NHAA_SHIFL
ID NHAA_SHIFL Reviewed; 388 AA.
AC Q83SR3; Q7C3C6;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN OrderedLocusNames=SF0016, S0018;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01844}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01844}.
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DR EMBL; AE005674; AAN41682.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP15563.1; -; Genomic_DNA.
DR RefSeq; NP_705975.1; NC_004337.2.
DR RefSeq; WP_000681363.1; NZ_UIQL01000020.1.
DR AlphaFoldDB; Q83SR3; -.
DR SMR; Q83SR3; -.
DR STRING; 198214.SF0016; -.
DR EnsemblBacteria; AAN41682; AAN41682; SF0016.
DR EnsemblBacteria; AAP15563; AAP15563; S0018.
DR GeneID; 1027500; -.
DR GeneID; 58388839; -.
DR KEGG; sfl:SF0016; -.
DR KEGG; sft:NCTC1_00018; -.
DR KEGG; sfx:S0018; -.
DR PATRIC; fig|198214.7.peg.16; -.
DR HOGENOM; CLU_015803_1_0_6; -.
DR OMA; MGLMLRC; -.
DR OrthoDB; 1805428at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR PANTHER; PTHR30341; PTHR30341; 1.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..388
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334437"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 12..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 32..58
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 59..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 81..96
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 97..116
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 117..122
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 123..130
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 131..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 155..176
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 177..180
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 181..200
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 201..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 205..222
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 223
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 224..236
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 237..253
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 254..272
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 273..286
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 287..310
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 311..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 340..350
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 351..357
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 358..380
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TOPO_DOM 381..388
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ SEQUENCE 388 AA; 41412 MW; 5C3D1F0E8053E361 CRC64;
MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV GSLEINKNML
LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA AIGGMIVPAL LYLAFNYADP
ITREGWAIPA ATDIAFALGV LALLGSRVPL VLKIFLMALA IIDDLGAIII IALFYTNDLS
MASLGVAAVA IAVLAVLNLC GVRRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK
EKHGRSPAKR LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK
PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF GSVDPELINW
AKLGILVGSI SSAVIGYSWL RVRLRPSV
