NHAA_BRUA2
ID NHAA_BRUA2 Reviewed; 736 AA.
AC Q2YMB3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=BAB1_0425;
OS Brucella abortus (strain 2308).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the NhaA Na(+)/H(+)
CC (TC 2.A.33) antiporter family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the peptidase S49
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAJ10381.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AM040264; CAJ10381.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_002971575.1; NZ_KN046823.1.
DR AlphaFoldDB; Q2YMB3; -.
DR SMR; Q2YMB3; -.
DR STRING; 359391.BAB1_0425; -.
DR EnsemblBacteria; CAJ10381; CAJ10381; BAB1_0425.
DR GeneID; 3787184; -.
DR KEGG; bmf:BAB1_0425; -.
DR HOGENOM; CLU_020970_0_0_5; -.
DR PhylomeDB; Q2YMB3; -.
DR Proteomes; UP000002719; Chromosome I.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008233; F:peptidase activity; IEA:InterPro.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR CDD; cd07023; S49_Sppa_N_C; 1.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR InterPro; IPR002142; Peptidase_S49.
DR PANTHER; PTHR30341; PTHR30341; 2.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR Pfam; PF01343; Peptidase_S49; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..736
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334475"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT REGION 1..387
FT /note="Na(+)/H(+) antiporter NhaA"
FT REGION 388..736
FT /note="Peptidase S49"
SQ SEQUENCE 736 AA; 78587 MW; 7C5E5742B5EC5654 CRC64;
MNHSPQSARP VSIMRRFLDS EAAGGITLMA AAALALIVAN SPFAQTYFDA LHLYIGPLSL
AHWINDALMA IFFLLVGLEI KREMLDGQLA SWPNRMLPGI AAAGGVILPA IIFAVLNHDN
PAKLRGWAVP SATDIAFALG VLSLLGSRAP SSLKVFLATL AILDDLAAVV IIAIFYTAEI
SMPYLGAAFI TAAVLFVMNR MGVVKLLPYL ISAVILWFFV FNSGVHATVA GVVAALMIPL
KPAPGRPDDM TSPLHKLEHA LAKPVAFIVV PIFGFANAGI SFKGLEASVL GDTLTLGILL
GLFLGKQFGV FGAAWLAIKT GLAEKPMGAS WVQLYGVAIL CGIGFTMSIF IGLLSFPSDL
MQTETKIGVL SGSALSAICG YLLLRAARPD QSAANPLWKA DESPEAKNFG RFLCVFHFAS
YIASTYCTER LQAASSLLRR SSLEFALPGL LKRLIPRRFR AVETEIPVVR LHGAIMTGGT
SLRPTLSLAS TAGILEKAFA DKHAPAVAIS INSPGGAPVQ SRLIYRRIRD LAVEHQKKVF
VFVEDVAASG GYMIALAGDE IIADPSSIVG SIGVVSASFG FPELLKKIGV ERRVYTAGSN
KVTLDPFQPE KAEDIERLKA LQLEIHATFI DMVKERRAGK LGDNPDLFSG LFWTGTTAAS
LGLIDGLGDM RSFLRKTYGD KVKLKLIQPQ RGLLGRKLPG IGMDSGSVEP AQIAAHLGDG
LLCVAEEKAI WARYGL
