NHAA_NITMU
ID NHAA_NITMU Reviewed; 624 AA.
AC Q2Y8T1;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=Nmul_A1537;
OS Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosospira.
OX NCBI_TaxID=323848;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25196 / NCIMB 11849 / C 71;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M.,
RA Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC
RT 25196.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01844, ECO:0000305}.
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DR EMBL; CP000103; ABB74840.1; -; Genomic_DNA.
DR RefSeq; WP_011380869.1; NZ_FNVK01000015.1.
DR AlphaFoldDB; Q2Y8T1; -.
DR SMR; Q2Y8T1; -.
DR STRING; 323848.Nmul_A1537; -.
DR EnsemblBacteria; ABB74840; ABB74840; Nmul_A1537.
DR KEGG; nmu:Nmul_A1537; -.
DR eggNOG; COG1651; Bacteria.
DR eggNOG; COG3004; Bacteria.
DR HOGENOM; CLU_015803_4_0_4; -.
DR OMA; TFFFFIV; -.
DR OrthoDB; 1805428at2; -.
DR Proteomes; UP000002718; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR InterPro; IPR012336; Thioredoxin-like_fold.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR30341; PTHR30341; 1.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR Pfam; PF13462; Thioredoxin_4; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..624
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334483"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 497..517
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 565..585
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 596..616
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT REGION 1..164
FT /note="Unknown"
FT REGION 165..624
FT /note="Na(+)/H(+) antiporter NhaA"
SQ SEQUENCE 624 AA; 66846 MW; C33D8D2AFDBBF992 CRC64;
MNPELPPNHL DKPVDDAYDH TLGPADAEIT LVEYGSYADA PSRSAHERVA ELRSRFGNRM
RYVFRHRPLA GSKIARRAAE LVESHNNSGR FWDLHVALMS RSDKLSADDL CTIISDLKLE
GNKEAGQEET AERARDRVEA DIASANASGV IVTPTFFING RRYDGPWDVR SLSEAMLGSL
GHVVHAAALD FAKWAPSTGI MLLLATALAV VLSNSALGPD FNEMWEQRLG ITFQHSAFEL
SLRHWINDGL LVIFFLVVGL EIKREFTVGH LAERQSAMLP IAAAIGGMAV PALLYLILVP
PGPPMAPGSD GTDGAWSYGW GVPMATDTAF AIALIAMMGK RVPVELRVFL TAAAIVDDIG
AIIVVAIFYS GELHIAYLGS AVAIAGLLAL LNKGGVYRAS PYVILGIVLW VFVYASGIHA
TLAGIILALF IPTRPPPNLR ALMLQADAIL TAETRRGREV MHYGPSEPAL EALDAIHDRL
ESPAARMLRH LAPRSSFLVL PVFALANAGV VVETSVFGEH IPLMLGTATA LVIGKPLGFI
TATVTAVRLG FATKPDAYSW RQLGGAGALA GIGFTMSLFI ASQAFPEVSD YAAAKIAIFG
GSILSAIIGV AILWNAQAEE EKNP
