NHAA_BIFBU
ID NHAA_BIFBU Reviewed; 462 AA.
AC Q2VPW7; F9XYN2;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=Bbr_0798;
OS Bifidobacterium breve (strain NCIMB 8807 / UCC2003).
OC Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC Bifidobacterium.
OX NCBI_TaxID=326426;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NCIMB 8807 / UCC2003;
RX PubMed=16321946; DOI=10.1128/jb.187.24.8411-8426.2005;
RA Ventura M., Zhang Z., Cronin M., Canchaya C., Kenny J.G., Fitzgerald G.F.,
RA van Sinderen D.;
RT "The ClgR protein regulates transcription of the clpP operon in
RT Bifidobacterium breve UCC 2003.";
RL J. Bacteriol. 187:8411-8426(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCIMB 8807 / UCC2003;
RX PubMed=21690406; DOI=10.1073/pnas.1105380108;
RA O'Connell Motherway M., Zomer A., Leahy S.C., Reunanen J., Bottacini F.,
RA Claesson M.J., O'Brien F., Flynn K., Casey P.G., Moreno Munoz J.A.,
RA Kearney B., Houston A.M., O'Mahony C., Higgins D.G., Shanahan F., Palva A.,
RA de Vos W.M., Fitzgerald G.F., Ventura M., O'Toole P.W., van Sinderen D.;
RT "Functional genome analysis of Bifidobacterium breve UCC2003 reveals type
RT IVb tight adherence (Tad) pili as an essential and conserved host-
RT colonization factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:11217-11222(2011).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01844};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01844}.
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DR EMBL; AY955251; AAY29675.1; -; Genomic_DNA.
DR EMBL; CP000303; ABE95483.1; -; Genomic_DNA.
DR RefSeq; WP_015438691.1; NC_020517.1.
DR AlphaFoldDB; Q2VPW7; -.
DR SMR; Q2VPW7; -.
DR STRING; 326426.Bbr_0798; -.
DR EnsemblBacteria; ABE95483; ABE95483; Bbr_0798.
DR KEGG; bbru:Bbr_0798; -.
DR PATRIC; fig|326426.4.peg.865; -.
DR eggNOG; COG3004; Bacteria.
DR HOGENOM; CLU_015803_0_0_11; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR PANTHER; PTHR30341; PTHR30341; 1.
DR Pfam; PF06965; Na_H_antiport_1; 1.
PE 3: Inferred from homology;
KW Antiport; Cell membrane; Ion transport; Membrane; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..462
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334240"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 256..275
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ SEQUENCE 462 AA; 48773 MW; 490852D8B17FFAF0 CRC64;
MATTTGAKRG IWPMIRRIAA SDRISGLIML GFALAGLVLA NLPLTAHAFE AVAETHVFIP
HTNLDLPIGH WAQDGLLTIF FLTVGLELKQ ELTTGSLANP KAAAVPMLCA VGGMITPPIL
FLATTALFSQ FGPGEPGSLI LATTGSSIPF AEMSHGWAVP TATDIAFSLA VLALFAKALP
GSIRAFLMTL ATVDDLLAII LIAVFFSSVN AWYWFIGIAV CAVVWAHLVR LKKVPWIAVG
VVGILAWIMM FEAGIHPTLA GVLVGLLTPA RVMHGEYSPR AERYADKLKP FSALLALPIF
ALFATGVHFE SMSPLLLLSP LVIALIVALV VGKPLGIIVT AWLATHVGGL KMAKGLRVRD
MIPAAVACGI GFTVSFLIAS LAYTNQELSA EARFGVLVAS LIAAAISGVL LSRQSKRFEQ
AAAVAAAEAD AESDVNTHSD ELAEHSITLA DGTESVEIDF RR
