NHAA_BRUME
ID NHAA_BRUME Reviewed; 736 AA.
AC Q8YFI5;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=BMEI1535;
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA Haselkorn R., Kyrpides N.C., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the NhaA Na(+)/H(+)
CC (TC 2.A.33) antiporter family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the peptidase S49
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL52716.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE008917; AAL52716.1; ALT_INIT; Genomic_DNA.
DR PIR; AI3443; AI3443.
DR RefSeq; WP_005969858.1; NZ_GG703778.1.
DR AlphaFoldDB; Q8YFI5; -.
DR SMR; Q8YFI5; -.
DR STRING; 224914.BMEI1535; -.
DR EnsemblBacteria; AAL52716; AAL52716; BMEI1535.
DR GeneID; 29594383; -.
DR KEGG; bme:BMEI1535; -.
DR eggNOG; COG0616; Bacteria.
DR eggNOG; COG3004; Bacteria.
DR PhylomeDB; Q8YFI5; -.
DR Proteomes; UP000000419; Chromosome I.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008233; F:peptidase activity; IEA:InterPro.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR CDD; cd07023; S49_Sppa_N_C; 1.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR InterPro; IPR002142; Peptidase_S49.
DR PANTHER; PTHR30341; PTHR30341; 2.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR Pfam; PF01343; Peptidase_S49; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..736
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334476"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT REGION 1..387
FT /note="Na(+)/H(+) antiporter NhaA"
FT REGION 388..736
FT /note="Peptidase S49"
SQ SEQUENCE 736 AA; 78574 MW; 34AABFDFD80E525C CRC64;
MNHSPQSARP VSIMRRFLDS EAAGGITLMA AAALALIVAN SPFAQTYFDA LHLYIGPLSL
AHWINDALMA IFFLLVGLEI KREMLDGQLA SWPNRMLPGI AAAGGVILPA IIFAVLNHDN
PAKLRGWAVP SATDIAFALG VLSLLGSRAP SSLKVFLATL AILDDLAAVV IIAIFYTAEI
SMPYLGAAFI TAAVLFVMNR MDVVKLLPYL ISAVILWFFV FNSGVHATVA GVVAALMIPL
KPAPGRPDDM TSPLHKLEHA LAKPVAFIVV PIFGFANAGI SFKGLEASVL GDTLTLGILL
GLFLGKQFGV FGAAWLAIKT GLAEKPMGAS WVQLYGVAIL CGIGFTMSIF IGLLSFPSDL
MQTETKIGVL SGSALSAICG YLLLRAARPD QSAANPLWKA DESPEAKNFG RFLCVFHFAS
YIASTYCTER LQAASSLLRR SSLEFALPGL LKRLIPRRFR AVETEIPVVR LHGAIMTGGT
SLRPTLSLAS TAGILEKAFA DKHAPAVAIS INSPGGAPVQ SRLIYRRIRD LAAEHQKKVF
VFVEDVAASG GYMIALAGDE IIADPSSIVG SIGVVSASFG FPELLKKIGV ERRVYTAGSN
KVTLDPFQPE KAEDIERLKA LQLEIHATFI DMVKERRAGK LGDNPDLFSG LFWTGTTAAS
LGLIDGLGDM LSFLRKTYGD KVKLKLIQPQ RGLLGRKLPG IGMDSGSVEP AQIAAHLGDG
LLCVAEEKAI WARYGL
