NHAA_VIBCH
ID NHAA_VIBCH Reviewed; 382 AA.
AC O85187; Q7DCU3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Na(+)/H(+) antiporter NhaA {ECO:0000303|PubMed:10781565};
DE AltName: Full=Sodium/proton antiporter NhaA {ECO:0000255|HAMAP-Rule:MF_01844};
GN Name=nhaA {ECO:0000303|PubMed:10781565}; OrderedLocusNames=VC_1627;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS ANTIPORTER, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=N18 / Serotype O1;
RX PubMed=10781565; DOI=10.1128/jb.182.10.2937-2944.2000;
RA Vimont S., Berche P.;
RT "NhaA, an Na(+)/H(+) antiporter involved in environmental survival of
RT Vibrio cholerae.";
RL J. Bacteriol. 182:2937-2944(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [3]
RP FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=N18 / Serotype O1;
RX PubMed=12562793; DOI=10.1128/jb.185.4.1236-1244.2003;
RA Herz K., Vimont S., Padan E., Berche P.;
RT "Roles of NhaA, NhaB, and NhaD Na(+)/H(+) antiporters in survival of Vibrio
RT cholerae in a saline environment.";
RL J. Bacteriol. 185:1236-1244(2003).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons (PubMed:10781565, PubMed:12562793). Can also transport
CC lithium (PubMed:12562793). Contributes to the survival of V.cholerae
CC during the stationary and exponential growth phases in a saline
CC environment (PubMed:12562793). {ECO:0000269|PubMed:10781565,
CC ECO:0000269|PubMed:12562793}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29251, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29252;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01844};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Li(+)(in) = 2 H(+)(in) + Li(+)(out);
CC Xref=Rhea:RHEA:70431, ChEBI:CHEBI:15378, ChEBI:CHEBI:49713;
CC Evidence={ECO:0000305|PubMed:12562793};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70432;
CC Evidence={ECO:0000305|PubMed:12562793};
CC -!- ACTIVITY REGULATION: Activity is regulated by pH (PubMed:12562793).
CC Active at alkaline pH (PubMed:12562793). {ECO:0000269|PubMed:12562793}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.65 mM for Na(+) {ECO:0000269|PubMed:12562793};
CC KM=0.052 mM for Li(+) {ECO:0000269|PubMed:12562793};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01844}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01844}.
CC -!- INDUCTION: Induced by sodium, lithium and potassium.
CC {ECO:0000269|PubMed:10781565}.
CC -!- DISRUPTION PHENOTYPE: The growth of the inactivated mutant is not
CC restricted at various pH and NaCl concentrations, although it is
CC inhibited in the presence of LiCl at pH 8.5.
CC {ECO:0000269|PubMed:10781565}.
CC -!- SIMILARITY: Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01844, ECO:0000305}.
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DR EMBL; AF051158; AAC33562.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF94778.1; -; Genomic_DNA.
DR PIR; B82177; B82177.
DR RefSeq; NP_231264.1; NC_002505.1.
DR RefSeq; WP_001281946.1; NZ_LT906614.1.
DR AlphaFoldDB; O85187; -.
DR SMR; O85187; -.
DR STRING; 243277.VC_1627; -.
DR DNASU; 2613882; -.
DR EnsemblBacteria; AAF94778; AAF94778; VC_1627.
DR GeneID; 57740283; -.
DR KEGG; vch:VC_1627; -.
DR PATRIC; fig|243277.26.peg.1555; -.
DR eggNOG; COG3004; Bacteria.
DR HOGENOM; CLU_015803_1_0_6; -.
DR OMA; MGLMLRC; -.
DR BioCyc; VCHO:VC1627-MON; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR Gene3D; 1.20.1530.10; -; 1.
DR HAMAP; MF_01844; NhaA; 1.
DR InterPro; IPR023171; Na/H_antiporter_dom_sf.
DR InterPro; IPR004670; NhaA.
DR PANTHER; PTHR30341; PTHR30341; 1.
DR Pfam; PF06965; Na_H_antiport_1; 1.
DR TIGRFAMs; TIGR00773; NhaA; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..382
FT /note="Na(+)/H(+) antiporter NhaA"
FT /id="PRO_0000334456"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01844"
SQ SEQUENCE 382 AA; 40316 MW; 547A5E0586C525E4 CRC64;
MSDMIRDFFK MESAGGILLV IAAAIAMVIA NSAMGEGYQA FLHTYVFGMS VSHWINDGLM
AVFFLLIGLE VKRELLEGAL KSRETAIFPA IAAVGGMLAP ALIYVAFNFN DPAAIQGWAI
PAATDIAFAL GIMALLGKRV PVSLKVFLLA LAIIDDLGVV VIIALFYSSD LSTIALTIGF
IMTGVLFMLN AKHVTKLSIY LVAGLILWIA VLKSGVHATL AGVVIGFAIP LKGNKGEHSP
LKHLEHALHP YVAFAILPVF AFANAGISLQ GVSLAGLTSM LPLGVALGLF LGKPLGIFSF
SWAAVKLGVA KLPEGINFKH IFAVSVLCGI GFTMSIFISS LAFGQANEAY DTYARLGILM
GSTTAALLGY SLLRLSLPLK KA
