015803_0_0_11; -.
DR   OMA; LHFITND; -.
DR   Proteomes; UP000008702; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR   Gene3D; 1.20.1530.10; -; 1.
DR   HAMAP; MF_01844; NhaA; 1.
DR   InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR   InterPro; IPR004670; NhaA.
DR   PANTHER; PTHR30341; PTHR30341; 1.
DR   Pfam; PF06965; Na_H_antiport_1; 1.
DR   TIGRFAMs; TIGR00773; NhaA; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell membrane; Ion transport; Membrane; Reference proteome;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..454
FT                   /note="Na(+)/H(+) antiporter NhaA"
FT                   /id="PRO_0000334239"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        355..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        386..406
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ   SEQUENCE   454 AA;  48414 MW;  65302DF8FDF4027E CRC64;
     MMATSKRGTW ATIRRIAASD RISGLIMLGF ALAGLLLANL PFTAHAFEQL ENFHIAIPHT
     NIDMGLGHWV QDGLLTVFFL TVGLELKQEL TTGSLSNPKA AAVPMLCAVG GMLAPPVLFI
     GVISLFAAAG SGQLVIASGT SFSFAQMSNG WAVPTATDIA FSLAVLALFA KALPGSIRAF
     LMTLATVDDL LAIILIAVFF SSVNAWYWFL GIAVCAVVWY FLVRMRKVPW LAVAIVGVLA
     WVMMFEAGVH PTLAGVLVGL LTPAHERFGE KTPRAERYAD KLQPFSALLA LPIFALFATG
     VHFESLTLAL FVSPVVVAVM VALVVGKPLG IMTTAWLSTH VGGLKMAKGL RVRDMFPAAC
     ACGIGFTVSF LIASLAYQDA ELSAEARFGV LVGSMVAAVI SGILLSRQSK RFELEDGTKH
     KHHTGDEDAM NEVETVLEDG TVVVSQIIGT HQEQ