ID   NHAA_CUPNH              Reviewed;         468 AA.
AC   Q0K437;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE   AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN   Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=H16_B0439;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC         Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01844}.
CC   -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01844}.
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DR   EMBL; AM260480; CAJ95237.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q0K437; -.
DR   SMR; Q0K437; -.
DR   STRING; 381666.H16_B0439; -.
DR   EnsemblBacteria; CAJ95237; CAJ95237; H16_B0439.
DR   KEGG; reh:H16_B0439; -.
DR   eggNOG; COG3004; Bacteria.
DR   HOGENOM; CLU_015803_1_2_4; -.
DR   OMA; WHTPFGF; -.
DR   Proteomes; UP000008210; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR   Gene3D; 1.20.1530.10; -; 1.
DR   HAMAP; MF_01844; NhaA; 1.
DR   InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR   InterPro; IPR004670; NhaA.
DR   PANTHER; PTHR30341; PTHR30341; 1.
DR   Pfam; PF06965; Na_H_antiport_1; 1.
DR   TIGRFAMs; TIGR00773; NhaA; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..468
FT                   /note="Na(+)/H(+) antiporter NhaA"
FT                   /id="PRO_0000334386"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        320..340
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        397..417
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT   TRANSMEM        428..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ   SEQUENCE   468 AA;  49452 MW;  3E139645FB6EE0D9 CRC64;
     MDRLPPPPPP PRQPLPRAQV LAEKAFSALE HFLHIEAVSG IVLLAAAGIA LLWANSPASH
     SYHALWHTPL SFGIGSHLVS QSLHFWINDA LMTIFFLVVG MEIRREIHEG ALANVRLSAL
     PMAAALGGVI VPALIYLAIN TEMPQRQGWA VPTATDIAFA VGVLALLGKS IPGNVRVFLL
     ALAIIDDIVA VLIIAVAFSG GLDYGGFLVA GTGILMVLGL QWIGVGTAYA YVVPGAILWL
     GMLKTGAHPT LAGVVLGMMT PVLPGRTRER PLDVAMRALL DLRARATART PKPEHIAAPL
     KQLRHAQREL LPPVIRVQMA LHPWVAYLVM PLFALANAGV SIDGVDLTSG GSLGAMFGVV
     LALVVGKPLG IVSVSWLAVR LGWCQLPPDV TWRGVCLVGL LAGIGFTMSI FIATLAFEDV
     NLLGAAKLGV LLASAIAATI GLTWGFIYAR GRRPSMERAD ESTAAASD