NHAA_SHIDS
ID NHAA_SHIDS Reviewed; 388 AA.
AC Q32KA0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844}; OrderedLocusNames=SDY_0018;
OS Shigella dysenteriae serotype 1 (strain Sd197).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=300267;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sd197;
RX PubMed=16275786; DOI=10.1093/nar/gki954;
RA Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J.,
RA Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J.,
RA Xu J., Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J.,
RA Jin Q.;
RT "Genome dynamics and diversity of Shigella species, the etiologic agents of
RT bacillary dysentery.";
RL Nucleic Acids Res. 33:6445-6458(2005).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000034; ABB60258.1; -; Genomic_DNA.
DR RefSeq; WP_000681370.1; NC_007606.1.
DR RefSeq; YP_401746.1; NC_007606.1.
DR AlphaFoldDB; Q32KA0; -.
DR SMR; Q32KA0; -.
DR STRING; 300267.SDY_0018; -.
DR EnsemblBacteria; ABB60258; ABB60258; SDY_0018.
DR KEGG; sdy:SDY_0018; -.
DR PATRIC; fig|300267.13.peg.19; -.
DR HOGENOM; CLU_015803_1_0_6; -.
DR OMA; MGLMLRC; -.
DR Proteomes; UP000002716; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR PANTHER; PTHR30341; PTHR30341; 1.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..388
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334436"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 12..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 32..58
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 59..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 81..96
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 97..116
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 117..122
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 123..130
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 131..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 155..176
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 177..180
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 181..200
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 201..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 205..222
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 223
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 224..236
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 237..253
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 254..272
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 273..286
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 287..310
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 311..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 340..350
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 351..357
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 358..380
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 381..388
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ SEQUENCE 388 AA; 41378 MW; 127D8848B4E6EA91 CRC64;
MKHLHRFFSS DASGGIILII AAILAMMMAN SGATSGWYHD FLETPVQLRV GSLEINKNML
LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA AIGGMIVPAL LYLAFNYADP
ITREGWAIPA ATDIAFALGV LALLGSRVPL ALKIFLMALA IIDDLGAIII IALFYTNDLS
MASLGVAAVA IAVLAVLNLC GVRRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK
EKHGRSTAKR LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK
PLGISLFCWL ALRLKLAHLP EGTTYQQIMA VGILCGIGFT MSIFIASLAF GSVDPELINW
AKLGILVGSI SSAVIGYSWL RVRLRPSV
